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Plant Transformation
Plant Transformation

from innovative technologies ...to superior key products
from innovative technologies ...to superior key products

... Nucleic acids store and transfer genetic information in cells. The main types of nucleic acids are DNA and R NA, which are made up of chains of chemicals called nucleotides. Most DNA exists in cells as a double-stranded structure that resembles a twisted ladder. The nucleotides on opposite sides of ...
Forside eksamen bokmål NTNU
Forside eksamen bokmål NTNU

... Regulation of gene expression in eukaryotes is controlled by a number of DNA binding proteins. We often want to identify these proteins and determine where in the genome and which DNA sites they bind. a. Describe two methods that can be used to isolate DNA binding proteins. b. You have isolated a tr ...
Models for homologous recombination
Models for homologous recombination

... Programmed generation of double –stranded DNA breaks occurs during meiosis MRX protein processes the cleaved DNA ends for assembly of the RecA-like strand –exchange proteins Dmc1 is a RecA-like protein that specifically functions in meiotic recombination Many protein function together to promote mei ...
AP Biology Ch. 12 Reading Guide – Molecular Biology of the Gene
AP Biology Ch. 12 Reading Guide – Molecular Biology of the Gene

... 10. What technique did Rosalind Franklin do to help Watson and Crick in determining a model for DNA? 11. Briefly describe Watson and Cricks Model. ...
13. DNA Replication
13. DNA Replication

... 1. Review of DNA structure DNA double helix model:  DNA made of nucleotide building blocks linked into polymer chains  Bases are on inside, sugars and phosphates form a backbone on outside  Two strands exist in an antiparallel arrangement ...
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What would we like to know about DNA and how do we obtain that

... • Sequencing an organisms entire genome • Why would we want to do this? ...
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11th Grade Science PPT

... A. DNA is composed of only 4 types of nucleotides. B. DNA is composed of smaller subunits than are proteins. C. DNA is abundant in both the nucleus and the cytoplasm. D. The concentration of DNA is generally consistent from cell to cell. ...
Mutation - WordPress.com
Mutation - WordPress.com

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DNA replication

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What does DNA look like?

Name____________________________ DNA Investigation
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ChapteR 16 The molecular basis of inheritance

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Classification of Microorganisms

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... Registry, December 2010). Benzene is a DNA-reactive carcinogen that binds to the DNA of a cell and damages its structure resulting in adduct formation (insertion of the benzene metabolite into the DNA) and chromosome breakage. These damaged portions of the DNA may remain as lesions in the parental D ...
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AP Biology Unit 4 Continued

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DNA WebQuest

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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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