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Name _____________________________________ Period _______ Changing of Gene Frequencies in Beetles: Does a Change in Gene Frequency Cause Evolution? (15 points) Beetle Population Excel Activity Part 1. Calculating Gene Frequency in a Population 1. Get a bag of “beetles” from Ms. Lykens 2. Open the “Gene Frequency Simulation” file under class files 3. Count how many homozygous dominant, heterozygous, and homozygous recessive organisms you have and record in # of Beetles for Year 1 4. Record the total beetles under Total Hint: You can do this by counting all beetles or adding together the # of beetles 5. Calculate the gene frequency of the organism by one of the following methods: a. Use a calculator to divide the # of Beetles by Total for each phenotype b. Using excel language to calculate gene frequency by inputting =(# of Beetles/ Total) in gene frequency box Which genotype has the highest frequency in the population in year 1? What is this phenotype? _______________________________________________________________________________________ Theoretically, what type of environment do you believe these beetles are living in based on the beetle that has the highest fitness? (e.g brown soil, black volcanic rock, sand, etc.) _______________________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ What is the process called in which the phenotypes in a population are “selected” for based on the surrounding environment? Hint: Think of last unit _______________________________________________________________________________________ Would the beetle population be able to evolve without mutations within the population? Explain using the words allele, phenotype, and environment. _______________________________________________________________________________________ _______________________________________________________________________________________ Part 2. Changing Gene Frequency with Changing Environment The environment in which the beetles live has changed from a rocky landscape to a field full of wild flowers. Simulate how the gene frequencies have changed over the next 1,500 years due to this selective pressure. 1. The driving force of evolution is DNA mutation—pick a handful of a “mutated” beetles from Ms. Lykens’ desk 2. Record the new phenotype (red, yellow, or green) and the new genotype (Bb) in your excel file below aa White in Year 1 3. Count the number of colored beetles and record here: __________ 4. Follow the rules specific to the color of mutant beetle you received: Red Beetles: Red Beetles Black Beetles Brown Beetles White Beetles Yellow Beetles: Yellow Beetles Black Beetles Brown Beetles White Beetles Year 500 Grew by 560 (add to # you wrote in 3) Grew by 75 Declined by 6 Declined by 10 Year 1,000 Grew by 555 Year 1,500 Grew by 600 Grew by 40 Declined by 9 Declined by 12 Grew by 34 Declined by 12 Declined by 16 Year 500 Grew by 545 (add to # you wrote in 3) Grew by 30 Declined by 4 Declined by 15 Year 1,000 Grew by 590 Year 1,500 Grew by 612 Grew by 20 Declined by 10 Declined by 20 Declined by 2 Declined by 11 Declined by 30 Year 500 Grew by 512 (add to # you wrote in 3) Grew by 66 Declined by 7 Declined by 11 Year 1,000 Grew by 553 Year 1,500 Grew by 630 Grew by 2 Declined by 11 Declined by 12 Declined by 20 Declined by 20 Declined by 20 Green Beetles: Green Beetles Black Beetles Brown Beetles White Beetles 5. Record the total for each box under Total Hint: You can do so using a calculator or excel language by inputting =(SUM(click boxes)) 6. Calculate the gene frequency of the organism for each year by one of the following methods: a. Use a calculator to divide the # of Beetles by Total for each phenotype b. Using excel language to calculate gene frequency =(# of Beetles/ Total) Which genotype/phenotype is the most common in the population after 1,500 years? ___________________ What genotypes/phenotypes are still present in the population? ______________ Explain why at 1,500 years the mutant beetle has the highest gene frequency in the population. _______________________________________________________________________________________ _______________________________________________________________________________________ What happened to the original beetle genes in the population over 1,500 years? _______________________________________________________________________________________ _______________________________________________________________________________________ How did the genotypic makeup of this population change over the past 1,500 years? Use the words allele and phenotype in your explanation. _______________________________________________________________________________________ _______________________________________________________________________________________ In nature, what do you think will happened to the black, brown, and white beetles as the red/green/yellow continue to survive and reproduce? _______________________________________________________________________________________ _______________________________________________________________________________________ Check Point: Get Ms. Lykens’ signature ______________ Part 3. Graphing Gene Frequency Once you complete parts 1 and 2, you should see a graph on your excel spread sheet. This graph is not complete. 1. Add an x and y axis title to the graph by a. Click on graph b. Click the plus sign in the top right hand corner c. Check Axis Titles d. Determine which titles to put in the axis 2. Add a title to the graph a. Click on graph b. Click the plus sign in the top right hand corner c. Check Chart Title d. Determine which titles to on your graph using the following outline: Chart Title Your Name, Period ___ 3. Print your graph to printer 11 (see board for instructions)