Document
... •Proteins in all cells use the same 20 amino acids, synthesized in the same way,and use the same post-translation modification •Protein synthesis is carried out via translation in the same way in all organisms •Most of the reactions catalyzed by these proteins are the same and the enzymes that carry ...
... •Proteins in all cells use the same 20 amino acids, synthesized in the same way,and use the same post-translation modification •Protein synthesis is carried out via translation in the same way in all organisms •Most of the reactions catalyzed by these proteins are the same and the enzymes that carry ...
Transgenic Animal Models
... E.g., one that has the Cre recombinase in the hippocampus. There is a very large number of Cre recombinase mouse lines, each with tissue-specific, developmentally specific, or exogenous chemical specific properties (e.g., responds to tetracycline). The offspring will then have the gene knocked out i ...
... E.g., one that has the Cre recombinase in the hippocampus. There is a very large number of Cre recombinase mouse lines, each with tissue-specific, developmentally specific, or exogenous chemical specific properties (e.g., responds to tetracycline). The offspring will then have the gene knocked out i ...
Integrated Science 3/4 Course Map Biology_EOC_FAQ_2016
... 6. Why do some populations get along when others don’t? At some level, all populations compete with each other since resources (what they are after) are limited. However, it is not uncommon to see cooperation among two or more organisms if their partnership leads to benefits on both sides of the rel ...
... 6. Why do some populations get along when others don’t? At some level, all populations compete with each other since resources (what they are after) are limited. However, it is not uncommon to see cooperation among two or more organisms if their partnership leads to benefits on both sides of the rel ...
36_sequencing
... • 1990: linked the disease to a marker on Chromosome 17q21 – D17S74 - 183rd marker used! – Initial candidate region spanned half the chromosome (hundreds of possible genes . . .) ...
... • 1990: linked the disease to a marker on Chromosome 17q21 – D17S74 - 183rd marker used! – Initial candidate region spanned half the chromosome (hundreds of possible genes . . .) ...
BIOL08012 2016 May
... separation of daughter molecules, synthesis of RNA primer, unwinding of DNA double helix, attachment of single-strand binding proteins, synthesis of new DNA. unwinding of DNA double helix, separation of daughter molecules, synthesis of new DNA, attachment of single-strand binding proteins, synthesis ...
... separation of daughter molecules, synthesis of RNA primer, unwinding of DNA double helix, attachment of single-strand binding proteins, synthesis of new DNA. unwinding of DNA double helix, separation of daughter molecules, synthesis of new DNA, attachment of single-strand binding proteins, synthesis ...
Slide 1 - Loyola Blakefield
... • Enables researchers to detect whether certain DNA sequences exist in a sample. • Bands from electrophoresis are “blotted” onto a special paper, and treated with a radioactive DNA single strand. ...
... • Enables researchers to detect whether certain DNA sequences exist in a sample. • Bands from electrophoresis are “blotted” onto a special paper, and treated with a radioactive DNA single strand. ...
introductory slides
... Watson and Crick, Nature, 1953: “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material” ...
... Watson and Crick, Nature, 1953: “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material” ...
Lambda Vectors and their replication
... which can replicate their DNA independently of their bacterial chromosome. They are found naturally in bacteria and replicate inside the bacterial cell. They can insert pieces up to 10kb(kilobases) or 100 to 10,000 base pairs. Examples: pBR322 and pUC18 2. Bacteriophage l- They are double stranded l ...
... which can replicate their DNA independently of their bacterial chromosome. They are found naturally in bacteria and replicate inside the bacterial cell. They can insert pieces up to 10kb(kilobases) or 100 to 10,000 base pairs. Examples: pBR322 and pUC18 2. Bacteriophage l- They are double stranded l ...
Bio_11_Rev
... characteristics and modify plants by manipulating a plant’s genes. Genetic engineering can change plants in many ways; from making plants drought resistant to making plants that can thrive in different soils, climates or environmental conditions. Genetic engineers have developed crop plants that are ...
... characteristics and modify plants by manipulating a plant’s genes. Genetic engineering can change plants in many ways; from making plants drought resistant to making plants that can thrive in different soils, climates or environmental conditions. Genetic engineers have developed crop plants that are ...
DNA Review (study guide)
... 3. Base pairing rule states that the DNA of any species contains equal amounts of __________________ & ____________ and also equal amounts of __________________ & ____________________ 4. Wilkins and Franklin studied the structure of DNA using _________________________. 5. In DNA, thymine is compleme ...
... 3. Base pairing rule states that the DNA of any species contains equal amounts of __________________ & ____________ and also equal amounts of __________________ & ____________________ 4. Wilkins and Franklin studied the structure of DNA using _________________________. 5. In DNA, thymine is compleme ...
2011 Spring Biology Final Review
... 2. Read the above paragraph. Using your own words, evaluate this statement. Support it as either a valid or an invalid argument for evidence of evolution occurring on this planet. ...
... 2. Read the above paragraph. Using your own words, evaluate this statement. Support it as either a valid or an invalid argument for evidence of evolution occurring on this planet. ...
SBI4U: Molecular Genetics Unit Review
... 17. Describe what happens in initiation, elongation, and termination of: ...
... 17. Describe what happens in initiation, elongation, and termination of: ...
Viral particles
... • Typically, genomic material of phage is 25-50% of virus mass • Usually the viral genomes tightly packed with “useful" info (e.g., 90% of M13 genome represents protein coding region) • Phage particles are metabolically inactive, but they may contain enzymes involved in virus proliferation (e.g., ly ...
... • Typically, genomic material of phage is 25-50% of virus mass • Usually the viral genomes tightly packed with “useful" info (e.g., 90% of M13 genome represents protein coding region) • Phage particles are metabolically inactive, but they may contain enzymes involved in virus proliferation (e.g., ly ...
Document
... • Warm (72°C) to activate Taq polymerase, which extends primers and replicates DNA • Repeat multiple cycles ...
... • Warm (72°C) to activate Taq polymerase, which extends primers and replicates DNA • Repeat multiple cycles ...
Nucleic acid recognition from prokaryotes to eukaryotes: Case
... small angle X-ray scattering data, we propose a model of adjustable binding registers as a means for universal recognition of diverse 3' splice sites by U2AF65. Altogether, these two examples demonstrate the diversity of conformational changes used by proteins to accomplish gene regulation, from the ...
... small angle X-ray scattering data, we propose a model of adjustable binding registers as a means for universal recognition of diverse 3' splice sites by U2AF65. Altogether, these two examples demonstrate the diversity of conformational changes used by proteins to accomplish gene regulation, from the ...
Worksheet for Biology 1107 Biological Molecules: Structure and
... Using the text provided on the web site and using the models of the molecules provided in lab: answer the following questions, and turn them in to your instructor. 1. The two groups of monosaccharides that are most important to biologists have how ...
... Using the text provided on the web site and using the models of the molecules provided in lab: answer the following questions, and turn them in to your instructor. 1. The two groups of monosaccharides that are most important to biologists have how ...
Bacterial Genetics
... - phage injects its DNA into bacteria (host #1) - Host DNA is fragmented - Phage uses host DNA to make its own proteins and replicate its DNA - Host DNA is packaged into new viral capsid (not on purpose) - Lysis of host cell releasing many phages with host’s (#1) DNA - Phage with host’s #1 DNA infec ...
... - phage injects its DNA into bacteria (host #1) - Host DNA is fragmented - Phage uses host DNA to make its own proteins and replicate its DNA - Host DNA is packaged into new viral capsid (not on purpose) - Lysis of host cell releasing many phages with host’s (#1) DNA - Phage with host’s #1 DNA infec ...
Applying Bayes` Theorem to DNA Sequence for Identification of
... To develop an easy, simple method for identifying microorganisms based on their DNA sequences, Bayes' theorem was applied to DNA sequence analysis. It was hypothesized that the conditional probability of a DNA sequence from an unknown bacterial species being a member of a particular species could be ...
... To develop an easy, simple method for identifying microorganisms based on their DNA sequences, Bayes' theorem was applied to DNA sequence analysis. It was hypothesized that the conditional probability of a DNA sequence from an unknown bacterial species being a member of a particular species could be ...
Unit 4 Review 1. When are gametes produced? 2. What results at
... Draw and label a Punnett square. Be able to identify parent genotypes, offspring genotypes, ratios, list ALL details. ...
... Draw and label a Punnett square. Be able to identify parent genotypes, offspring genotypes, ratios, list ALL details. ...
As a group, quietly discuss each question and agree
... 6 of 15) Three enzymes have been discussed thus far: DNA Helicase, RNA polymerase, and DNA polymerase. Part A: During DNA replication, which enzyme breaks the hydrogen bonds allowing the DNA to separate? ...
... 6 of 15) Three enzymes have been discussed thus far: DNA Helicase, RNA polymerase, and DNA polymerase. Part A: During DNA replication, which enzyme breaks the hydrogen bonds allowing the DNA to separate? ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.