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3.12 Translation
3.12 Translation

... After a protein is done being made, it needs to have a signal to stop adding on more amino acids. All ...
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没有幻灯片标题

... the duplicated state becomes visible only at the end. The molecular interactions of any individual crossing-over event involve two of the four duplex DNAs. ...
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Ch5hybridisationSNPRFLP

... • GMO = genetically modified organism, GMM = genetically modified microorganisme ...
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No Slide Title

...  The gene for ribosomal RNAs occur as repetitive sequence and together with the genes for some transfer RNAs in several thousand of copies  Structural genes are present in only a few copies, sometimes just single copy. Structural genes encoding for structurally and functionally related proteins of ...
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downloadable file

... nucleotides and an enzyme called DNA polymerase which incorporates new nucleotide bases making a new piece of DNA which is a copy of the original piece. In Sanger’s original method, four different sequencing reactions are performed. Each reaction contains a different modified nucleotide that once in ...
DNA, RNA and Protein Power Point
DNA, RNA and Protein Power Point

... 1. RNA is single stranded 2. The sugar in RNA is Ribose, not deoxyribose as in DNA 3. The DNA nucleotide thymine is replaced by the RNA nucleotide Uracil ...
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Recombinant DNA and Plasmids

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Recombinant DNA and Genetic Engineering

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Lucerne Publishing F

... corresponding to 20 letters of our alphabet. You can then use this system to ‘spell’ the parts of a protein. The code is called the ‘DNA Alias’ and each letter represents a group of 3 letters (codon). When you see the DNA Alias of a protein, you can find the original DNA sequence by reversing the co ...
Unit III: Introduction to Cells Unit IV: Cell Processes
Unit III: Introduction to Cells Unit IV: Cell Processes

... 2. Most of the time the DNA in our cells is loosely-arranged _______________. During interphase, the cell grows and chromatin ___________. Prior to dividing, the chromatin condenses forming structures known as__________________, which separate during Mitosis. 3. Discuss the role of centrioles during ...
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Spring 2005 - Antelope Valley College

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The Genetic Code
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... into a polypeptide. It must first be converted into mRNA.  The mRNA strand that is created from the DNA template is the COMPLEMENT. It differs from the DNA complement strand, as it contains Uracil (U) instead of Thymine (T) ...
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... ATRi treated SV40-infected or mock infected cells compared to SV40-infected cells in the presence of DMSO (Viability (% of DMSO), the OD of mock or SV40-infected cells in the presence/ absence of ATRi was divided by the OD generated from SV40 infected cells in the presence of DMSO. Plasmids and tran ...
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Only One Strand of DNA Is Translated

... and “late” genes read from the same strand? Jayaraman and Goldberg separated the T4 DNA into heavy and light strands, and challenged each separately with “early” mRNA and “late” mRNA. They added a DNA endonculease that degraded single-stranded DNA, so that any DNA not bound by the mRNA was degraded. ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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