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Profile Documents Logout
Upload
this certificate as PDF
this certificate as PDF

... Certificate updated July 01, 2014 The authenticity and updates of this certificate can be verified on http://www.pigen.be This certificate1 ensures there exists a DNA PROFILE of the pigeon with ringnumber ...
Double-Strand Specific DNase (dsDNase)
Double-Strand Specific DNase (dsDNase)

... Properties dsDNase is an endonuclease that cleaves phosphodiester linkages in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. dsDNase has a very high specific activity, estimated 30 times higher than bovine DNase I, and it is heat labile. dsDNase has a particularly strong pr ...
Slide 1
Slide 1

... and back ...
PCR amplifies any target DNA sequence. (N)
PCR amplifies any target DNA sequence. (N)

... Gene replacement in mice -- germline incorporation ...
Extracting DNA from Eukayotic Cells
Extracting DNA from Eukayotic Cells

... DNA is a nucleic acid found in the nucleus of cells that stores and transmits genetic information from one generation of an organism to the next by coding for the production of a cell’s proteins. Bacteria, fungi, plants, animals and all other living organisms on this planet contain nucleic acids. Th ...
Keystone Review Module B
Keystone Review Module B

... 4. The flounder is a species of fish that can live in very cold water. The fish produces an “antifreeze” protein that prevents ice crystals from forming in its blood. The DNA for this protein has been identified. An enzyme is used to cut and remove this section of flounder DNA that is then spliced i ...
DNA- (Deoxyribonucleic acid)- genetic material that carries the
DNA- (Deoxyribonucleic acid)- genetic material that carries the

... DNA are made up four different nitrogen bases pairs. Adenine (A), Thymine (T), Guanine (G), Cytosine (C) are the nitrogen bases. Adenine always pairs with Thymine and Guanine always pairs with Cytosine. With these four base pairs, there are 8,000,000 possible outcomes between two parents and the arr ...
DNA - pupul.ir pupuol
DNA - pupul.ir pupuol

... • RNA can be hydrolyzed by alkali to 2′,3′ cyclic diesters of the mononucleotides, • compounds that cannot be formed from alkali-treated DNA because of the absence of a 2′-hydroxyl group. • The alkali liability of RNA is ...
App1PCR - FSU Biology
App1PCR - FSU Biology

... The polymerase chain reaction, or PCR, is a technique that allows for the amplification of a specific target DNA sequence within a larger population of DNA (such as the human genome). Using PCR, picogram quantities of target DNA can be amplified to yield microgram quantities for subsequent biochemic ...
013368718X_CH12_179-192.indd
013368718X_CH12_179-192.indd

... reconstruct the other half by the mechanism of base pairing. Because each strand can be used to make the other strand, the strands are said to be complementary. DNA copies itself through the process of replication: The two strands of the double helix unzip, forming replication forks. New bases are a ...
Transcription, Translation, and Protein Synthesis
Transcription, Translation, and Protein Synthesis

... 1. Assemble groups of three and within each group decide on one person for each of the following roles: RNA polymerase, tRNA, and ribosome (these roles should be changed for each protein constructed). 2. The RNA polymerase must go up to the “nucleus” (the front desk) and choose one of the DNA ...
Lecture 19-Chap15
Lecture 19-Chap15

... complex has a tetramer of Cre recombinases, with one enzyme monomer bound to each half site. ...
notes Protein_Synthe.. - hrsbstaff.ednet.ns.ca
notes Protein_Synthe.. - hrsbstaff.ednet.ns.ca

... 5.3 Transcription and Translation How does DNA control a cell? This control is through protein synthesis. Protein Synthesis requires RNA. DNA Sugar Structure Type of sugar Nitrogenous Bases # of strands ...
Genetic Engineering - Biology Class With Mrs. Caskey
Genetic Engineering - Biology Class With Mrs. Caskey

... • A specific gene has been added to the cats’ DNA which causes them to glow in the dark. • Normally, the gene is found in ...
File - cOACH RICH`S BIOLOGY CLASS
File - cOACH RICH`S BIOLOGY CLASS

... both directions until the chromosome is replicated ...
PCR - churchillcollegebiblio
PCR - churchillcollegebiblio

... forward primer and one is a reverse primer. When they have bound to the complementary sequences on the genomic DNA template strand, they show the Taq polymerase where to start DNA synthesis. The primers are responsible for making sure that only the region of interest is copied. • This is double-stra ...
Biotechnology
Biotechnology

... By definition, __________ is the DNA Cloning production of genetically __________ copies of DNA, cells, or organisms through some __________ means. Ex.: when a plant sends up an underground stem or a root sends up a new shoot, the resulting plant is a clone of the original plant. Ex.: members of a b ...
Spring Semester Exam Study Guide- Biology Every cell contains
Spring Semester Exam Study Guide- Biology Every cell contains

... scientist sequences homologous genes in several different related species. To find out which two species are most closely related, how should the scientist analyze the data? a. Count the number of amino acid differences c. Count the total number of amino acids in the between sequences from different ...
The Nucleus, Chromosomes and Genes
The Nucleus, Chromosomes and Genes

Central Dogma of Cell Biology
Central Dogma of Cell Biology

... • The mRNA leaves the nucleus  cytoplasm • Message is read at the ribosome • 1 Codon (3 letter message) is translated into 1 amino acid • tRNA molecule has one end (anticodon) that matches the mRNA . Each anticodon specifies an amino acid. • The amino acids are bonded together as peptide chains…whi ...
Sequencing a genome - Information Services and Technology
Sequencing a genome - Information Services and Technology

... • Loop of bacterial DNA that replicates independently of the chromosomes; artificial plasmids can be inserted into bacteria to amplify DNA for sequencing ...
TAKS Review - SchoolNotes
TAKS Review - SchoolNotes

... A Translation of DNA into amino acids B Separation of the DNA molecule into codons C Transformation of DNA into RNA D Separation of the DNA double helix ...
No Slide Title
No Slide Title

... Figure 2. Occurrence of somatic mutation in one DNA strand in the G1 phase of the cell cycle. Somatic mutation was induced in BL2 cells in the G1 phase of the cell cycle. Single cells were either analyzed for mutations in the V4-39 gene after 90 min of stimulation or isolated in single wells and l ...
Protein Synthesis Practice
Protein Synthesis Practice

... Given the following mRNA strands, draw a circle around the START CODONS and the STOP CODONS. Number the 3-base pair codons in between. A whole protein need to have a series of codons between a start (AUG) and a stop codon (UGA, UAG or UAA). Which strands will build whole proteins? Example: This str ...
chapter18-20packet
chapter18-20packet

... b. Give an example of heterochromatin common in mammalian cells. ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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