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Name Class Date Skills Worksheet Look
Name Class Date Skills Worksheet Look

DNA and RNA
DNA and RNA

... – Most eukaryotic genes are controlled individually and have regulatory sequences that are much more complex than those of the lac operon ...
Exam 2 Worksheet part 1 KEY
Exam 2 Worksheet part 1 KEY

... DNA replication begins at an origin of replication where the two strands of the double helix are locally separated. This creates two “forks” where the double helix is unwound to form two single strands. The figure shows one replicative fork. Note, because of the antiparallel nature of the DNA double ...
DNA, RNA, and Protein
DNA, RNA, and Protein

... • DNA unwinds, at replication fork, via helicase • DNA polymerase makes 2 copies of DNA – Complementary base pairing: A=T, G=C • A & G are purines; T & C are pyrimidines • Purines are double rings; pyrimidines are single ...
Title:  P.I.’s :
Title: P.I.’s :

... Title: Epigenetics in a marine fish: Role of DNA methyltransferases in embryonic development P.I.’s : Neel Aluru and Sibel Karchner Animals have the capacity to express a variety of morphological and behavioral phenotypes under different environmental conditions. Genetic differences determine much o ...
Southern Blotting
Southern Blotting

... • Such variations include: ABO blood type, Rhesus factor, and major histocompatibility complex (MHC). • RFLP are differences in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA. • Moreover, RFLP is a sequence of DNA that has a ...
Benchmark 1st 9 weeks st guide with answers
Benchmark 1st 9 weeks st guide with answers

... how they interact with each other and their population growth environment. ...
Day 9: DNA Powerpoint
Day 9: DNA Powerpoint

... used for enzymes (proteins that make chemical reactions happen faster) ...
In 1953 Watson and Crick developed a double helix model for DNA
In 1953 Watson and Crick developed a double helix model for DNA

... The two nucleic acid strands are _______________________ to each other. That means that one strand is “_____________________” compared to the other. The 2 nucleic acid strands are held together by ____________ _ _____________ between the nitrogen bases. When the nitrogen bases bond together they fol ...
File - Biology with Radjewski
File - Biology with Radjewski

... AP Biology Radjewski 2014 ...
Biology with Junk: Protein Synthesis and Words
Biology with Junk: Protein Synthesis and Words

... 1. Make up all the DNA Template Cards and the Anti-Codon/word cards 2. Hang up the Ant-codon word cards, so the anti-codons are showing. 3. Show the students the cards and tell them what they are. 4. Tell the students that your desk is the nucleus and the DNA templates cannot leave the area. 5. A st ...
AP Biology-2nd Trimester Review Guide
AP Biology-2nd Trimester Review Guide

... independent assortment, law of segregation of alleles 17. Types of inheritance- how each works and be familiar with common examples of each: Codominance, multiple alleles, plieotropy, polygenic inheritance, sex-linked, incomplete dominance. DNA, RNA & Protein Synthesis – Chapters 16 & 17 18. DNA & R ...
Reproductive cloning
Reproductive cloning

... ●Embryonic stem cells are currently derived from extra human blastocysts that sometimes result from in vitro fertilization techniques. From 2001 to 2009, stem cell research supported by U.S. agencies was restricted to only a small number of stem cell lines. (Meanwhile, other countries were going ahe ...
DNA Study Guide CP2015
DNA Study Guide CP2015

... 1. This type of chromosomal mutation occurs when there is an unequal exchange between homologous sets and some genes are doubled. 2. This type of chromosomal mutation occurs when non-homologous sets exchange pieces. EX: genes from set 17 are on set one. 3. This type of chromosomal mutation occurs wh ...
RODENT GENOTYPING The proper identification of transgenic
RODENT GENOTYPING The proper identification of transgenic

... be obtained by a variety of techniques that vary in terms of the discomfort the animal may experience. When animals are marked by ear punches or notches, sufficient tissue can be saved to do the PCR. If the animals are to be marked this way anyway, then this technique adds no additional discomfort t ...
Biodosimetry - Arkansas State University
Biodosimetry - Arkansas State University

... – DNA has been copied, so sister chromatid is available. – Recombination event uses same (or similar DNA) – May result in wrong section being used: mutation. ...
PURINE COMPOUNDS Both the pyrimidine bases (uracil, cytosine), and
PURINE COMPOUNDS Both the pyrimidine bases (uracil, cytosine), and

... 5-FU is used for the treatment of many malignancies: breast, head and neck, adrenal, pancreatic, gastric, colon, rectal, esophageal, liver and G-U (bladder, penile, vulva, prostate) . 5-FU may be administered by bolus IV infusion or continuous IV infusion over two days every 2-3 weeks or by oral ing ...
Top 102 Biology Review
Top 102 Biology Review

... 55.The Human Genome Project ______________ all of human ______. This information has been used for ________ therapy. 56.What technique can separate DNA molecules of different length based on the size of the molecules? 57.DNA put together from 2 different species is called _________________ DNA. 58.A ...
Microbial Genetics
Microbial Genetics

... 3 base code - 4 bases ( A,U,G,C ) 64 possible combinations ( 43) Amino acids are coded for by more than one codon Genetic Code is Degenerative Genetic Code is Universal ...
Manipulating and Analyzing DNA
Manipulating and Analyzing DNA

... recombinant DNA. You will use two different websites to understand both topics. By the end of today you should be able answer the flooring questions: What are restriction enzymes? How and why are they used in biotechnology? How do restriction enzymes play a role in recombinant DNA? Restriction Enzym ...
Mitosis vs. binary fission
Mitosis vs. binary fission

... SPI 3210.1.2 Distinguish between prokaryotic and eukaryotic cells CLE 3210.1.4 Describe the processes of cell growth and reproduction. ...
BY2208 SF Genetics Central Dogma McConnell_1.1
BY2208 SF Genetics Central Dogma McConnell_1.1

... duplicate itself and control the development of the rest of the cell in a specific way.” ...
Document
Document

... The Human Genome Project was a 13-year international effort to sequence all 3 billion base pairs in human DNA and identify all human genes. The project was completed in 2003. The researchers identified markers in widely separated strands of DNA. They used “shotgun sequencing,” which uses a computer ...
Name AP EXAM REVIEW SESSION II ASSESSMENT QUIZ Use the
Name AP EXAM REVIEW SESSION II ASSESSMENT QUIZ Use the

... d. Sample 2 was cut at more restriction sites than was sample 4. e. Sample 4 was cut at more restriction sites than was sample 2. 6. Once a plasmid has incorporated specific genes, such as the gene coding for ampicillin resistance, the plasmid may be cloned by a. inserting it into a virus to generat ...
Rate of evolution
Rate of evolution

... are adapting to changes in seasonal timing. Field mustard (Brassica rapa) has evolved in response to an extreme drought in southern California, which some sources have linked to global warming. These plants flower and produce seeds near the end of the rainy season, but when the rainy season is cut s ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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