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... quickly. For example, the number of DNA bases in the genome of a human is approximately 3 billion. The sequencer can determine the sequence of this huge number of DNA bases in one day, which is a process that took years to complete when the human genome was first sequenced. “I am very excited about ...
A1988L264200002
A1988L264200002

... methylase that would postreplicationally form 5’-methylcytosine at symmetrical DNA sites. It was further proposed that thisenzyme would prefer hemimethylated sites. With such an enzyme, methylation patterns would be somatically heritable and could be important for X-chromosome inactivation and cellu ...
Level 3 Biology (90715) 2011 Assessment Schedule
Level 3 Biology (90715) 2011 Assessment Schedule

... Crossing over separates P and L (purple and long) and p and l (red and round) in a few cases. This makes the other 2 phenotypes possible. ...
MITOSIS -Cells undergo cell division
MITOSIS -Cells undergo cell division

... -Cells undergo cell division- to replace cells that have been lost due to maturation/apoptosis -cells also divide- inresponse to an increased work load -seen by light microscope- labelled radioactive DNA precursors such as H3 Thymidine- recorded using autoradiographic methods G0 phase -most of the c ...
Assessment Schedule
Assessment Schedule

... Crossing over separates P and L (purple and long) and p and l (red and round) in a few cases. This makes the other 2 phenotypes possible. ...
Genetic engineering and biotechnology techniques
Genetic engineering and biotechnology techniques

... genome mapping before cheaper methods such as PCR (polymerase chain reaction) and DNA sequencing came along ...
BACKGROUND INFORMATION:
BACKGROUND INFORMATION:

... Recombinant DNA refers to DNA of one organism inserted into the DNA of another. The major tools of recombinant DNA technology are bacterial enzymes called restriction enzymes. Each enzyme recognizes a short, specific nucleotide sequence in DNA molecules, and cuts the backbones of the molecules at th ...
B8. Nucleic Acids (HL)
B8. Nucleic Acids (HL)

... each codon corresponds to a specific amino acid – This is known as the genetic code and it is both universal and degenerate • These amino acids will be brought to the ribosome by tRNA and the formation of a polypeptide will commence • Once the specific protein is formed, the mRNA, rRNA, and tRNA wil ...
BIOTECHNOLOGY AND GENETIC ENGINEERING
BIOTECHNOLOGY AND GENETIC ENGINEERING

...  -(HGP)sequence all the base pairs in the human genome (2-3 billion pairs) ...
the language of biology - Gonzaga College High School
the language of biology - Gonzaga College High School

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What has changed - Center for Genetics and Society

... Unpredictabilities and Risks Off-target effects (mutations in the genome) a) changes of function of proteins (if in coding sequence) b) changes in the expression of genes, eg resulting in: increased presence of plant toxins, absence of proteins important for nutrition, plant defence or disease resi ...
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20 - Biotechnology

... “Pharm” animal: produce human protein secreted in milk for medical use ...
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Supplemental File S10. Homologous

... Deoxyribonucleic acid (DNA): The genetic material of most organisms. DNA is a long doublehelical molecule composed of the deoxyribonucleotides-deoxyadenylic acid (A), dexythymidylic acid (T), deoxyguanylic acid (G), and deoxycytidylic acid (G). The two strands are held together by hydrogen bonds bet ...
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Biotechnology

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DNA to Proteins to Natural Selection - Cal State LA
DNA to Proteins to Natural Selection - Cal State LA

... alters small segments of DNA, usually within a single gene b. Beneficial = increases the survival or ability of an individual to reproduce; rare; alters small segments of DNA, usually within a single gene c. Lethal = eventually leads to an individual’s death or inability to reproduce; common; alters ...
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Lecture 2: Biology Review II

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pGLO TM Bacterial Transformation

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... 4. Watson and Crick were the first to suggest that DNA is _____. a. a short molecule c. a protein molecule b. the shape of a double helix d. the genetic material 5. The chromosome abnormality that occurs when part of one chromosome breaks off and is added to a different chromosome is _____. a. delet ...
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... ‘made it possible to go back in time and study old DNA sequences. Indeed, the PCR can even recreate longer sequences from short fragments in the test tube [2]. Thus, we can now dream :about catching molecular evolution red-handed. So far, dlreams about this sort of time travel have come true only fo ...
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Francis Crick - WordPress.com

... Using X-ray diffraction studies of DNA, in 1953, James Watson and Crick constructed a molecular model representing the known physical and chemical properties of DNA. It consisted of two intertwined spiral strands, resembling a twisted ladder (referred to as the "double helix"). They hypothesized tha ...
Viruses, Jumping Genes and Other Unusual Genes
Viruses, Jumping Genes and Other Unusual Genes

11-7-12 Cellular Reproduction PPT FILL IN THE BLANK NOTES
11-7-12 Cellular Reproduction PPT FILL IN THE BLANK NOTES

... 300 pairs human 23 pairs Chromosomes __________________ in sexually reproducing organisms. One of the pair comes from the ________________, the other comes from the ______________. ________________________________________: two members of a pair of chromosomes that carry genes for the same traits, ha ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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