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DNA Replication
DNA Replication

... “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material” ~Watson and Crick ...
Transcription Worksheet
Transcription Worksheet

... Write the answer to each question in the blank provided. 1. What is the enzyme that is important for the process of transcription?______________________________ 2. In DNA, what is the sugar called?___________________________________________________________ 3. What is a three nucleotide sequence of m ...
Sequencing Rationale
Sequencing Rationale

... because it is what makes up chromosomes. It relates back to how DNA is the genetic material of all living things. At this point students usually talk about DNA, but they really do not know what it is yet. In this segment, the students understand the structure of DNA, how it is replicated, and how DN ...
Transcription Worksheet
Transcription Worksheet

... Write the answer to each question in the blank provided. 1. What is the enzyme that is important for the process of transcription?______________________________ 2. In DNA, what is the sugar called?___________________________________________________________ 3. What is a three nucleotide sequence of m ...
RESTRICTION ENDONUCLEASES
RESTRICTION ENDONUCLEASES

... single stranded DNA are necessary. The identification of restriction endonucleases in the 1960s and early 1970s and the recognition that these enzymes act as “molecular scissors”, always cutting DNA at specific locations (base sequences), was the key discovery which allowed the cloning of DNA to bec ...
molecular_gene_cloning_restriction
molecular_gene_cloning_restriction

... single stranded DNA are necessary. The identification of restriction endonucleases in the 1960s and early 1970s and the recognition that these enzymes act as “molecular scissors”, always cutting DNA at specific locations (base sequences), was the key discovery which allowed the cloning of DNA to bec ...
The Universal Genetic Code - Willimon-PHS
The Universal Genetic Code - Willimon-PHS

... 2. DNA separated from cells, cut by restriction enzymes 3. Cut DNA run through gel electrophoreses, separates by size 4. Samples compared Genetic engineering – direct manipulation of genes Cloning – process used to make multiple copies of a desired gene, • Used for gene therapy, pharmaceutical drug ...
ch 3 notes
ch 3 notes

... The DNA in your bone cells is exactly like the DNA in your skin cells, which is exactly like the DNA in your hair cells, etc….. The DNA Molecule: The Genetic Code DNA: The blueprint of life Chemical template for every aspect of organisms Double helix, ladderlike structure Ladder forms nucleotide Lad ...
DNA Replication and recombination
DNA Replication and recombination

... II. DNA and RNA: Composition and Function III. History of solving the structure of DNA ...
Carcinogenesis1
Carcinogenesis1

... Malignant neoplasms are usually called carcinomas (endo- or ectoderm) or sarcomas (mesoderm). Exceptions are hematopoietic malignancies, melanoma, neuroblastoma, thymoma. ...
Problem Set 2B
Problem Set 2B

... Draw three base pairs of a DNA molecule that has the sequence 5’-GCA-3.’ Draw one strand down the page on the left, and the other strand base-paired with it just to the right. Use the same amount of detail as we used in class. Show the negative charge on each phosphate group, label the 5’ and 3’ car ...
DNA Replication - ms. velasco`s laboratory
DNA Replication - ms. velasco`s laboratory

... 2. Label one side with DNA molecule with nitrogen bases (A,T,C,G) 3. Then write the corresponding letter on the opposite side (A with T and C with G) 4. Cut between the 6th and 7th nitrogen base from the bottom and tape the top flaps together 5. On the unzipped DNA strand (the part you didn’t tape c ...
Introduction to Genetics and Genomics
Introduction to Genetics and Genomics

... Recall from "Rule of Segregation", offspring get one gene from each parent. Markers are not genes, but they are regions on chromosomes (meiosis). ...
File
File

... end of the RNA primer, Polymerase III covalently bonds the extra nucleotides creating the leading strands. ...


... 3. Change the following false statements to true statements: a. Plasmids are used as vectors in genetic engineering experiments involving humans. Rewrite: b. Recombinant DNA contains two types of bacterial DNA recombined together. Rewrite: c. Gene cloning occurs when an organism, such as a bacterium ...
Biotechnology
Biotechnology

... to the next. Not all vectors are plasmids, however. We commonly use engineered viruses, for example bacteriophage lambda, which can carry large pieces of foreign DNA. Plasmids In addition to the nucleoid, many bacteria often contain small nonchromosomal DNA molecules called plasmids. Plasmids usuall ...
How hair can reveal a history
How hair can reveal a history

... statistics. Analysts focus on 13 or more places in the genome, called loci, where humans are extraordinarily diverse. Each locus contains a “short tandem repeat,” a bit of DNA that is repeated multiple times. The exact number of repeats at each locus varies from person to person and can range anywhe ...
DNA WAS DETERMINED TO BE THE TRANSFORMING
DNA WAS DETERMINED TO BE THE TRANSFORMING

... • Swiss Physician, Johannes Friedrich Miescher isolated the chemical he called “nuclein” from the nuclei of pus cells ...
2nd Semester Biology Tournament - d
2nd Semester Biology Tournament - d

... 16. What is produced during mitosis? Include number of cells and chromosomes. 17. What is produced during meiosis? Include number of cells and chromosomes. 18. What types of cells are produced during mitosis VS meiosis? 19. What is the role of the spindle fibers in mitosis and meiosis? 20. What is a ...
CH 12: Mendel and Heredity
CH 12: Mendel and Heredity

... 1. Describe Mendel's famous pea plant experiment and tell how he created the P, F 1 and F2 generations. Show the F 1 and F2 crosses and tell what the phenotype and genotype ratios were for FI and F2. ...
DNA
DNA

... Discovery, Structure, and Replication! Part A. Go to http://www.dnaftb.org/dnaftb/15/concept/ Or Google “DNA” click go to www.dnaftb.org/dnaftb and select the “Molecules of Genetics.” Note the right side menu and that you are starting on number 15. First read the concept paragraph and answer the fol ...
013368718X_CH15_229-246.indd
013368718X_CH15_229-246.indd

... 18. An organism that contains one or more genes from another species is inbred. 19. Transgenic organisms can be made by inserting recombinant DNA into the genome of the host organism. 20. Examining the properties of a transgenic organism allows scientists to discover the function of the transferred ...
AP Biology - gwbiology
AP Biology - gwbiology

... 9. What is a complementary, short, single stranded nucleic acid that can be either DNA or RNA called? 10. Why do scientists use a radioactive isotope tag for the probes? ...
Nucleotide drug targets
Nucleotide drug targets

... The double stranded structure provides protection to the information containing face of the bases, an extra copy of the information and a template for repair. The outside of the DNA, with its predominating phosphate groups and sugar is very hydrophilic. The bases, buried in the interior, are much mo ...
DNA STRUCTURE (Sections 10.1 – 10.3)
DNA STRUCTURE (Sections 10.1 – 10.3)

... 1. First, the two strands of DNA have to separate. This occurs at an origin of replication. Bacterial chromosomes have one of these, while eukaryotic cells have numerous origins, which speeds up the process considerably. Look at Figure 10.5A to see how replication bubbles are formed and join. What i ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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