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Lecture 3 - Transcription (student)
Lecture 3 - Transcription (student)

DNA
DNA

... conservative theory. The experiment was repeated but this time the bacteria was able to replicate twice. If replication was dispersive, only one band would be expected because all the DNA would be the same weight. What appeared though was two bands. One was radioactive and the other was not. This el ...
Essential Question
Essential Question

... Essential Question What is DNA made of and how ...
The Human Genome Project
The Human Genome Project

... proteins Is there one map for every human? Now there is a database of genes. We still don’t know what all the genes do. What do genes do? Code for proteins. ...
Biotechnology - (www.ramsey.k12.nj.us).
Biotechnology - (www.ramsey.k12.nj.us).

... “Some biologists estimate that as many as 10 percent of animal species andup to 25 percent of plant species may occasionally breed with another species.” NYT ...
answers
answers

... __JAMES WATSON____ & _FRANCIS CRICK_____ used _Rosalind Franklin’s X-ray images to help them figure out the structure of DNA. SUBUNIT PROTEINS ...
TECHNICAL NOTE 4.1
TECHNICAL NOTE 4.1

... all of the “programming code” for the organism. The code for our observable characteristics (phenotype) such as hair and eye color, foot size, etc., is crammed into the nucleus.This code is called DNA (deoxyribonucleic acid). An organism’s basic complement of DNA is called its genome. DNA is essenti ...
DNA structure in the Cell
DNA structure in the Cell

... DNA – Material of Heredity Bacteria carry their genetic material as a single circular chromosome, without it being enclosed by a nuclear membrane (prokaryotes) 1923 – Frederick Griffith performed studies with Streptococcus pneumoniae Two forms - S-Strain which is wild-type and highly pathogenic - R ...
Cell wk 8
Cell wk 8

... • 2 polynucleotide chains are paired in antiparallel manner, around an imaginary common axis. ...
APBioTech 2015 16
APBioTech 2015 16

... • Gene cloning was made possible by the discovery of restriction enzymes. • Many different enzymes exist – named after the organism in which they are found • EcoRI (E. coli), HindIII (Haemophilus influenza), PstI (Providencia stuartii) ...
CBA Review
CBA Review

...  Discovery of ecosystems based on chemosynthesis in deep ...
Slideshow
Slideshow

... Note: Carriers are not always listed as they are not identified by phenotype ...
Biol 505 EXAM 1 (100 points): Due Wed 10/14/09 at the beginning
Biol 505 EXAM 1 (100 points): Due Wed 10/14/09 at the beginning

... 1. Outline the relations between genes, DNA, and chromosomes. 2. Compare and conrast genotype and phenotype. 3. What is semiconservative replication? 4. Draw a molecule of DNA undergoing eukaryotic linear replication. On your drawing,identify (1) origin, (2) polarity (5’ and 3’ ends) of all template ...
Cloning :-
Cloning :-

... DNA genome enclosed in a protein head (capsid). As with other viruses, they depend on the host cell for their propagation and do not exist as free-living organisms. Structurally, phages fall into three main groups: (1) tailless (2) Bacteriophages are essentially bacterial viruses and usually consist ...
Notes 12-1
Notes 12-1

DNA Replication
DNA Replication

... • Deletion mutation--piece of chromosome breaks off and is lost; the new DNA strands are also missing a piece • Duplication--piece of chromosome breaks off and is inserted on homologous chromosome • Translocation--piece of chromosome breaks off and attaches to a different, non-homologous chromosome ...
Chapter 3,
Chapter 3,

... Suppose you are a scientist who wants to insert into your dog a gene that encodes a protein that protects dogs from heartworms. A dog’s cells are not competent, so they cannot take up the gene from the environment; but you have a plasmid, a competent bacterium, and a related (though incompetent) F+ ...
Genes have fixed positions on chromosomes.
Genes have fixed positions on chromosomes.

... function, and produce a white kernel. When the element moves, the pigment gene function is restored, producing a reddish splotch of color on the skin of the kernel. ...
PHYS 4xx Intro 3 1 PHYS 4xx Intro 3
PHYS 4xx Intro 3 1 PHYS 4xx Intro 3

... Genetic information is stored in the DNA sequences using the GCAT alphabet. Both the sequence for a gene, and it's complement (ie, CGTA) is stored, although other information is also encoded to indicate which is the correct direction for transcription. The sequence on the DNA master blueprint corres ...
No Slide Title
No Slide Title

... transferred to yeast, other fungi, and even animal and human cells. ...
The `thread of life`, is deoxyribonucleic acid, otherwise known as
The `thread of life`, is deoxyribonucleic acid, otherwise known as

... determined gene even color involve interaction several genes some complex such cystic fibrosis wrong number places have already accounted places where cystic fibrosis mutates more being uncovered weekly many environmental factors some physical other chemical alter structure molecule mutation occurs ...
Transposable Elements
Transposable Elements

... function, and produce a white kernel. When the element moves, the pigment gene function is restored, producing a reddish splotch of color on the skin of the kernel. ...
A History of Innovation in Genetic Analysis
A History of Innovation in Genetic Analysis

... The Flavr Savr tomato, the first genetically engineered food product, is approved for market. • Applied Biosystems introduces systems that automate and standardize DNA-based technology for forensic investigation. • DNA fingerprinting using PCR becomes accepted in court as reliable forensic evidence ...
Chapter 11
Chapter 11

... C. This “transforming principle” was later identified by Avery and colleagues in 1944 as DNA D. Alfred Hershey and Martha Chase’s experiments on bacteriophages 1. Labeled the protein coat with radioactive sulfur, the DNA with radioactive phosphorus 2. Found that only the phosphorus was found in the ...
Chemical basis of Inheritance Review KEY - Pelletier Pages
Chemical basis of Inheritance Review KEY - Pelletier Pages

... 13. What role do DNA polymerase and DNA ligase play in gene replication? DNA polymerase adds DNA nucleotides to the 3’ end of the growing DNA molecule. DNA ligase forms the phosphodiester bonds between the okazaki fragments on the lagging strand. 14. What two bases can pair with adenine? T and U 15. ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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