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I. Movement of Materials Through the Cell Membrane A. Concentration gradient 1. Molecular movement: molecules are constantly colliding, moving down a concentration gradient from HIGH to LOW,(trying to get to area of lower concentration so they can move and not collide, pioneers moved from E to W) 2. Concentration gradient: difference btwn the number of molecules inside vs. outside the cell 3. Dynamic equilibrium: NO difference in the # of molecules on either side of the membrane 4. *Hypertonic solution* has a higher concentration of dissolved particles than inside the cell, water mostly OUT (ocean water to human cells or fresh water fish in salt water, salt on slugs) 5. *Hypotonic solution* has a lower concentration of dissolved particles than inside the cell, water mostly IN (distilled water to human cells or salt water fish in fresh water) 6. *Isotonic solution* has an equal concentration of dissolved particles as the inside of a cell, water flows in and out at the same rate. (paramecium in fresh water, has contractile vacuole to expel excess water) 8. cytolysis- turgor increases, cell gets larger as it takes in water 9. plasmolysis- turgor decreases, cell gets smaller as it loses water Hypertonic Solution Isotonic Solution more water out water in & out at same rate Hypotonic Solution more water in B. Diffusion or Passive Transport NO ENERGY REQUIRED 1. net movement of the same molecules down a concentration gradient from *HIGH to LOW* a. *Osmosis*: diffusion of water across a semi-permeable membrane from HIGH to LOW b. *Facilitated diffusion*: molecules allowed or helped across the membrane by channel proteins 2. Rate is affected by *temperature, pressure, concentration of solution, size of molecules* C. Active transport ENERGY REQUIRED because a part of the cell is moving 1. movement of molecules against the concentration gradient using energy, *LOW to HIGH* 2. protein pumps in the membrane transport certain ions and large molecules across membranes 3. membrane folding or bulk tansport a. *Endocytosis* items into a cell; white blood cells engulf invaders i. *phagocytosis*: taking in food or solid particles ii. *pinocytosis*: taking in small amounts of liquid (paramecium/contractile vacuole) b. *Exocytosis* removal of particles from a cell REMEMBER: Water, oxygen, carbon dioxide, and amino acids move freely across the membrane b/c they’re small molecules. Proteins, carbohydrates, & ions need help from protein pumps b/c they’re bigger (ions because they’re charged)! II. Movement of Materials using the 2 types of Carrier Proteins **Transport Proteins = Carrier Proteins!! III. Flow Chart: Overview of Movement of Materials Transport Overview and Practice: Identify the processes described below by giving the specific type of movement involved. Look at the concentration gradient {concentration inside and outside of the cell} to help you decide!!!!! 1. Carrier proteins transport glucose 2. Sodium ions (Na+) are pumped out of into a muscle cell. *facilitated diffusion* a red blood cell. *active transport* 3. Fresh water moves into a unicellular organism (paramecium). *osmosis* 4. An amoeba engulfs a food particle. *phagocytosis* 5. Pockets of cell membrane fill with water and pinch off to become vacuoles in the cell. *pinocytosis* Name the following types of transport in the diagram: 6. *facilitated diffusion* 7. *diffusion* 8. *diffusion* 9. *phagocytosis/endocytosis* 10. *pinocytosis/endocytosis* 11. *osmosis* 12. *exocytosis* 13. *active transport*