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Assessment
Assessment

... recognizes the transcription start site of a gene? a. The polymerase strings amino acids into a polypeptide. b. Free-floating nucleotides pair up with exposed DNA bases. c. A complementary RNA strand detaches itself from the DNA. d. The DNA strand begins to unwind, separating the two strands. _____ ...
BCPS Biology Reteaching Guide Genetics Vocab Card Definitions
BCPS Biology Reteaching Guide Genetics Vocab Card Definitions

dna-structure-replication
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... Name the organic base which pairs with cytosine. Name the organic base which pairs with thymine. ...
Electrochemical DNA Biosensors
Electrochemical DNA Biosensors

... regeneration schemes have been shown useful for “removing” the bound target in connection with different DNA biosensor formats. • Even more elegant is the use of controlled electric fields for facilitating the denaturation of the duplex ...
Unti 8-9 - DNA, RNA, and Protein Synthesis
Unti 8-9 - DNA, RNA, and Protein Synthesis

... Score 4: Student demonstrates in-depth inferences and applications of the learning goal(s) and can reconstruct and apply their knowledge from limited information: A/B4) Describe important discoveries that led to today’s model of DNA structure and explain how the development of the DNA model exhibits ...
Protein Synthesis: A Real Adventure
Protein Synthesis: A Real Adventure

... 1 The mRNA student will enter the nucleus and transcribe the DNA into mRNA. REMEMBER, THE DNA CANNOT LEAVE THE NUCLEUS! 2. The mRNA student takes the mRNA to the Ribosome (your desk).Each set of three letters represents a codon. 3. The tRNA student will search out the correct anti-codon sequence car ...
Molecular Pathology - Charles River Laboratories
Molecular Pathology - Charles River Laboratories

... By combining a strong history in molecular biology and histopathology, Charles River can relate gene expression to tissue histomorphology in both normal tissues and therapeutic models of disease, providing you with that valuable functional genomics information. The end result is the best possible in ...
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student notes protein synthesis mutation

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Ligation and Transformation
Ligation and Transformation

... 4. Transformed cells are grown on selection media ...
Genetic engineering
Genetic engineering

... Step 2: Cut it out of the chromosome (in daffodil) using restriction enzymes. Restrictions enzymes are bacterial proteins that have the ability to cut both strands of the DNA molecule at a specific nucleotide sequence Resulting fragments can have blunt ends or sticky ends ...
RNA and Protein
RNA and Protein

... 2. Frameshift Mutations – result from the insertion or deletion of bases which cause a shift in the frame of the amino acid sequence. Insertion – a gain of a nucleotide in the DNA sequence. ...
Chapter 12: Genetic Engineering
Chapter 12: Genetic Engineering

... DNA Recombination ...
Biology: Unit 13 Directed Reading Guide
Biology: Unit 13 Directed Reading Guide

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Genetics Study Guide Answers What are different forms of a

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Practical molecular biology
Practical molecular biology

... Type I enzymes cut at a site that differs, and is located at least at at least 1000 bp away, from their recognition site. Type II enzymes recognize sites of 4-8 nucleotides and cleave DNA at the same site ...
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Conjugative plasmids are circular pieces of DNA that not only

... In Figure 1B, why is it important that the authors chose to make silent mutations in the region of nes? Why do the authors focus exclusively on spacer region 1, what do you think is in the other two spacers? 2. In figure 1, conjugation experiments were performed by mixing an S. epidermidis donor str ...
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Sample Exam #2 ( file)
Sample Exam #2 ( file)

... A single deletion of base in coding sequence results in _____. A. transcription termination B. a frameshift mutation C. missense mutation D. nonsense mutation ...
Banana DNA Extraction Lab
Banana DNA Extraction Lab

... The process of isolating DNA from a cell is the first step of many laboratory procedures in biotechnology. The scientist must be able to separate the DNA from the unwanted substances of the cell gently enough so that the DNA is not broken up and sheared. A “filtrate” is made of bananas and treated w ...
Biology Recitation 07.07.2010
Biology Recitation 07.07.2010

... Protein folding. We reviewed the chemical properties of individual amino acids, introduced their acidity (pKa), hydrophobicity and affinity for each other. Don’t be confused, this topic has consumed many scientists’ entire lives and the treatment of it today was greatly simplified. The take home mes ...
GROUP 4 XERODERMA PIGMENTOSUM INTRODUCTION Xeroderma pigmentosum
GROUP 4 XERODERMA PIGMENTOSUM INTRODUCTION Xeroderma pigmentosum

...  The diagnosis of XP is made on the basis of clinical findings and family history  The diagnosis of XP is based on skin, eye, and nervous system  XP can be diagnosed by measuring the DNA repair factor from skin or blood sample ...
Unit VII: Genetics
Unit VII: Genetics

DNA Structure and Function
DNA Structure and Function

... Release factor (enzyme) cleaves polypeptide from last tRNA which then leaves P site. Subunits dissociate. ...
Inheritance matching
Inheritance matching

... regarding a particular characteristic, e.g, Ff, ff. ...
DNA unit Summary
DNA unit Summary

... are held together by weak hydrogen bonds between the nitrogen-containing bases. The sides of the ladder consist of phosphate groups alternating with a five-carbon sugar. In DNA, deoxyribose is the 5-carbon sugar. The hydrogen bonding allows for only certain base pairings. In DNA, adenine will bond w ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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