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Nasonia vitripenni You are the PI (Primary Investigator) Jewel Wasp Tell me about it… Life Cycles Haplo/Diplo Genetics Wasp Development Timelines o o 18 , 28 C Complementation Process by which an allele of each of two mutant genes makes up for the defect in the other gene. You Find Three Mutants With a Similar Phenotype Are they the Same Genotype? Possible Physiology Enzyme A Enzyme A Enzyme A Enzyme A Enzyme B Enzyme B Enzyme B Enzyme B transport Enzyme C Enzyme C Enzyme C Enzyme C Complement a a | | | | B B Think about dominant genes! x a A | | | | B b A A | | | | b b Two genes Both Recessive Fail to Complement A A | | | | b b x A A | | | | b b A A | | | | b b 1 gene Three scarlet eyed mutants, how many loci? stDR, stC, st318 stDR For example. stC Connect same gene with a line. st318 Handling Wasps Sexing Wasps adults Sexing Wasps larvae, tuxedo – black stages Sex Pictures Scoring Red Eyes Here Scoring Eye Color Another Chart, Same Info Wasp Business Link To Do Plan experiment! Thursday define symbols, diagram crosses, determine expectations, etc. To Do Tuesday? Each student: complementation. Per bench, or per pair (will tell tomorrow), recessive, dominance, controls (stock maintenance). DR C 318 st , st , st F1 data, F2 data that means something Tinker with wasps, Next Week Exam Review Exam I, Basic Mendel! Basic Mendel! Basic Mendel! Basic Mendel! Basic Mendel!