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1 The structure and replication of DNA
1 The structure and replication of DNA

... base. These nucleotides join together to form a sugar–phosphate backbone. - Base pairing occurs between the two strands of DNA between adenine, thymine and guanine, cytosine. These base pairs bond by weak hydrogen bonds. - The DNA helix is double stranded, and has an anti-parallel structure, with de ...
Course Outline - Pima Community College
Course Outline - Pima Community College

... PIMA COMMUNITY COLLEGE Effective Term: Fall 2009 ...
Cancer results from an accumulation of mutations which
Cancer results from an accumulation of mutations which

BXCC overview - Harlem Children Society
BXCC overview - Harlem Children Society

... got a sheet with base sequences of DNA. Then we compared them with our partners. We had to see how the base sequences are similar, different and if we think both DNA’s will have the same proteins. There was then another paragraph that stated that genes aren’t able to leave the nucleus to carry the i ...
DNA
DNA

... nucleoside triphosphate. • This molecule is similar to ATP except the sugar is deoxyribose and the sugar in ATP is ribose. • The energy comes from the hydrolysis of the phosphate tail. ...
Title: Ready, Set, Clone! Authors: Kowalski, Kathiann M. Source
Title: Ready, Set, Clone! Authors: Kowalski, Kathiann M. Source

... that we're interested in," says biologist Clare O'Connor at Boston College. Probably the biggest DNA cloning job so far was the Human Genome Project, which figured out the order of the 3 billion base pairs in human DNA. That 13-year job involved making many copies of DNA pieces that were up to 1,000 ...
Review Sheet—Cell Division
Review Sheet—Cell Division

... 29. Mutations can be passed on to offspring if they occur during which process: Mitosis or Meiosis? Meiosis 30. Mutations in which of these cells are not passed on to offspring: hair cell, sperm cell, heart cell, and blood cell. 31. A __________ in an organism’s DNA causes a change in the production ...
Southern transfer
Southern transfer

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Molecular biology: Gene cloning
Molecular biology: Gene cloning

... copies not only of itself, but also of the gene that it carries. When the host cell divides, copies of the recombinant DNA molecule are passed to the progeny and further vector replication takes place. After a large number of cell divisions, a colony or clone of identical host cells is produced. Eac ...
DNA (Deoxyribonucleic acid)
DNA (Deoxyribonucleic acid)

... If one base was equal to one amino acid, the maximum codes would be 4. If two bases was equal one amino acid, the maximum would be 16 codes. If three bases equal one amino acid, the maximum codes would be 64. ...
GENeS “R” US - Nanyang Technological University
GENeS “R” US - Nanyang Technological University

... The study of genes and DNA is fascinating. Since the days of Gregor Mendel, an Austrian monk who puttered in a monastery garden, to Watson and Crick, who figured out the structure of the DNA molecule, to Ian Wilmut, who cloned Dolly the sheep from an adult ewe’s DNA, a tremendous number of things ha ...
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BACKGROUND: UvrC is a DNA repair enzyme found in all

幻灯片 1
幻灯片 1

... 1. Recombination is a one-way process in procaryotes: a piece of genetic material (the exogenote) is donated to the chromosome of a recipient cell (the endogenote) and integrated into it. 2. The actual transfer of genetic material between bacteria usually takes place in one of three ways: direct tra ...
DNA, RNA, and PROTEIN SYNTHESIS SUMMERY QUESTIONS
DNA, RNA, and PROTEIN SYNTHESIS SUMMERY QUESTIONS

... Translation: The Decoding of mRNA Codon/Anticodon: Alignment keeps the proper order of Amino Acids Order: The Order of Amino Acids is important for the SHAPE and FUNCTION of the Protein 4) There can be 10 million to 20 million proteins in the average Human (Eukaryotic) cell. a) Briefly explain HOW t ...
Concept Check Questions with answers
Concept Check Questions with answers

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Systematic Implications of DNA variation in subfamily

... Should be present in all taxa to be compared Must have some knowledge of the gene or other genomic region to develop primers, etc. Evolutionary rate of sequence changes must be appropriate to the taxonomic level(s) being investigated; “slow” genes versus “fast” genes It is desirable that sequences c ...
DNA Recombinations
DNA Recombinations

... One method for doing this is to use plasmid DNA from bacteria. Small DNA fragments can be inserted into the plasmids, which are then introduced into bacterial cells. As the ...
Unit 4 Objectives
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Chapter 12 DNA and RNA - Northwestern High School
Chapter 12 DNA and RNA - Northwestern High School

... • Messenger RNA (mRNA) – carry copies of instruction from DNA • Ribosomal RNA (rRNA) – part of ribosome’s where proteins are assembled. • Transfer RNA (tRNA) – Transfers each amino acid to ribosome from codes. ...
N6-(6-Aminohexyl)dATP - Thermo Fisher Scientific
N6-(6-Aminohexyl)dATP - Thermo Fisher Scientific

... that contains a primary amino group attached via a 6-carbon linker at the N6position of the purine base1. AHdATP can be incorporated into DNA by nick translation in the presence of dTTP, dGTP, and dCTP. Life Technologies recommends incorporating AHdATP into DNA probes using the Nick Translation Syst ...
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Brooker Chapter 10

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File - Siegel Science

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Human Genome Project

... mediocre at best. Humans, especially those who are experts in the field, do a much better job of evaluating evidence and deciding what a given gene’s function is. There is a big problem of too much information not uniformly coded or maintained. The scientific literature contains numerous examples of ...
Chapter 10 The Code of Life Test Review Name
Chapter 10 The Code of Life Test Review Name

... 22. DNA replication ensures that each daughter cell has an exact copy of the DNA from the parent cell. 23. DNA molecules are in the shape of a double helix. 24. Genes control the production of proteins. 25. The code for one amino acid is made of 3 bases. 26. A parent organism has the base sequence A ...
from innovative technologies ...to superior key products
from innovative technologies ...to superior key products

... Nucleic acids store and transfer genetic information in cells. The main types of nucleic acids are DNA and R NA, which are made up of chains of chemicals called nucleotides. Most DNA exists in cells as a double-stranded structure that resembles a twisted ladder. The nucleotides on opposite sides of ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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