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CH 3 GENETICS - TEST – GIFT GUIDE HINTS due
CH 3 GENETICS - TEST – GIFT GUIDE HINTS due

... ** Be able to complete Punnett Squares and answer questions about them. ** Be able to match the main vocab terms with the definitions and/or concepts they represent Alleles = are different forms of a gene. (Examples: tall or short) Amino acids = link together into a chain to make proteins when tRNA ...
Human Genome Project and Cloning and
Human Genome Project and Cloning and

... codes for proteins, how many genes are there? Before the project began, scientists predicted that human cells would contain about 120,000 genes. In reality, researchers found only 30-40,000 genes in each cell. This is only about double the number of genes in a fruit fly. ...
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plasmid to transform

... a. The smaller fragments migrate further than the longer fragments. b. The bands are compared to standard DNA of known sizes. This is often called a DNA marker, or a DNA ...
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Lecture 6 Quiz

... 4. Which of the correct functions defined in the previous exercise is the fastest? Hint. You will need to generate a very large string to test them on, and the function clock() from the time module to time each function. ...
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Powerpoint file

... 3. A low concentration of DNase I is used so that on average each DNA molecule is cleaved just once (vertical arrows). 4. The two samples of DNA then are separated from protein, denatured to separate the strands, and electrophoresed. The resulting gel is analyzed by autoradiography, which detects on ...
Microbial Genetics
Microbial Genetics

... by a simple pathway: DNA— >RNA-->protein, which demonstrates the flow of genetic information in a living cell. ...
Chapter 20 Notes: DNA Technology
Chapter 20 Notes: DNA Technology

... Single-stranded DNA ends that are available to hydrogen bond to a complimentary single strand DNA bases that are added to a PCR machine so that multiple exact copies of a DNA sequence can be produced Segments of DNA that act as a probe in order to diagnose a genetic condition ...
Chapter 23 Lecture PowerPoint
Chapter 23 Lecture PowerPoint

... Phage coat is made of protein Always has the same volume DNA is much denser than protein More DNA in phage, denser phage Extra DNAs that can inactivate a gene by inserting into the gene were the first transposons discovered in bacteria • These transposons are called insertion sequences (ISs) ...
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Genetics practice test

... resistance of rare African rice varieties with the productivity and faster maturity of common Asian varieties. Another variety, called Stress-Tolerant Rice, was produced by inserting a pair of bacterial genes into rice plants for the production of trehalose (a sugar). Trehalose helps plants maintain ...
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Genetic Technology - Solon City Schools
Genetic Technology - Solon City Schools

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Gene expression and DNA microarrays

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... pUC and b-galactosidase a-complementation Plasmid contains part of the lacZ gene coding for the Nterminal extremity of the b-galactosidase enzyme. When expressed in E. coli lacZ strain = no activity Host bacterial strain contains a truncated lacZ gene encoding a polypeptide missing the N-terminal e ...
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Exp.9 Bacterial Transformation

... Plasmid DNA usually contains genes for one or more traits that may be beneficial to bacterial survival.  In nature, bacteria can transfer plasmids back and forth allowing them to share these beneficial genes.  This natural mechanism allows bacteria to adapt to new environments.  The occurrence of ...
Exp.9 Bacterial Transformation
Exp.9 Bacterial Transformation

... Plasmid DNA usually contains genes for one or more traits that may be beneficial to bacterial survival.  In nature, bacteria can transfer plasmids back and forth allowing them to share these beneficial genes.  This natural mechanism allows bacteria to adapt to new environments.  The occurrence of ...
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... 2. The deciduous forest regions are exposed to warm and cold air masses, which cause this area to have four seasons. The average yearly temperature is about 10°C. The areas in which deciduous forests are located get about 750 to 1,500 mm of precipitation spread fairly evenly throughout the year. Mos ...
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... • Replication – the synthesis of new DNA from existing DNA in the nucleus • DNA polymerase assembles new DNA strand and proofreads it • Replication occurs in nucleus prior to cell division ...
SoonChunHyang University: SoonChunHyang Institute of Medi
SoonChunHyang University: SoonChunHyang Institute of Medi

... Course Description : The course objective is to consider both principles and current topics in Molecular Biology in depth. This course primarily deals with nucleic acids and proteins and how these molecules interact within the cell to promote proper growth, division, and development. Especially this ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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