Download ANIMAL CELL CULTURE

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Monolayer
Substrate attachment for growth
Charged substrate
Contact inhibition controls growth
Necessary for cells to spread or
divide(mitosis)
Determines the nature of the cell
All vertebrate cells
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Suspension cells
No substrate attachment for growth
No charged substrate
Density limitation controls growth
Neoplastic in nature
Cancerous cells, tumor cells, etc.
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Proteins located on cell surface
Involved in binding with other cells or
extracellular matrix
Transmembrane receptors
Calcium dependent CAMs
L-CAMs & N-CAMs
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Cell to matrix interaction
Signal transduction from the ECM to the cell
Ligands include fibronectin, vitronectin, colla
gen, and laminin
immune patrolling, cell migration, and
binding to cells by certain viruses
GPIIbIIIa, an integrin on the surface of
blood platelets responsible for attachment to
fibrin within a developing blood clot.
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class of type-1 transmembrane proteins
Homophilic Ca dependent glycoproteins
Cell to cell interaction
Forms adherens junctions for attachment
Polypeptides that undergo many post-translational modifications
to become the proteins which mediate cell-cell adhesion and
recognition
720–750 amino acids long
over 80 types of cadherins in humans have been identified and
sequenced
Behave as both receptors and ligands
responsible for the separation of the different tissue layers, and
for cellular migration
Concentrated at intermediate cell junctions
E-Cadherins , N-Cadherins , P-Cadherins
N-CAMs and L-CAMs
Transformed cells don’t attach to the substrate
Transformed cells may lack specific CAMs(e.g. LCAM).
 Effect of L-CAMs is suppressed
 Effect of N-CAMs is over expressed
 Degree of phosphorylation is also changed
 The loss of cell-cell recognition, a product of
reduced cell-cell adhesion, leads to disorganized
growth pattern and the loss of contact inhibition
of cell motility and density limitation of cell
proliferation.
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