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Download Pharos university Faculty of Allied Medical SCIENCE Biochemistry 1
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Dr. Tarek El Sewedy Department of Medical Laboratory Technology Faculty of Allied Medical Sciences Amino acids and proteins Part 2 INTENDED LEARNING OUTCOMES By the end of this lecture, students will learn: 1. Amino Acids classification. LECTURE CONTENT • Classification of amino acids • Amino Acids and proteins nutritional facts • The basic structure of an amino-acid molecule consists of a carbon atom bonded to an amino group (-NH2), a carboxyl group (-COOH), a hydrogen atom, and a fourth group that differs from one amino acid to another and often is referred to as the-R group or the side chain. • The-R group, which can vary widely, is responsible for the differences in chemical properties. α, β, or γ Amino Acids Amino acids may be characterized as α, β , or γ depending on the location of the amino group on the carbon chain. α are on the carbon adjacent to the carboxyl group. β are on the 2nd carbon γ on the 3rd carbon from the carboxyl group. α− amino acids Amino acids found in proteins are α− amino acids. The amino group is always found on the carbon adjacent to the carboxyl group Metabolic Classification of the Amino Acids Essential and Non-essential Glucogenic and Ketogenic Essential Amino Acids Only 11 Of the 20 amino acids that make up proteins can be synthesized by the human body The other 9 amino acids must be acquired from food sources. These amino acids are known as essential amino acids Non essential Amino Acids Glucogenic or ketogenic Amino acids Glucogenic amino acids are degraded to compounds that can be used as carbon skeletons for glucose synthesis via gluconeogenesis. Ketogenic amino acids are degraded to compounds that can only be used to generate the ketone bodies. Glucogenic Amino Acids Ketogenic Amino Acids Metabolized to acetyl CoA or acetoacetyl CoA then to ketone bodies Leucine Lysine Amino Acid Functions 1. Amino acids are the building blocks of proteins. 2. Some amino acids and their derivatives function as neurotransmitters hormones. 3. It may supply the carbohydrates pool with glucose ?????????. Protein functions 1. - Catalytic functions [enzymes] 2. - Receptor [insulin receptor] 3. - Structural function [collagen] 4. - Transport [hemoglobin] 5. - Protective functions [Antibodies] Protein function (cont…) 6. - Hemostasis [clotting factors] 7. - Hormonal functions [insulin, glucagon, GH] 8. - Control of gene expression [transcription factors] 9. - DNA packing [histones] 10. - Act as buffers. Protein nutritional facts Spirulina quinoa soybeans Almond Good sources of protein Amino Acid nutrition Our body doesn’t use proteins in a direct way; first proteins are decomposed into amino acids and then amino acids are used for the resynthesize of muscular proteins Protein and diet Protein and diet • An individual's daily protein requirement depends on several factors, including: 1. Age - a growing child's needs will not be the same as an individual aged 80 years 2. Sex - males generally require more protein than (non-pregnant or non-breastfeeding) females 3. Weight - an individual who weighs 100 Kg will require more protein compared to somebody who weighs 50 Kg. 4. Muscular exertion - an individual who earns his living delivering pianos will require more protein than a computer programmer of the same age and height. 5. Muscle mass - a muscle-bound weight trainer will need more dietary protein than a marathon runner 6. Health - a person who is recovering after an illness or medical procedure may need more dietary protein than other people. • Percentage of energy that should come from protein: • Children aged 1 to 3 years - 5% to 20% • Children aged 4 to 18 years - 10% to 30% • Adults - 10% to 35% • According to the US Department of Agriculture, our protein intake should be: • Infants - 10 grams per day • Teenage boys - up to 52 grams per day • Teenage girls - up to 46 grams per day • Adult men- approximately 56 grams per day • Adult women - approximately 46 grams per day • Pregnant or lactating (breastfeeding) women - about 71 grams per day Protein deficiency Mental retardation and reduced IQ. Growth problems Wasting and shrinkage of muscle tissue Apathy Swollen belly Anemia Weaker immune system Protein Excess Weight gain. Intestinal irritation. Risk of heart disease Kidney problems Gout disease أالء صالح الخضري ASSIGNMENTS • Selected students are requested to prepare slides about one of the following topics (To be delivered before next lecture): • Digestion of proteins • Essential amino acids. • Non essential amino acids • Physical properties of amino acids. • Chemical properties of amino acids. • Disease resulting from disturbance in amino acid metabolism. • Ketone bodies and amino acids • Translation of RNA Study Question Write three points about : Classification of amino acids 1 2 3- Suggested readings: Principles of Biochemistry, Donald J. Voet, Judith G. Voet, Charlotte W. pratt; Willey, 3rd ed.