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Slide 1
Slide 1

... etc.) • How DNA is replicated ...
Glossary - The Birman Cat Club
Glossary - The Birman Cat Club

... Amino acid: building block of proteins, there are 20 of these Autosome: any chromosomes other than the sex chromosomes X and Y Back cross: where a son or daughter is mated back to one of its parents or grandparents Candidate gene: a gene that is suspected of being the disease gene Chromosome: Contai ...
DNA Structure
DNA Structure

... _____________________________. Two copies of each chromosome also have two copies of the _______________. One allele may be ________________ and the other ______________________. ...
Recitation Section 7 Answer Key Molecular Biology—DNA as
Recitation Section 7 Answer Key Molecular Biology—DNA as

... Observations of heritable traits indicate that genetic information must be recorded somehow and also must be passed on to offspring. In addition, change over time indicates that there must also be a mechanism that allows the information to be changed. 3. Given what you now know about DNA structure, ...
restriction enzymes
restriction enzymes

... •BACs: bacteria artificial chromosomes insert size 100-500 kb •YACs: yeast articifical chromosomes 250-1000kb (1 mb) •cloning •generate fragments with a restriction enzyme. Sources can be whole DNA sample (genomic), or DNA generated from RNA of particular tissue •mix with linearized (restricted) pla ...
MOLECULAR CLONING OF A GENE: With Recombinant DNA
MOLECULAR CLONING OF A GENE: With Recombinant DNA

Grooving Down the Helix
Grooving Down the Helix

... protein, the rate of motion decreased much more rapidly than it would have for a simple linear motion. Relying on the same technique, the group went on to analyze the diffusion rates of eight different proteins of various sizes. These molecules had highly diverse functions — such as DNA replication, ...
Bio 111
Bio 111

... c. NAD+ is regenerated for use in glycolysis. d. The oxidation of pyruvic acid becomes possible. e. ATP is produced. ...
Lecture 8
Lecture 8

... region and added to the growing 3’-end •! Nucleotides are added according to the rules of base pairing T!A, C!G, G!C, and A!U Only a short segment RNA is bound to the template at any one time ...
2-centrioles & fibers disappear
2-centrioles & fibers disappear

... • When the tRNA matches its anticodons to the mRNA’s codons at the ribosomes, it brings with it a particular amino acid. After the tRNA’s drops off amino acids from the start to the stop codon, the protein is complete. ...
Enzymes involved in DNA replication Enzyme Role Helicase or
Enzymes involved in DNA replication Enzyme Role Helicase or

Transposons: Mobile DNA DNA
Transposons: Mobile DNA DNA

... 2. The RNA transcript is copied into DNA, which initially exists as an independent molecule outside of the genome. This conversion of RNA to DNA, the reverse of normal transcription process required reverse transcriptase. It is often coded by a gene within the transposon and is translated from the R ...
Study Guide
Study Guide

... • In general, when studying a biological event, one should try not to disrupt the event in the process of measuring it. In this particular study, you might wonder whether DNA polymerase can still function when GFP is attached to one of its subunits. This paragraph has the answer. • How did the locat ...
BIO-RAD Lambda DNA Kit, AP Bio Lab 6B, and BIO
BIO-RAD Lambda DNA Kit, AP Bio Lab 6B, and BIO

... • 11/13 at before 5th period Pour 8 gels (1%, EtBr, 10 well comb) • Prep. HindIII standard (right before class) ...
Schedule
Schedule

... DNA OR cause a mutation, which could harm the patient when it is inserted OR it could have a detrimental effect on the ...
Chapter 20 – DNA Technology - Fort Thomas Independent Schools
Chapter 20 – DNA Technology - Fort Thomas Independent Schools

... e) sugar molecules that are bound to the ends of a DNA fragment 5. One feature of “engineered” plasmids that is helpful in the isolation and analysis of cloned DNA is: a) they can only handle DNA fragments of up to 120 kb b) that they are an integral part of all eukaryotic cells c) they contain no g ...
1st
1st

... • The numbers and types of offspring in a cross are determined by the above laws • Separate genes behave independently of each other (later, exceptions to this rule were found) ...
無投影片標題
無投影片標題

... Vaccinia virus  carry smaller size of foreign genes  DNA recombination occurs in the cells  virus replicate within the cytoplasm of the host cells  higher level of protein expression ...
DNA
DNA

... Cell to Cell Communication • Chemical Signals (hormones) can be sent from one cell to another to communicate • Receptor proteins on the plasma membrane receive the signal ...
Mutation and DNA Repair
Mutation and DNA Repair

... the “p” is the connecting phosphate) gets methylated: a CH3 group is attached to the 5 position on the ring. When 5-methyl cytosine is spontaneously deaminated, it is converted to thymine, a standard DNA base. Replication leads to a base change: one daughter stays a C-G base pair while the other is ...
BIO208
BIO208

... 11. A bacterial cell has a lactose operon but the promoter is defective (mutated). All else is normal. The bacteria is transformed with a plasmid that contains a wildtype (non-mutated) promoter, amp resistance gene, and origin of replication. Can the cell utilize lactose when grown in the presence o ...
PowerPoint Presentation - Ch. 10 Molecular Biology of the Gene
PowerPoint Presentation - Ch. 10 Molecular Biology of the Gene

... Egypt, the Rosetta Stone was found, deciphering Egyptian hieroglyphics to Egyptian script to Greek. • How is this like our study of genetics? • DNA transcribed to RNA translated to a polypeptide. ...
Jeopardy, cells part 2 review
Jeopardy, cells part 2 review

... Which of the following may alter mitosis and cause mutations of DNA. A)medications B) chemical exposture C) radiation D) all of the above ...
AP Biology
AP Biology

... 31. What is the basic concept of the whole-genome shotgun approach to sequencing? ...
1 Biology 20 Protein Synthesis DNA: How is this linear information
1 Biology 20 Protein Synthesis DNA: How is this linear information

... 3 Stages of Protein Synthesis - "The process": (p. 199; Fig. 10.13B and 10.14) a) Initiation: 1) Binding of: a) b) c) 2) Large subunit attaches: b) Elongation: 1) Codon recognition: 2) Peptide bond formation: 3) Translocation: ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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