Download Genes involved in plant stress response and their

Genes Involved in Plant Stress
Response and Evolution of their
Responsiveness
Cheng Zou
11/09/2007
The Stress Response is Unique and Vital to Plants
Soybean aphid, Aphis glycines (Photo Claudio Gratton)
Different from animals, when exposed to adverse environments, plant can not run away. So
along evolution plant developed a specific system to fight against them. There are two main
sources of stress, one is biotic, such as invasion of bacterium, fungi; another one is abiotic,
such as cold, drought and heat.
Based on the AtGenExpress experiments in Arabidopsis, of 22110 genes which are
presented on the micro array, 37% genes are up or down regulated significantly by at least
one stress condition. So we are interested in what kind of genes tend to have stress
responsiveness and how gene function in stress response evolved
Arabidopsis Expression profile under stress condition
Type of stress
Osmotic
UV-B
Wounding
Cold4C
Abiotic stress
Heat
Genotoxic
Drought
Salt
2 or 3 replicates
control
Oxidative
Flg22
For each condition, Up- and down-regulated genes were
defined as genes with significantly higher and lower
intensities (at 5% false discovery rate) with treated samples
than those in controls. Non-responsive genes were defined
GST-NPP1
as genes that were not significantly up- or down-regulated.
avrRpm1
DC3000
Biotic stress
HrcCHrpZ
LPS
P-infestans
Psph
Number of Up or Down regulated gene under each
condition
Wounding_12
Wounding_3
Wounding_1
Wounding_0.5
Wounding_0.2
UV-B_24
UV-B_12
UV-B_6
UV-B_3
UV-B_1
UV-B_0.25
Salt_24
Salt_12
Salt_6
Salt_3
Osmotic_24
Osmotic_12
Osmotic_6
Osmotic_3
Osmotic_1
H eat_3+9
H eat_3+3
H eat_3+21
H eat_3+1
H eat_3
H eat_1
H eat_0.5
H eat_0.25
Genotoxic_12
Genotoxic_6
D rought_6
D rought_3
Cold4C_24
Cold4C_12
Psph_24
Psph_6
Psph_2
P.infestans_24
P.infestans_12
P.infestans_6
H rpZ _4
H rpZ _1
H rcC-_24
H rcC-_6
H rcC-_2
GST -NPP1_4
GST -NPP1_1
Flg22_4
Flg22_1
D C3000_24
D C3000_6
D C3000_2
av rRpm1_24
av rRpm1_6
av rRpm1_2
0
500
1000
1500
2000
up
2500
dn
3000
3500
4000
0
500
1000
1500
up
2000
dn
2500
3000
3500
the Breadth of Stress Response of All the Genes
Up Regulation of Abiotic Stress
Up Regulation of Biotic Stress
Fig1.The observed distribution of the breadth of stress response ( red dash line) was compared to
random distributions calculated (box plot), by assuming that for each condition there are same number of
up regulated genes and regardless interactions among different conditions. *Asterisk indicates that the
differences is significant at < 0.001% level.
Q1:What kind of families are overrepresented in up-refulated genes?
the Breadth of Stress Response of Domain Family
Up Regulation of Abiotic Stress
Up Regulation of Biotic Stress
Fig2.The observed distribution of the breadth of domain (dash line) was compared to random
distributions calculated (box plot), by assuming that for each condition there are same number of domain
families that over or underrepresented in up regulated genes, and regardless interactions among different
conditions. *Asterisk indicates that the differences is significant at < 0.001% level.
Domain families that over or under represented in up
regulated genes
Abiotic
biotic
stress
stress
Evolution of Stress Response