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7 SCIENCE - Chap 5 - Lessons 1-3
7 SCIENCE - Chap 5 - Lessons 1-3

... Every time a cell divides, all chromosomes must be copied for the new cell. The new DNA is identical to existing DNA. Replication: the process of copying a DNA molecule to make another DAN molecule. The steps of DNA replication: 1. DNA strand separates and nitrogen bases are exposed. 2. Nucleotides ...
Evidence of Evolution - David Brotherton CCCMC
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... Gel Electrophoresis: Process that allows scientists to compare DNA from different organisms. DNA fingerprint: A pattern produced by gel electrophoresis.  The more similar the pattern, the more related the organisms. ...
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... sequence of nucleotides that forms part of a DNA molecule • Describe the way in which the nucleotide sequence codes for the amino acid sequence in the polypeptide • Describe the effects of substitution, deletion, insertion, and frameshift mutations • Describe how the information is used during trans ...
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Hoku`s Slides

... Several coupled DNA and protein libraries are constructed, randomizing 3 base pairs and 5 contacting amino acids for each NNNGGAGGTTTCTCTGTAAA TGANNNGGTTTCTCTGTAAA ...
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DNA (Deoxyribonucleic Acid)

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Name: ____________ Pd.: ______ Date: plasmid genetic

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this lecture as PDF here

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Genetics Learning Goals

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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