• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Sem2 Final SG 12 Part1
Sem2 Final SG 12 Part1

... Biotechnology 13. What are some benefits of genetic engineering? 14. How would you make a recombinant DNA? 15. Describe how viruses reproduce. 16. Describe how each of these is used in biotechnology: cloning, gel electrophoresis, gene therapy, recombinant plasmid, transgenic/genetically modified org ...
Human Genome Project
Human Genome Project

... chromosomes (3 billion bases) – Identify genes within those sequences (~30 000 genes) – Locate the position of the genes on the chromosomes ...
Do Now: - South Orange
Do Now: - South Orange

... Do Now: •Define genotype and phenotype. Then determine the relationship between the two. ...
A1979HZ32700001
A1979HZ32700001

... elucidate the mechanism of the nuclear staining reaction. We were then able to publish the first cytochemical method for the direct visualization of histones (and related basic proteins), explain how it worked and provide some quantitative data, relating DNA to histone content of nuclei. "Since then ...
Dominant trait - Integrated Science 3
Dominant trait - Integrated Science 3

... Stronger trait, only need to have one copy The information storage of a cell Version of a type of gene The twisted stairway shape of DNA Permanent change in the DNA, through alteration of sequences Enzyme used to cut DNA Long pieces of DNA which contains genes DNA from two different species, recombi ...
Topic 7.1
Topic 7.1

LETTER Insertion DNA Promotes Ectopic Recombination during
LETTER Insertion DNA Promotes Ectopic Recombination during

... that they were true recombinants, which contained only 1 copy of 2 fragments of GUS gene. The results were also supported by the fact that their selfed progenies segregate at roughly a 3:1 ratio, expected from the plants with fully stained leaves. Additionally, the stained patterns on leaves and the ...
recombinant dna technology
recombinant dna technology

... • FIRST, THE PLASMID IS TREATED WITH THE SAME RESTRICTION ENZYME AS WAS USED TO CREATE THE DNA FRAGMENT • THE RESTRICTION ENZYME WILL CUT THE PLASMID AT THE SAME RECOGNITION SEQUENCES, PRODUCING THE SAME STICKY ENDS CARRIED BY THE FRAGMENTS • MIXING THE FRAGMENTS WITH THE CUT PLASMIDS ALLOWS BASE-PA ...
College Prep: Review
College Prep: Review

... 15. List THREE differences between DNA structure vs. RNA structure. 1 DNA is large, RNA is small 2. DNA is double stranded, RNA is single 3. DNA has thymine, RNA has uracil 4. DNA is helical, RNA is not 5. DNA has 1 type, RNA has 3 mRNA tRNA rRNA 16. What type of macromolecule are DNA and RNA? Nucl ...
Lab Business - Memorial University
Lab Business - Memorial University

... SCOTUS in essence recognized that BRCA genes are ‘products of nature’ and thus on accepted principles not patentable. Such genes include expressed exon and intervening intron regions, as well as upstream and downstream promoters, enhancers, and other paraphernalia by which genes get things done. The ...
DNA Technology
DNA Technology

... Introduction  Since the 1970’s, humans have been attempted to manipulate and modify genes in a way that was somewhat predictable.  Biotechnology merges biological information with computer technology to advance research.  Biotechnology involves techniques that are used to make or modify the prod ...
DNA paper 1 - DavidHein-CESRC-page
DNA paper 1 - DavidHein-CESRC-page

... Genes are play a major role in who you are. Each person has two copies of each gene. Genes are segments of DNA that contain the coding for making polypeptides or protein. The Human Genome project estimates that humans have between 20,000 and 25,000 genes. Some genes are called housekeeping genes. Th ...
Genetic Engineering
Genetic Engineering

... A strand of DNA formed by the spicing of DNA from two different species is called? Recombinant DNA Recombinant DNA is formed by joining DNA molecules from two different species. Cleaving DNA with a restriction enzymes. Recombining pieces of DNA from different species, cloning and screening target ce ...
No Slide Title
No Slide Title

... order of alleles (“haplotype”) is conserved in uninterrupted blocks or “neighborhoods” that tend to be inherited together, with recombination occurring between them Most population share common SNP variants and haplotypes Haplotype blocks and the tag SNPs that identify them will form the HapMap ...
Chapter 20 Terms to Know
Chapter 20 Terms to Know

... Insulin is made by the pancreas.  This peptide hormone (protein) ensures that glucose is taken up by the ...
genetic engineering 2 - Hicksville Public Schools
genetic engineering 2 - Hicksville Public Schools

dna review - NVHSIntroBioPiper1
dna review - NVHSIntroBioPiper1

... Thymine and Guanine always pairs with Cytosine. ...
BCM301 Food Biotechnology
BCM301 Food Biotechnology

... structural genes that maintain routine (household) cellular functions • Cells express other specialised genes which give the cells there unique properties ...
37. Recombinant Protocol and Results-TEACHER
37. Recombinant Protocol and Results-TEACHER

... Sticky Ends: The ends of double-stranded DNA fragments that are easily paired with complementary bases on other DNA molecules. Plasmid: Small, circular, double-stranded DNA molecules that are different from a cell’s chromosomal DNA. Transformation: The process by which the recombinant DNA is inserte ...
Crash Course Biology Notes on: DNA Structure and Replication
Crash Course Biology Notes on: DNA Structure and Replication

... Crash Course Biology Notes on: DNA Structure and Replication 15. What part of the DNA molecule holds the genetic coding that makes you, you? 16. What are the four nitrogenous bases? 17. Is DNA in living organisms a single polynucleotide molecule or a pair of polynucleotide molecules held together? ...
DNA (Deoxyribonucleic Acid)
DNA (Deoxyribonucleic Acid)

... 1. Why is transcription necessary? Transcription makes messenger RNA (MRNA) to carry the code for proteins out of the nucleus to the ribosomes in the cytoplasm. 2. Describe transcription. RNA polymerase binds to DNA, separates the strands, then uses one strand as a template to assemble MRNA. 3. Why ...
DNA Recap
DNA Recap

... H. The  process  by  which  DNA  is  copied  into  an  RNA  molecule   I. A  molecule  that  is  made  of  amino  acids  and  performs  ALL  important   roles  –  structure,  function,  and  regulation  –  of  cells  and  organisms ...
notes - QuarkPhysics.ca
notes - QuarkPhysics.ca

... structures. Once an initiator protein locates the correct place to begin copying, a helicase “unzipper” unwinds the strands at approximately 8000 rpm, forming a fork area, without tangling the DNA strands as they separate. An “untwister” enzyme (topo-isomerase) systematically cuts and repairs result ...
Green Fluorescent Protein (GFP)
Green Fluorescent Protein (GFP)

... – human colon (large intestines) – 2 x 1010 (billion) new E. coli each day! – spontaneous mutations • for 1 gene, only ~1 mutation in 10 million replications • each day, ~2,000 bacteria develop mutation in that gene • but consider all 4300 genes, then: 4300 x 2000 = 9 million mutations per day per h ...
PowerPoint
PowerPoint

... The pollen from the genetically modified plants is also contaminated. When this pollen is around other plants, even things like grass or weeds, they cross pollinate. This could develop “superweeds” that have the same resistance properties as the crops. 2. Allergies On The Rise Ever since the introdu ...
< 1 ... 626 627 628 629 630 631 632 633 634 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report