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IB Biology--Chromosome Review Activity
IB Biology--Chromosome Review Activity

Glossary of Biotechnology Terms
Glossary of Biotechnology Terms

... same phenotype. Many cancers, as well as other genetic diseases like type-I diabetes, are genetically heterogeneous. Note that this term is used when different genetic defects can independently cause disease. When multiple defective genes must combine to cause a disease, we say that they act in epis ...
Biology 303 EXAM II 3/14/00 NAME
Biology 303 EXAM II 3/14/00 NAME

... Such individuals therefore have 47 chromosomes. While there is impaired fertility of both sexes, females are more likely to be fertile than males. Assume that children are born to a female with Down syndrome and a normal 46-chromosome male. What proportion of the offspring would be expected to have ...
Rna guided notes
Rna guided notes

Chapter 6, Section 3: Advances in Genetics
Chapter 6, Section 3: Advances in Genetics

... cows so that the cows produce the protein responsible for blood clotting in their milk. The protein is then extracted and used to treat hemophiliacs.  Genes have also been inserted into plant DNA which enables plants to survive in cold temperatures or in poor soil. Some genetically engineered plant ...
No Slide Title
No Slide Title

... 21. An ultracentrifuge consists of a rotor that spins tubes containing materials and is: (A) a component on a new type of microscope to allow cell components to be easily visualized (B) the laboratory tool developed by Robert Hooke in the 1660s that he used to discover cells (C) a tool used by cell ...
The Quest for Ancient DNA
The Quest for Ancient DNA

... DNA From Other Sources Can Give Clues About Human Migration The Story of Human Migration Also is Told in the DNA of Parasites and Pets. ...
dnaprotein synthesis
dnaprotein synthesis

... of nucleotides inserted or deleted is not a multiple of three 1. addition or deletion can result in a frameshift 2. results in a completely different sequence of amino acids in the polypeptide chain ...
Genetic Engineering and Biotechnology
Genetic Engineering and Biotechnology

... Stem Cell Research (we will come back to this one later) Gene Therapy DNA Fingerprinting (and other Forensics applications) ...
Chapter 13 - Angelfire
Chapter 13 - Angelfire

... – Ex: a protein only cuts at AATT, it will cut the two fragments at different points - not across from each other (called sticky ends) • Called sticky ends because they want to bond with things due to their “open” end ...
Bio 220 MiniQuiz 1
Bio 220 MiniQuiz 1

... _____1. Organic growth factors include amino acids and vitamins. _____2. Chemoautotrophs use the light from the sun as their primary energy source. _____3. Both chocolate agar and blood agar contain blood. _____4. Transcription refers to the process of DNA synthesis. Multiple choice _____5. An oblig ...
Biology - The Roblesite
Biology - The Roblesite

... ________________, which lets the enzyme recognize the start of a gene. 13. When mRNA is being assembled, it grows in the ________to __________direction. 14. These numbers are based on the position of ____________atoms in the ________________molecules, which, along with phosphate groups, comprise the ...
Model 1 Q 1:cartilaginous joint Q2: deoxribose sugar and phosphate
Model 1 Q 1:cartilaginous joint Q2: deoxribose sugar and phosphate

... Q12: increase re absorption of sodium Q13:* To get entire genome of cell by broken up DNA at it ,then cleaved with restriction endonuclease, these DNA piece are spliced into plasmids or phages and cloned, then various selective techniques are used to isolate the desired DNA sequences. *it starts wit ...
BP 32: Posters - DNA/RNA - DPG
BP 32: Posters - DNA/RNA - DPG

... high concentration and low concentration regions. Transcription of DNA is carried out by RNA Polymerase II (Pol II) in low DNA density regions. While this organization reflects a need to unfold DNA for Pol II access, the causal origin of this spatial organization remains unclear. Here, we investigat ...
Lecture 9
Lecture 9

... – Mutagenic substance may cause new mutation that reverse the original mutation to his+ ( back mutation or reversions) – Incubation with mutagen / Control – without mutagen – Liver extract – supply all necessary activation enzymes ...
Lab 4 Restriction Analysis
Lab 4 Restriction Analysis

... containing thousands of genes. The only way to break it into smaller segments was to physically shear it. But these fragments would be random, not reproducible, and were rapidly degraded by cellular nucleases if reintroduced into the cell. RE's, for the first time, allowed scientists to cut DNA into ...
Genetic Mutations - Velma Jackson High
Genetic Mutations - Velma Jackson High

... noises. As the disease progresses, children with Tay-Sachs disease experience seizures, vision and hearing loss, intellectual disability, and paralysis. An eye abnormality called a cherry-red spot, which can be identified with an eye examination, is characteristic of this disorder. Children with thi ...
Central Dogma Activity Worksheet
Central Dogma Activity Worksheet

... construct a house, the DNA "blueprint" tells the cell how to build the organism. Yet, how can a heart be so different from a brain if all the cells contain the same instructions? A cell has the ability to turn off most genes and only work with the genes necessary to do a job. Remember, a gene is sec ...
Regulation of Gene Expression – Part III
Regulation of Gene Expression – Part III

... • ____________________– change in a ________ DNA nucleotide, and, therefore, possible change in a specific amino acid ex. What results in the cell sickled red blood cell • ____________________– occur most often when 1 or more nucleotides are either a) inserted or b) deleted from DNA. Result: a compl ...
Restriction Enzymes, Gel Electrophoresis and Mapping DNA
Restriction Enzymes, Gel Electrophoresis and Mapping DNA

... • Combine mutant viral DNA with restriction fragment of wt virus • If restriction fragment can complement mutation, site of mutation must lie within fragment position on map • Depends on homologous recombination ...
Genetics Part 2B 2015
Genetics Part 2B 2015

... 6. Agricultural applications - GMOs ...
Plasmids, primers (and beyond!)
Plasmids, primers (and beyond!)

... enzymes. Restriction enzymes recognize and cleave certain palindromic pieces of DNA. ...
M0302Datasheet-Lot0021309
M0302Datasheet-Lot0021309

DNA Technology
DNA Technology

... 2. All RFLP’s from sample inserted into a well in a gel 3. An electrical charge is applied to the gel, which causes RFLP’s to migrate down gel according to length (how? chromatography), resulting in a distinct pattern of bands based on genetic code. 4. Gel’s have many wells so that different samples ...
Microbe Diversity
Microbe Diversity

... Archaea (prokaryotes, but with similarities to next group) Eukarya (eukaryotes – have DNA in a nucleus) › Protista – single celled eukaryotes  Many different ways of feeding and living  Some are plant-like, some animal-like, and some fungal-like › Metazoans – multi-cellular eukaryotes ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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