• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Título 01 Universidade Fernando Pessoa
Título 01 Universidade Fernando Pessoa

... • Quick, highly redundant – requires 7-9X coverage for sequencing reads of 500-750bp. This means that for the Human Genome of 3 billion bp, 21-27 billion bases need to be sequence to provide adequate fragment overlap. • Computationally intensive • Troubles with repetitive DNA • Original strategy of ...
chapter18-20packet
chapter18-20packet

... b. Give an example of heterochromatin common in mammalian cells. ...
BIO 304 Genetics
BIO 304 Genetics

... Use one of the above terms to best complete each sentence #1-15 below. (2 pts. each) 1. Purine bases commonly found in DNA are guanine and ____adenine_____. 2. The normal phenotype that is typical of most individuals in a population is called __wild type___ . 3. A mutation of an enzyme-encoding gene ...
Deoxyribonucleic Acid (DNA)
Deoxyribonucleic Acid (DNA)

... Each DNA strand is made of combinations of four chemical units, called nucleotide bases, which comprise the genetic "alphabet." The bases are adenine (A), thymine (T), guanine (G), and cytosine (C). Bases on opposite strands pair specifically: A’s always pair with T’s, and C’s always pair with G’s. ...
Cell Division and Mitosis
Cell Division and Mitosis

... alternating sets of phosphate and sugar molecules. One side is 5’-3’ and the other side is 3’ - 5’.  Base pairs make up the rungs of the ladder A—T and G– C. The genetic code is arranged as sets of three base codes together. For example AAA, CGC, ATC, CCC, would all code for four different amino ...
Genetic Engineering Notes 2017
Genetic Engineering Notes 2017

... Polymerase chain reaction (PCR) is a technique that allows biologists to make copies of genes.  Small amounts of DNA can be multiplied making it easier to analyze. ...
DNA Basics - Thermo Fisher Scientific
DNA Basics - Thermo Fisher Scientific

... repel each other, just like the identical poles of two magnets will repel each other. An A won’t pair with a C, and a T won’t pair with a G. So if there’s even a single base that’s not complementary to its partner, it could keep a single strand from sticking to another single strand. What’s a gene a ...
File - Science with Mr Thompson
File - Science with Mr Thompson

... the nucleic acid RNA. These RNA copies can then used to direct protein synthesis, but they can also be used directly as parts of ribosomes or spliceosomes. ...
Human DNA Dance - University of Wisconsin Biotechnology Center
Human DNA Dance - University of Wisconsin Biotechnology Center

... asking the two lines to release their handshakes and take one step to the left, while keeping their right hands in the C, T, G or A form. You can show how two complementary single strands of DNA can anneal (come together) by then having the two strands come back together into a double-stranded form. ...
Deoxyribonucleic Acid (DNA)
Deoxyribonucleic Acid (DNA)

... Each DNA strand is made of combinations of four chemical units, called nucleotide bases, which comprise the genetic "alphabet." The bases are adenine (A), thymine (T), guanine (G), and cytosine (C). Bases on opposite strands pair specifically: A’s always pair with T’s, and C’s always pair with G’s. ...
I. Exam Section I Fundamental Cell Theory and Taxonomy (Chapter
I. Exam Section I Fundamental Cell Theory and Taxonomy (Chapter

... b. More than 200 gene families are common to all three domains c. The function of a gene can often be deducted from its sequence C. Introduction to Multicellularity (Chapter 19) 1. Regulation of Organism Size by Cell Number a. Size of cells is relatively common across species b. Number of cells dete ...
File
File

... I encourage you to work in groups, however, please write the answers down yourself. Once you have the study guide completed, I suggest that you make flashcards, or find another way to review the material on your own. Please also make a plan to study in a group. I will give you 2 points extra credit ...
File
File

... DNA Fingerprinting Activity Introduction: DNA fingerprinting relies on the fact that the DNA code is universal for all living things and that there are differences between individuals within that code. Because human DNA is very similar to every other human’s DNA, DNA fingerprinting primarily focuses ...
14 Diversity of BCR BA
14 Diversity of BCR BA

... Protein ...
Document
Document

... water to cells. 2) Discuss active vs. passive transport, diffusion, osmosis, and semipermeable membranes. 3) Given solution concentrations and different types of cells, students should be able to predict any changes that may or may not occur. What are enzymes? What are enzymes used for? How can you ...
Review questions to go with the powerpoint
Review questions to go with the powerpoint

... 55.The Human Genome Project ______________ all of human ______. This information has been used for ________ therapy. 56.DNA put together from 2 different species is called _________________ DNA. 57.A ________ is an organism made from one cell of another organism and is a genetically ______________ c ...
Haploid (__)
Haploid (__)

... Errors in the genes occur 1 for every ____________ nucleotides read Causes of ERRORS ...
Old Exam 2
Old Exam 2

... ----------------------------12. Say you were studying the genetics of a newly discovered species, Intel degradus, and you found 6 genes scattered around the organism's linear chromosome which allowed the organism to breakdown silicon. Further study revealed that all 6 genes were under the control of ...
PCR amplifies any target DNA sequence. (N)
PCR amplifies any target DNA sequence. (N)

... DNA methods summary 1. Restriction enzymes cut at specific DNA sites. (N) 2. Vectors allow genes to be “cloned” and proteins “expressed”. (N) 3. Gel electrophoresis separates DNA on the basis of size. 4. DNAs can be synthesized (up to ~100 bases commercially). (N) 5. PCR amplifies any target DNA se ...
Multiple choice questions
Multiple choice questions

... DNA-binding proteins Are usually monomeric Interact with DNA by ionic bonds Contain DNA-binding motifs Can regulate gene expression Can be isolated by affinity chromatography ...
IGEM BOOT CAMP
IGEM BOOT CAMP

... in which the genetic material carried by an individual cell is altered by incorporation of foreign (exogenous) DNA. This foreign DNA may be derived from unrelated species and even other kingdoms, such as bacteria, fungi, plants or animals, which would otherwise be inaccessible to an organism. ...
Document
Document

... If recombination rate is high, genes are far apart. ...
Genetics of bacteria and bacteriophages
Genetics of bacteria and bacteriophages

... If recombination rate is high, genes are far apart. ...
Mutations
Mutations

... DNA sequence that is very similar to that of a proteinencoding gene Sometimes transcribed but not translated May interfere with the normal gene, especially during crossing over ...
Unit 5 Vocabulary List 2 Sexual reproduction
Unit 5 Vocabulary List 2 Sexual reproduction

... different from the parent organism. Fertilization- the uniting of the sperm cell and egg cell. Genes- pieces of DNA that carry all the information passed from parents to their offspring. DNA- deoxyribonucleic acid; the genetic material of all organisms; made up of two twisted strands of sugar-phosph ...
< 1 ... 631 632 633 634 635 636 637 638 639 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report