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... A. DNA and DNA replication FOCUS ON CHAPTER 16 1. DNA structure (and RNA structure) (Figures 5.26, 16.5, 16.6, 16.7, 16.8, 16.X-pg. 310) 2. DNA structure provides a mechanism for DNA replication 3. Steps in DNA replication (16.9, 16.12, 16.13, 16.14, 16.15, 16.16, 16.17) 4. DNA replication involves ...
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... a “true nucleus”. Prokaryotes (such as bacteria) do not have nuclei or ribosomes. Moreover, their DNA includes no introns. This may help them evolve faster by causing more variation among their genotypes. Eukaryotic genomes, by contrast, are more robust and stable. A particular region of DNA does no ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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