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Download 2. ANIMAL CELLS AND TISSUES Objectives After completing this
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2. ANIMAL CELLS AND TISSUES Objectives After completing this exercise you should be able to 1. Understand the cell theory and the basic structure of an animal cell. 2. Differentiate between prokaryotic and eukaryotic cells. 3. Relate the concepts of cells, tissues, organs, organ systems and organisms. 4. List the four basic types of tissues with examples. 5. Identify and describe the four tissues types and their subtypes. Introduction Animals are composed of cells and are organized in hierarchical levels. The study of the complex form and function of animal body begins with the understanding of cells, tissues and organs. The structure called as “cell”, was so named and first observed by Robert Hooke through his modified microscope. The cell is the structural and fundamental unit of life. The cell theory was developed much later in nineteenth century by a botanist Matthias Schleiden, and a zoologist Theodor Schwann. A group of similar cells carrying out common functions make a tissue. Different tissues combine together make an organ, while various organs make a system; systems, in turn, form an organism. Cell Theory The cell theory is summarized as follows: 1) All organisms are composed of cells. 2) All cells derive from previously existing cells. 3) Cells carry out all the vital functions of the organism. 4) Cells contain hereditary material that regulates cell functions and which is passed on to the next generation of cell. Cell Structure A typical animal cell has a cell membrane that encloses the cytoplasm and a well defined nucleus. The nucleus houses DNA, the hereditary material. The cytoplasm contains of membrane bound organelles like endoplasmic reticulum, Golgi apparatus, mitochondria, vacuoles, ribosome’s and lysosomes. The size of a cell is determined by the ratio of its cell surface to its volume that decides its transporting efficiency. Cell Types 1) Prokaryotic cells . 2) Eukaryotic cells Plant cells Animal cells TYPES OF CELLS Prokaryotic cells Eukaryotic cells • Means “before nucleus” • Means “true nucleus” • Do not have a nucleus • Have a membrane-bound nucleus • Without membrane- bound organelles • Have membrane- bound organelles • Do not have cytoskeleton of microfilaments and microtubules • Cytoskeleton made of microfilaments and microtubules • Are very small in size • Are comparatively bigger in size • Eg. Domain Eubacteria and Archaea • Eg. Domain Eukarya – Animal cells Animal Cell Structure A typical animal cell has a cell membrane, hereditary material in the nucleus; cytoplasm along with organelles and membrane bound structures. Figure 1: Animal Cell EXERCISE Animal cells: I] IDENTIFICATION OF CELL PARTS 1. Using figure 1 and the models study the organelles of an animal cell. 2. Make a list of structures you identify in the model. II] PREPARATION OF STAINED WET MOUNT 1. Take a slide with a small drop of methylene blue. 2. Scrape the inner lining of your cheek using a toothpick and roll it in the drop of methylene blue. 3. Place a cover slip over it, and observe under scanning, low power and high power lenses of compound microscope; sketch below. Scanning Lens Low Power Lens High Power Lens Animal tissues: Tissue is a group of similar cells specialized for a common function. The four basic types of tissues are A) Epithelial B) Connective C) Muscle D) Nervous A) Epithelial Tissue: A sheet of cells that line the surface of the structures in the body. Functions: 1)Absorption – intestine 2)Transportation – kidney tubules 3)Excretion – sweat glands 4)Protection – skin 5)Sensory Reception – tongue The classification of Epithelial Tissue is made on the basis of1) Number of layers 2) Shape of cells 1) Number of layers – i) Simple – A single layer of cells. ii) Stratified – Two or more layers of cells. I iii) Pseudostratified – Single layered, but appears to be two layered i) Simple – Single layer of cells, eg. Blood Vessels Observe Sketch ii) Stratified – Two or more layers of cells, eg. Lining of the oesophagus Observe Sketch iii) Pseudostratified – Single layered, but appears to be two layered, eg. Tracheal lining Observe Sketch 2) Shape of cells – i) Squamous – Thin flattened cells. ii) Cuboidal – Cube like cells . I iii) Columnar – Elongated and column shaped i) Squamous – Thin flattened cells, eg. Vagina Observe Sketch ii) Cuboidal – Cube like cells, eg. Thyroid gland Observe Sketch iii) Columnar – Elongated cells, eg. Stomach Observe Sketch B) Connective Tissue: Is found throughout the body and has three main components cells, fibers and extracellular matrix. Functions: 1)Support – loose , fibrous, bone and tendon 2)Shock absorber – cartilage , adipose 3)Storage and Protection – adipose 4)Transportation – blood Types of connective tissue 1) Loose connective tissue 2) Fibrous connective tissue 3) Bone 4) Cartilage 5) Adipose 6) Blood 1) Loose connective tissue, eg. Areolar tissue Observe Sketch 2) Fibrous connective tissue, eg. Tendon Observe Sketch 3) Bone Observe Sketch 4) Cartilage Observe Sketch 5) Adipose: Observe Sketch 6) Blood Observe Sketch C) Muscle Tissue: Muscle cells contain contractile filaments that move past each other and change the size of the cell. Functions: 1)Movement - Cells contract and relax and bring about movement of the structures to which they are attached. Types: They are of three main types 1) Smooth 2) Skeletal 3) Cardiac 1) Smooth, eg. stomach Observe Sketch 2) Skeletal, eg. attached to bones Observe Sketch 3) Cardiac, eg. heart wall Observe Sketch D) Nervous Tissue: Regulates and controls body functions. It is found in brain, spinal cord and nerves. Types and Functions: Nervous tissue is composed of two types of cells. 1) Neurons - Conduction of impulse 2) Neuroglia cells - Nourishment and protection Observe Neurogliacells Sketch Neurons