* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Document
Vectors in gene therapy wikipedia , lookup
Segmental Duplication on the Human Y Chromosome wikipedia , lookup
Point mutation wikipedia , lookup
Comparative genomic hybridization wikipedia , lookup
Genome evolution wikipedia , lookup
Genomic library wikipedia , lookup
Extrachromosomal DNA wikipedia , lookup
History of genetic engineering wikipedia , lookup
Genomic imprinting wikipedia , lookup
Gene expression programming wikipedia , lookup
Skewed X-inactivation wikipedia , lookup
Genome (book) wikipedia , lookup
Epigenetics of human development wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Designer baby wikipedia , lookup
Polycomb Group Proteins and Cancer wikipedia , lookup
Hybrid (biology) wikipedia , lookup
Microevolution wikipedia , lookup
Y chromosome wikipedia , lookup
X-inactivation wikipedia , lookup
SC435 Genetics Seminar • Welcome to our Unit 3 Seminar • We will continue our discussion of heredity with a focus on the chromosomes • The seminar will begin at 9:00PM ET Unit 3 • • • • Discussion board Unit 3 Quiz Unit 3 Exam Final Project Topic Submission • Looking Ahead – Unit 4 • Assignment (2-3 page paper) UNIT 3 KEY CONCEPTS • Chromosomes in eukaryotic cells are usually present in pairs. • The chromosomes of each pair separate in meiosis, one going to each gamete. • In meiosis, the chromosomes of different pairs undergo independent assortment. • Chromosomes consist largely of DNA combined with histone proteins. • In many animals, sex is determined by a special pair of chromosomes, the X and Y. • Irregularities in the inheritance of an X-linked gene in Drosophila gave experimental proof of the chromosomal theory of heredity. Chromatin Structure • Each core particle consists of an octamere of pairs each of histone H2A, H2B, H3, and H4; a segment of DNA containing about 145 base pairs Fig. 3.15a Fig. 3.19 Chromosomes • The chromosomes in the nuclei of somatic cells are usually present in pairs. For example, humans have 23 pairs of chromosomes • Cells with nuclei of this sort, containing two similar sets of chromosomes, are called diploid 6 Chromosomes • The chromosome complement = the complete set of chromosomes of plants and animals • The nucleus of each somatic cell contains a fixed number of chromosomes typical of the particular species • The number of chromosomes vary tremendously among species and have little relationship to the complexity of the organism Fig. 3.2 Fig. 3.3 Mitosis: Interphase, Late prophase, Metaphase Fig. 3.3 Mitosis: Anaphase and Telophase Fig. 3.3 Mitosis Meiosis • Meiosis is a mode of cell division in which cells are created that contain only one member of each pair of chromosomes • Meiosis consists of two successive nuclear divisions • Meiosis results in four daughter cells, each genetically different and each containing one haploid set of chromosomes • Meiosis is a more complex and considerably longer process than mitosis and usually requires days or even weeks Meiosis • The oocytes form egg cells and the spermatocytes form sperm cells • In the females of both animals and plants, only one of the four products develops into a functional cell (the other three disintegrate) Fig. 3.5 Outline of Meiosis • Prior to the first nuclear division, the members of each pair of chromosomes become closely associated along their length • The chromosomes that pair with each other are said to be homologous chromosomes • Each member of a pair of homologs consists of a duplex of two sister chromatids joined at the centromere. The pairing of the homologous chromosomes therefore produces a four-stranded structure Outline of Meiosis • At the time of pairing, the homologs can exchange genes which results in chromosomes that consist of segments from one homolog intermixed with segments from the other • In the first nuclear division, the homologous chromosomes are separated from each other, one member of each pair going to opposite poles of the spindle • Two nuclei are formed, each containing a haploid set of duplex chromosomes Outline of Meiosis • The second nuclear division resembles a mitotic division, but there is no DNA replication. • At metaphase, the chromosomes align on the metaphase plate, and at anaphase, the chromatids are separated into opposite daughter nuclei • The net effect of the two divisions is the creation of four haploid nuclei, each containing the equivalent of a single sister chromatid from each pair of homologous chromosomes Mitosis vs. Meiosis • Meiosis produces four cells: each contains one copy of each pair of homologous chromosomes = genetically haploid • Mitosis produces two cells which contain both members of each pair of homologous chromosomes = genetically diploid Meiosis: Prophase I • Leptotene - the chromosomes first become visible as long, thread-like structures • Zygotene - synapsis of homologous chromosomes = bivalent • Pachytene - crossing-over between homologs • Diplotene - chromosome repulsion, however, they remain held together by cross-connections resulting from crossing-over. Each crossconnection, called a chiasma is formed by a breakage and rejoining between nonsister chromatids Fig. 3.9b Meiosis II • The second meiotic division (meiosis II) is called the equational division because the chromosome number remains the same in each cell before and after the second division • In some species, the chromosomes pass directly from telophase I to prophase II without loss of condensation • After a short prophase II and the formation of seconddivision spindles, the centromeres of the chromosomes in each nucleus become aligned on the central plane of the spindle at metaphase II Meiosis II • In anaphase II, the centromeres divide and the chromatids of each chromosome move to opposite poles of the spindle • Once the centromere has split at anaphase II, each chromatid is considered a separate chromosome • Telophase II is a transition to the interphase condition of the chromosomes in the four haploid nuclei, accompanied by division of the cytoplasm. Meiosis • The chromatids of a chromosome are usually not genetically identical because of crossingover associated with the formation of chiasmata during prophase of the first division Chromatin Structure • Nucleosomes coil to form higher order DNA structure called the 30-nm chromatin fiber • In the nucleus of a nondividing cell, chromatin fibers form discrete chromosome territories • Chromosome territories are correlated with gene densities • Territories of chromosome domains that are relatively gene rich tend to be located toward the interior of the nucleus Fig. 3.21 (Micrograph courtesy of T.C. Hsu and Sen Pathak) Nondisjunction • Experimental proof of the chromosome theory of heredity came from nondisjunction • Nondisjunction = chromosomes fail to separate (disjoin) and move to opposite poles of the division spindle, results in loss or gain of a chromosome • Calvin Bridges demonstrated that exceptional behavior of chromosomes is precisely paralleled by exceptional inheritance of their genes X-Linked Inheritance • Special chromosomes determine sex in many organisms • X and Y chromosomes = sex chromosomes which are non-identical but share some genes • In most organisms, the Y chromosome carries few genes other than those related to male determination • X-linked genes are inherited according to sex • Hemophilia is a classic example of human Xlinked inheritance