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Kostelecky et al. 2006, Journal of Bacteriology The Crystal Structure of the Zinc Phosphodiesterase from Escherichia coli Provides Insight into Function and Cooperativity of tRNase Z-Family Proteins Protein: Ribonuclease bn Uniprot ID: P0A8V0 GO: 0008270 zinc ionbinding Evidence: IDA Fig. 1a – In solution, zinc ions bind to two locations of the two subunits, in the active site of each Fig. 1b – This allows for the binding of two amino acids (yellow) and zinc ligands (blue), preparing the protein for cleavage of pre-tRNA.