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How is coordinated DNA damage repair and control of mitotic commitment ensuring longterm genomic stability during successive cell cycles? Candidates are welcome for a Postdoctoral position at Gustave Roussy Cancer Campus, Villejuif, France. Applications for funding will be submitted to European or French agencies (EMBO fellowships…). During proliferation, all somatic cells reproducibly execute an intermediate G2 phase, of up to several hours, before mitotic commitment. This G2 phase is hypothesized to provide more time for completion of the DNA replication program and/or repair of DNA damages appearing during replication, before cell division. Entry into mitosis in mammals is tightly regulated by the active state of a MEN (Mitotic Entry Network) signaling pathway, including Aurora-A, Polo-like kinase 1 and CyclinB1-Cdk1 kinases (Gavet & Pines, 2010a, 2010b). Surprisingly, how are coordinated DDR (DNA Damage Response) and MEN signaling pathways in space and time during any cell cycle ensuring a timely entry into mitosis and contributing to the maintenance of genomic stability remain poorly understood. Previous investigations showed that conditional inactivation of Checkpoint protein 1 (Chk1) induce a premature entry into mitosis during unperturbed cell cycles, suggesting that (some) DDR pathways will control normal G2/M progression. Our main objective is to quantitatively analyze spatio-temporal regulation of DDR signaling pathways during normal G2 phase and to determine how it is coordinated with MEN activation and entry into mitosis. This project will be strongly based on real time live cell imaging assays combined with the use of FRET (Fluorescence Resonance Energy Transfer) biosensors of kinase activities (Gheghiani & Gavet, 2014). Our research group is located at the Gustave Roussy Institute, a leading Cancer Research center in Europe (~ 20min from Paris city center) in the research unit UMR8200 CNRS Genetic stability and oncogenesis. http://www.gustaveroussy.fr/fr/content/divisioncellulaire-et-stabilité-génomique. The Institute provide core facilities for live cell and intravital imaging, flow cytometry, bioinformatics, viral vector production, genomics, proteomics and animal housing. The fellow will join a stimulating environment with several groups working on different aspects of DNA repair mechanisms and signaling pathways that preserve genome integrity and prevent cancer. Applications Interested candidates are encouraged to send a CV and names of 2 referees to: Olivier GAVET GUSTAVE ROUSSY Cancer Campus Cell division and genomic stability team lab: +33 1 42 11 62 25 E-mail: [email protected]