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1.
a.
b.
c.
d.
e.
2.
a.
b.
c.
d.
e.
A __________ is a small, circular DNA molecule that is separate from the
much larger bacterial chromosome.
Plasmid
Restriction enzyme
Genetic marker
DNA fingerprint
Stem cell
Placing a recombinant plasmid into a bacterial cell to make many copies of
a desired gene is called:
Nucleic acid probing
Antibiotic resistance
Gene cloning
DNA fingerprinting
Cellular differentiation.
3. Which choices are NOT involved with making recombinant DNA? (More than 1)
a.
Isolating genes with restriction enzymes.
b.
Separating DNA fragments with gel electrophoresis.
c.
Having ligase glue sticky ends of DNA together.
d.
Bacteria reproducing copies of DNA
e.
Manipulating embryonic stem cells to make specialized tissues.
4. The role of restriction enzymes is to:
a.
Carry genetic information
b.
Paste DNA fragments together.
c.
Cut DNA into fragments
d.
Repair the DNA backbone
e.
Spread antibiotic resistance.
5. The complete collection of cloned DNA fragments from one organism Is contained
in a(n):
a.
Restriction enzyme
b.
Genomic library
c.
Nucleic acid probe
d.
Genetic marker
e.
Polymerase chain reaction
6. Combining genes from different sources is known as:
a.
DNA fingerprinting
b.
Creating a genomic library
c.
Gene cloning
d.
Gel electrophoresis
e.
Recombinant DNA technology
7. Scientists often genetically modify a plant by:
a.
Infecting a cultured plant cell with a soil bacterium that contains a plasmid with
a desired gene.
b.
Injecting the desired gene into a fertilized egg
c.
Soaking a plant seed in a solution of DNA with the desired gene.
d.
Grafting a section of one plant to another.
8. How is an entire animal cloned?
a.
By inserting the nucleus of a fertilized egg into a bacterial cell.
b.
By fertilizing an egg with sperm from a different species.
c.
By injecting the desired set of genes into the fertilized egg.
d.
By injecting the desired set of genes into the unfertilized egg.
e.
By replacing the nucleus of the unfertilized egg with the nucleus from an adult
animal.
9. Which organisms are most commonly used to create recombinant DNA? (circle all
that apply).
a.
Single-celled protists
b.
Bacteria
c.
Viruses
d.
Plants
10. The polymerase chain reaction is a technique that …
a.
Makes copies of a specific segment of DNA without using living cells.
b.
Makes copies of a specific segment of DNA using living cells.
c.
Makes copies of fertilized egg cells.
d.
Makes copies of stem cells.
e.
Makes copies of restriction enzymes.
11.
a.
b.
c.
d.
e.
A technique that uses an electric current to separate DNA fragments is called:
PCR
Cellular differentiation
Gel electrophoresis
A nucleic acid probe
Recombinant DNA technology
12. Gel electrophoresis sorts DNA fragments of different
a.
charges
b.
lengths
c.
sequences
d.
Temperatures
e.
colors
13. Particular stretches of DNA that vary between individuals are called:
a.
Gene expression
b.
Restriction enzymes
c.
Genomic libraries
d.
Stem cells
e.
Genetic markers
14. A small sample of DNA can be obtained from a crime scene. Which process is
used to make multiple copies of the DNA for analysis?
a.
Cellular differentiation
b.
PCR
d.
Recombinant DNA technology
e.
Animal cloning
15. Transcription is turned off in a prokaryote by a molecule called a(n):
a.
operon
b.
promoter
c.
operator
d.
Repressor
e.
Transcription factor
16. Transcription in eukaryotes is regulated by a class of proteins called?
a.
operons
b.
promoters
d.
operators
e.
Transcription factors
17. The transcription and translation of genes into proteins is called:
a.
Cellular differentiation
b.
Gene expression
c.
PCR
d.
A Genomic library
e.
Gel electrophorsesis
18. Stem cells are so valuable to genetic therapies because…
a.
They contain better quality DNA than specialized cells.
b.
They have the ability to become any type of specialized cell.
d.
They are the main ingredient in life-saving vaccines.
e.
All of the above.
19. Which choices are sources of stem cells for research? (Circle all that apply)
a.
Adult Red blood cells
b.
Umbilical cord blood
c.
Bone marrow
d.
Embryoes
20. Unlike therapeutic cloning, reproductive cloning is …
a.
legal
b.
Implants embryonic cells into surrogates to achieve pregnancy.
d.
Creates specialized tissues from stem cells.
e.
All of the above.
21. When making recombinant DNA, scientists should
a.
Cut plasmids and desired genes with as many restriction enzymes as possible.
b.
Cut plasmids and desired genes with the same restriction enzyme.
c.
Only cut the plasmid with a restriction enzyme.
d.
Only cut the desired gene with a restriction enzyme.
22. Your DNA fingerprint refers to …
a.
Your blood type
b.
The unique ink print produced by your fingertips
d.
The pattern of DNA fragments of different lengths when run through gel
electrophoresis.
e.
The sequence of bases in your genome.
True or False? If false correct the statement.
23.
__________________ All somatic cells contain the same genes.
24.
___________________ All somatic cells express the same genes.
25.
____________________ Repressor proteins inhibit the transcription of genes
when those proteins are not needed.