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VC221: Tomato
sequencing
Key for pachytene chromosome identification
1st criteria: Chromosome with NOR vs without with: chromosome 2
2nd criteria: Position of the centromere
a) Median chromosomes 5, 11, 12 (metacentric)
b) Sub median all others (sub-metacentric)
3rd criteria: Short arm heterochromatic regions
a) A conspicuous cluster of chromomeres compressed together:
chromosomes. 3, 4, 6.
b) More than 1 cluster of chromomeres: chromosomes. 1, 7, 10.
Primary trisomic
Pachytene and diakinesis/metaphase I: 12II+ 1I, 11II +1III
Secondary trisomic
Pachytene: Isochromosome paired with itself, triradial III
Diakinesis/Met I: 11II +ring of III, 12II + 1I (mini ring).
Tertiary trisomic
Diakinesis/Met I: 10II + 1V
Telo trisomic
Diakinesis/Met I: heteromorphic III, or tiny univalent
Primary Monosomic
Diakinesis/Met I: 11II + 1I
Tertiary Monosomic
Diakinesis/Met I: 10II + 1III, 10II + 3I
Tomato Mapping Stocks
LA 1182 sy - sf(3) alb - mua (12)
LA1441 coa - c(6) a - hl (11)
LA1443 scf - dgt(1) l -al(8)
LA1444 wd - d(2) af - tf(5)
LA1445 clau-di(4) icn - ag(10)
LA1446 var - not(7) ah - marm(9)
Distribution of tomato species
Zuriaga et al 2008
L. cheesmanii minor
typicum
(based on 6 DNA seq, Adh 2, fw2.2)
S. lycopersicon
Eulycopersicon
(color-fruited)
typ: S. cheesmaniae
min: S. galapagence
S. pimpinellifolium
S. neorickii
S. chemieleskii
Eriopersicon
Green-fruited
S. habrochaites
S. pennellii
S. peruvianum
S. arcanum
S. Corneliomuellerii
S. huayalasense
S. chilense
S. lycopersicoides, S. ochrantum, S. sitiens
Zuriaga et al 2008
Dimeric
Monomeric
Esc-pennelli introgression lines
Each stock is homozygous for a single chromosome segment (delineated by RFLP markers) introgressed from L.
pennellii, such that the entire wild species genome is represented in a group of 50 lines.
Dissection of QTL se2.1(stigma
exertion
• Four loci influencing stamen length (three
closely linked) were identified.
• A single locus for style length was identified (the
long style allele was shown to be dominant
using esc-penn sustitution lines).
• A single locus for anther dehiscence was also
found in the region.
Chen and Tanksley 2004
Fine mapping loci contributing
to the se2.1 QTL
• Five closely linked genes all contribute to
stigma exsertion phenotype.
• This may be the remnant of a “co adapted
gene complex” where linked genes
cooperatively contribute to an adaptation,
and are inherited as a single unit.
Tomato Genome Sequencing Project
Tomato SI
Successful cross
Unsuccessful cross
SeSe (female) x
S1S2(male)
S1S2 (female)
x SeSe(male)
SeSe(female) x
SPSP(male)
SPSP(female) x SeSe
(male)
SPSP (female) x S1S2
(male)
S1S2 (female) x SPSP
(male)