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www.abcam.com Product Datasheet for ab15830 SOX2 antibody - Embryonic Stem Cell Marker Primary antibodies Rabbit polyclonal to SOX2 - Embryonic Stem Cell Marker Synthetic peptide derived from residues 1 - 100 of Human SOX2. Reacts with Human and Mouse. Predicted to react with Chicken (100% identity with immunogen), Xenopus laevis (91% identity with immunogen), Zebrafish (90% identity with Species Reactivity immunogen), Fugu (Pufferfish) (91% identity with immunogen) and Sheep (100% identity with immunogen) due to sequence homology. Not yet tested in other species. Tested Western blot, Immunofluorescence applications Recommended dilutions IF: Use at a concentration of 3 ƒÊg/ml. Application notes WB: Use at a concentration of 0.3 ƒÊg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa). Product Name Product type Description Immunogen Abcam's AbpromiseSM Quality is important to Abcam. If SOX2 antibody - Embryonic Stem Cell Marker (ab15830) does not perform as described on this datasheet, notify us within 90 days of delivery using this form, so that we can help you or offer you a replacement or a refund. Cellular localisation Research areas Relevance Nuclear Stem Cells >> Embryonic Germ Cells Stem Cells >> Embryonic Stem Cells >> Intracellular Nuclear Signaling >> Transcription >> Domain Families >> HMG Box Neuroscience >> Growth & Development >> Signal Transduction Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper Neuroscience >> Neuronal Markers >> Neural Stem Cells SOX 2 is also known as SRY related HMG BOX gene 2. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX1, SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse. These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution. Alternative names for SOX2 antibody - Embryonic Stem Cell Marker (ab15830) Entrez Gene Database links GeneCard Omim Raised in Clonality Isotype Purity 396105 (Chicken) 6657 (Human) SwissProt 20674 (Mouse) 378723 (Zebrafish) GC03P182831 (Human) Unigene 184429 (Human) P48430 (Chicken) P48431 (Human) P48432 (Mouse) P54231 (Sheep) 601 (Chicken) 816 (Human) 4541 (Mouse) Rabbit Polyclonal IgG Immunogen affinity purified Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS. pH 7.4 Storage buffer Form Concentration Storage instructions Material safety datasheet (MSDS) for this product: Sodium Azide MSDS Liquid Batch dependent within range: 0.40 - 0.50 mg/ml Aliquot and store at -20 ‹C or -80 ‹C. Avoid repeated freeze / thaw cycles. At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SOX2 antibody - Embryonic Stem Cell Marker is on this datasheet. But please do contact us if you would like any reassurance! See below for SOX2 antibody - Embryonic Stem Cell Marker images, references, products related to ab15830 and other tools. SOX2 antibody - Embryonic Stem Cell Marker images: Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830) Sox2-specific antibody, ab15830, stained the nucleus of human ES cells (H9 line). Upon differentiation into neurones ab15830, but not Oct4-specific antibody, stained the nuclei (data not shown). Mouse embryonic fibroblasts were not stained by ab15830. Dilution used: 5µg/ml. Ludovic Vallier, University of Cambridge - SOX2 antibody - Embryonic Stem Cell Marker (ab15830) Lanes 1 - 2 : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 1 µg/ml Lane 1: 20 µg Mouse embryonic stem cell lysate Lane 1: 20 µg Human embryonic stem cell lysate ab15830 recognised a band of the expected size (34 kDa) in human and mouse embryonic stem cell lysates. Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab15830) All lanes : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 0.3 ug/ml Lane 1 : Mouse Embryonic Stem Cell lysate Lane 2 : Mouse Embryonic Germ Cell lysate Lane 3 : Mouse Embryonic Fibroblast Lysate Predicted band size : 34kDa Observed band size : 37kDa (why is the actual band size different from the predicted?) Petra Hajkova, University of Cambridge SOX2 antibody - Embryonic Stem Cell Marker - related products: Products for Immunofluorescence Subcellular Markers Products for Western blot Loading controls Lysates Immunizing Peptide for SOX2 antibody - Embryonic Stem Cell Marker ab15831 SOX2 peptide Compatible Secondaries for SOX2 antibody - Embryonic Stem Cell Marker ab2892 Goat polyclonal to mouse (AP) + rabbit (HRP) IgG double stain - predil ab6564 Cy5 antibody ab6717 FITC antibody ab6720 Biotin antibody ab6721 HRP antibody ab6722 Alkaline Phosphatase antibody ab6793 ab7007 ab7019 Texas Red antibody Phycoerythrin antibody Agarose antibody Other SOX2 Antibodies More SOX2 antibodies(1)... All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"