Download Slide () - Journal of Speech, Language, and Hearing Research

From: Biotechnology in the Treatment of Sensorineural Hearing Loss: Foundations and Future of Hair Cell
Regeneration
J Speech Lang Hear Res. 2011;54(6):1709-1731. doi:10.1044/1092-4388(2011/10-0149)
Legend:
Gene delivery in hearing research. Panel 1: Viral-mediated gene delivery utilizes virus particles to deliver a gene-of-interest into a cell. A: A virus
(blue) attaches to a cell (see Subpanel B). Once attached, the DNA of the virus (see black vertical line) will be inserted into the cytoplasm of the
host cell (see Subpanels 1C and 1D), where the viral DNA will incorporate into the DNA of the host cell (red; see Subpanel 1E). Viral DNA
consists of self-replicating genes and will cause the host cell to manufacture more viral particles (see Subpanel 1F). Subpanel 1G: Eventually,
the host cell will release viral particles back into the
environment.
Subpanel
1H: In a viral-mediated gene delivery system, the viral DNA that
Copyright
© American
Speech-Languagecodes
for
self-replication
is
replaced
with
a
gene-of-interest,
such
as
atoh1.
Cells
Date of download: 5/13/2017
Hearing Association that are infected with the engineered virus will express the
atoh1.Panel 2:Izumikawa et al. (2005) injected an adenovirus that carried the atoh1 gene into the cochleas of chemically deafened adult guinea