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talk_DNAEditing
talk_DNAEditing

... 4. Insertion into new genomic locations, increasing the number of genomic copies of the sequence. • Mobile elements are like double edge sword. ...
GENETICS 310
GENETICS 310

... IV.    Below  are  7  family  pedigrees  labeled  A  to  G  where  individuals  with  a  genetic  trait  are   filled  in  squares  or  circles.    List  the  pedigrees  could  potentially  result  from  a  trait  inherited   throu ...
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... Genotype – Rr, RR, rr (2 alleles per gene) Phenotype – tall or short – what you see! vocab - know it! allele, gene, hybrid, pure, trait, P1, F1, F2 4 step cross GASR working backwards - showing WORK! Dominance (RR, Rr – round, rr - wrinkled) Try to use letters that have different cases, Tt, Qq, etc. ...
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... All modern agricultural crops and livestock are the result of genetic modification, first through the  domestication process and in recent times through the application of scientific breeding (hybridization  and selection, induced mutations and selection).  Thus in plain English, all of agriculture  ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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