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Guided notes 2013 Sections 1 and 2 KEY
Guided notes 2013 Sections 1 and 2 KEY

... 1. DNA is digested with restriction enzymes. 2. This results in fragments of different lengths. Each person has a different pattern of fragment lengths. 3. Fragments are separated by gel electrophoresis. Shorter fragments travel farther. 4. The fragments are transferred to special paper and mixed wi ...
Station #3: DNA structure, replication, protein synthesis, mutation
Station #3: DNA structure, replication, protein synthesis, mutation

... Directions: Use the following information to write a conclusion Mike and Kelsey have a male and a female brown mouse with long tails. Question: What will the offspring of two mice who have brown fur and long tails look like? Prediction: If two brown mice mate, their offspring will all be brown with ...
Chapters 8-10
Chapters 8-10

... 8. Which of the following statements regarding genotypes and phenotypes is FALSE? A) The genetic makeup of an organism constitutes its genotype. B) An organism with two different alleles for a single trait is said to be heterozygous for that trait. C) Alleles are alternate forms of a gene. D) An all ...
AP Biology (An Introduction)
AP Biology (An Introduction)

... 3. Combine the 2 DNA pieces (into a recombinant plasmid?)  Recombinant plasmid – plasmid + DNA fragments  Sealed together using DNA Ligase  Remember: we used ________ ________ to cut gene of ...
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... RNA polymerase acts here ...
Chapter 8
Chapter 8

... characteristics of a cell; use this information synthesize proteins. 2. What four functions are performed by nucleic acids? 1) store information that determines the characteristics of cells and organisms; 2) direct the synthesis of proteins essential to the operation of the cell or organism; 3) chem ...
glossary of terms - Personal Genome Diagnostics
glossary of terms - Personal Genome Diagnostics

... A frameshift mutation is a type of mutation involving the insertion or deletion of a nucleotide in which the number of deleted base pairs is not divisible by three. “Divisible by three” is important because the cell reads a gene in groups of three bases. Each group of three bases corresponds to one ...
Chap 10 – DNA Structure
Chap 10 – DNA Structure

... Yet able to change (everyone has a unique genetic sequence) ...
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Molecular Genetics

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Transcription and Translation Eukaryotic Cell

... Amino Acid- Organic molecule possessing both carboxyl and amino groups. Serve as monomers of proteins. mRNA- is a single-stranded polymer of nucleotides, each of which contains a nitrogenous base, a sugar and a phosphate group. Messenger RNA contains genetic information. It carries genetic informati ...
DNA and RNA
DNA and RNA

... The blood of the dead mice showed high levels of virulent pneumococcus. Griffith theorized that some type of transformation takes place from the virulent to the non-virulent strain for it to synthesize a new polysaccharide coat. ...
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Spring 2011 Midterm Review Answers

... better suited to their environment survive and reproduce most successfully – they show high fitness.  Organisms have a struggle for existence – this must compete with other members of their species and with other species for food, living space, and other necessities of ...
Punnett Practice and Notes
Punnett Practice and Notes

...  These characteristics are called traits. Traits depend on the types of proteins that the 4 bases (A,C,G,T) make up. Parents pass on copies of their DNA to their offspring.  The DNA from each parent combines to form the DNA of the offspring.  How the offspring develops depends on the instructions ...
82. The Double Helix
82. The Double Helix

... Color the heading Structural Formula and the remainder of the plate. The structural formula shows more clearly which atoms are attached to which. These attachments are important to the cell because any deviation will result in some kind of mutation or even the death of the cell. To clarify the exact ...
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TARGETING YOUR DNA WITH THE CRE/LOX SYSTEM

... front of the gene that functions to recruit the cellular machinery that will initiate the multi-step process of protein production (called gene expression). How the promoter functions to do this can vary, from always recruiting cellular machinery and thus always being ‘on’, to only doing this in spe ...
Understanding DNA Technology
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... What is the role of DNA? Animals and plants are made up of cells. Most cells have a nucleus, which contains the chromosomes that hold the genetic blueprint for all living cells. Chromosomes are composed of deoxyribose nucleic acid, or DNA. One copy of each chromosome pair is inherited from each pare ...
DNA Replication
DNA Replication

... Just before the first nuclear division, homologous chromosomes pair along their lengths, so that now for each chromosomal type there are two pairs of sister chromatids juxtaposed, making a bundle of four also called a tetrad. At the tetrad stage a remarkable process occurs: paired nonsister chromati ...
DNA Workshop - Mrs. Sills` Science Site
DNA Workshop - Mrs. Sills` Science Site

... 23. What process occurs at the ribosomes? ___________________________________. 24. What is the name of the first amino acid created? ______________________________. Click “ok” and match the second anticodon with its codon. 25. What happens to the tRNA after it deposits its amino acid? ______________ ...
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... Copying DNA Genetic engineers can transfer a gene from one organism to another to achieve a goal, but first, individual genes must be identified and separated from DNA. The original method (used by Douglas Prasher) involved several steps: Determine the amino acid sequence in a protein. Predict the m ...
Chapter 9 I am - Mrs Smith`s Biology
Chapter 9 I am - Mrs Smith`s Biology

... I am the two parts of the DNA strand that connect to give the ‘backbone’ of the DNA strand before the daughter strands wind up into a double helix ...
Final Exam Study Guide Ms. Thomas Spring 2011
Final Exam Study Guide Ms. Thomas Spring 2011

... 16. Draw the following cycles and define each process within the cycle: a. Water b. Carbon c. Nitrogen 17. How many chromosomes are present in a human sex cell? 18. How much energy is transferred from one trophic level to the next? 19. List the differences between mitosis and meiosis. 20. List the t ...
15.2 Study Workbook
15.2 Study Workbook

... Copying DNA Genetic engineers can transfer a gene from one organism to another to achieve a goal, but first, individual genes must be identified and separated from DNA. The original method (used by Douglas Prasher) involved several steps: Determine the amino acid sequence in a protein. Predict the m ...
DNA - E. R. Greenman
DNA - E. R. Greenman

... • Worked with Colin MacLeod and Maclyn McCarty at Rockefeller University Hospital in 1943 • Treated S bacteria with protease, destroying protein, then added to R strain • Treated S bacteria with deoxyribonuclease, destroying DNA, then added to R strain • Discovery: DNA caused the transformation ...
Slide 1
Slide 1

... etc.) • How DNA is replicated ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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