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Unit 5 Infectious Disease Lesson 5.1 Infection How are infectious diseases spread through a population? Bacteria and Viruses: Fungus: Spread by eating contaminated food or by vectors, like mosquitoes and malaria Helminthes: Grow in warm/damp areas and can spread through spores in the air, skin to skin contact, or sharing unwashed clothes Protozoa: Most common infectious agents Bacteria are prokaryotic organisms that can survive on their own Virus are not cellular and need a host to reproduce Spread from contact with a contaminated person or thing and through the air Parasitic worms that are transmitted through under cooked meats and eating fecal matter Prions: Mutated proteins that can happen randomly or by infected human tissue What is aseptic technique? These are techniques used to maintain a sterile environment when working with bacteria. Important to reduce spread of bacteria and contamination Aseptic techniques include Wearing goggles, gloves, and aprons/lab coats Using Bleach solution to clear your area before and after use Keep the petri dish closed as much as possible Use a flame to sterilize slides and loops before use Wash hands and forearms How can an unknown sample of bacteria be identified? Colony Morphology Grow bacteria on Petri Dishes in isolated colonies Analyze the specific characteristics of colony growth to help determine bacteria species Gram Staining Stain bacteria first with grams stain and then safranin. Gram + will stain purple and Gram – will stain pink Chemical Tests A variety of chemical tests can be used to help identify bacteria since each species reacts differently to certain tests. How can an unknown sample of bacteria be identified? Gram Staining Continued Gram + have thick peptidoglycan wall that absorbs purple stain Gram – have thin peptidoglycan wall with an outer lipid membrane. The lipid membrane washes off and then the cell wall is stained pink Bacteria shape Gram staining allows us to see the shape on the individual bacteria Coccus – Circle Bacilli – Rods Spirilla - Spiral How does the immune system function to protect the human body from foreign invaders? Nonspecific Defense Defense mechanisms against all types of foreign invaders They do not remember or focus on a specific type of invader First Line Skin – protective layer against foreign objects Mucus – Lines lungs and other inner surfaces that traps and helps expel foreign objects Nose Hair – Filters air and traps foreign particles How does the immune system function to protect the human body from foreign invaders? Nonspecific Defense Second Line Inflammation – Blood rushes to the area bringing phagocytes and other white blood cells to the area. Also helps to retain the infection in one area Phagocytes – have receptors that seek out pathogens (bad foreign invaders) and once bound to it the phagocyte eats the pathogen. Phagocytes can also lead into specific defense by presenting the antigen How does the immune system function to protect the human body from foreign invaders? Specific Defense Defense against specific invaders through the use of antigens and antibodies This comes into play after the invader gets past the nonspecific defense Two main cells are T- cells and B-cells How does the immune system function to protect the human body from foreign invaders? Specific Defense Antigens are proteins on the pathogens that tells human cells what they are. The phagocyte presents the antigen to helper T-cells that will produce proteins to activate B-cells How does the immune system function to protect the human body from foreign invaders? Specific Defense B-cells are activated by T-cells and also connects to pathogen antigens B-cells produce plasma cells that make antibodies specific to the antigen. The antibodies attach to the antigen and mark it for phagocytes to destroy. B-cells also produce memory cells that will “remember” same antigen so if the pathogen invades again the immune response will be faster