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Genetics Review
Genetics Review

mutations - Pasadena High School
mutations - Pasadena High School

... (causes the amino acid chain to stop protein production early) ...
CONFOUNDING PHYLOGENETIC TREES
CONFOUNDING PHYLOGENETIC TREES

... -according to rRNA based phylogenies, there are 3 kingdoms of life – bacteria, archaea and eukarya with eukarya derived from archaea -the sequencing of hundreds of genomes has called into question this tree because many proteins in any one organism can be archaeal or bacterial in origin -gene swappi ...
Epigenetics
Epigenetics

... around which the DNA is coiled, making gene expression easier. These additions turn the gene expression on and off, silencing some genes and activating others. They do not change the DNA but they can be inherited through epigenetic inheritance. ...
Eucharyotic Chromatin Organization
Eucharyotic Chromatin Organization

... Why is the control of gene expression more complex in eukaryotes than prokaryotes ? (2)  4) cells that require cell specialization or ...
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Nucleic Acid review

... base pairing in DNA and how many hydrogen bonds ...
Making Proteins
Making Proteins

... “stop” codon (UAA, UAG, UGA). Protein synthesis is complete. ...
Biotechnology and Genetic Engineering
Biotechnology and Genetic Engineering

... Sequences examined in DNA fingerprinting • VNTRs-variable number tandem repeats; composed of 8-80 bp repeat units (e.g., [GCGCAATG]n) which are tandemly repeated so that the overall length is 1-30 kb • STRs-short tandem repeats; composed of 2-7 bp repeat units (e.g., [AC]n) which are tandemly repea ...
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Gene Technology Quest – Study Guide KEY What is a genome? A

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3 - life.illinois.edu

... and bacterial (attB) sequences showed that the recombination occurs between attDOT and attB by staggered cleavages seven base apart on each att site. The sites of cleavage in attDOT are shown between the D and D’ sites in the sequence. In vitro experiments indicated that the IntDOT integrase, which ...
Genética Molecular em Medicina Transfusional
Genética Molecular em Medicina Transfusional

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Scientist Guide DNA Bracelet Workshop

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Tech Notes Use of Plasmid-Safe™ to Prevent Cloning Artifacts Due

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Gregor Mendel & DNA structure

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Midterm Exam Review 1. How many chromosomes are in a “normal

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Jeopardy

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Gene expression An organism`s genome is the complete set of

CHAPTER 12
CHAPTER 12

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... ______ 3. The process by which a cell makes a copy of the DNA. ______ 4. The building blocks of a protein. ______ 5. One form of a gene. ______ 6. An organism’s genetic makeup or the letters used to represent the trait. ______ 7. A chart or “family tree” that tracks the inheritance of a particular t ...
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... Decide on the ACTN3 genotype from your gel results and write it down. Note ideas on whether this type of testing is useful Note ideas on any issues it may raise FOR INTEREST: What is polymerase chain reaction (PCR)? PCR copies defined pieces of DNA sequence so there is enough to study in the laborat ...
Students Visit DNA Learning Center
Students Visit DNA Learning Center

... The size of the genome is about 165 million bases and contains and estimated 12,000 genes (by comparison, the human genome has 3,300 million bases and may have about 70,000 genes; yeast has about 5800 genes in 13.5 million base bases). ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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