Download Product Datasheet for ab15830

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Product Datasheet for
ab15830
SOX2 antibody - Embryonic Stem Cell Marker
Primary antibodies
Rabbit polyclonal to SOX2 - Embryonic Stem Cell Marker
Synthetic peptide derived from residues 1 - 100 of Human SOX2.
Reacts with Human and Mouse.
Predicted to react with Chicken (100% identity with immunogen), Xenopus
laevis (91% identity with immunogen), Zebrafish (90% identity with
Species Reactivity
immunogen), Fugu (Pufferfish) (91% identity with immunogen) and Sheep
(100% identity with immunogen) due to sequence homology.
Not yet tested in other species.
Tested
Western blot, Immunofluorescence
applications
Recommended dilutions
IF: Use at a concentration of 3 ƒÊg/ml.
Application notes
WB: Use at a concentration of 0.3 ƒÊg/ml. Detects a band of approximately
37 kDa (predicted molecular weight: 34 kDa).
Product Name
Product type
Description
Immunogen
Abcam's AbpromiseSM
Quality is important to Abcam. If SOX2 antibody - Embryonic
Stem Cell Marker (ab15830) does not perform as described on
this datasheet, notify us within 90 days of delivery using this
form, so that we can help you or offer you a replacement or a
refund.
Cellular
localisation
Research areas
Relevance
Nuclear
Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Neuroscience >> Growth & Development >> Signal Transduction
Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine
Zipper >> Leucine Zipper
Neuroscience >> Neuronal Markers >> Neural Stem Cells
SOX 2 is also known as SRY related HMG BOX gene 2. All SOX proteins
have a single HMG box and bind linear DNA in a sequence-specific manner,
resulting in the bending of DNA through large angles. Bending causes the
DNA helix to open for some distance, which may affect binding and
interactions of other transcription factors. SOX1, SOX2 and SOX3 show the
closest homology to SRY. They share maximum homology within the HMG
domain and are expressed mainly in the developing nervous system of the
mouse. These genes share significant homology outside the HMG box also
and are highly conserved throughout their evolution.
Alternative names for SOX2 antibody - Embryonic Stem Cell Marker
(ab15830)
Entrez
Gene
Database links
GeneCard
Omim
Raised in
Clonality
Isotype
Purity
396105 (Chicken)
6657 (Human)
SwissProt
20674 (Mouse)
378723 (Zebrafish)
GC03P182831 (Human)
Unigene
184429 (Human)
P48430 (Chicken)
P48431 (Human)
P48432 (Mouse)
P54231 (Sheep)
601 (Chicken)
816 (Human)
4541 (Mouse)
Rabbit
Polyclonal
IgG
Immunogen affinity purified
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Storage buffer
Form
Concentration
Storage
instructions
Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
Liquid
Batch dependent within range: 0.40 - 0.50 mg/ml
Aliquot and store at -20 ‹C or -80 ‹C. Avoid repeated freeze / thaw cycles.
At Abcam, we have one centralized database to hold all of our
product information, so that everything we know about this SOX2
antibody - Embryonic Stem Cell Marker is on this datasheet. But
please do contact us if you would like any reassurance!
See below for SOX2 antibody - Embryonic Stem Cell Marker images, references, products related
to ab15830 and other tools.
SOX2 antibody - Embryonic Stem Cell Marker images:
Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
Sox2-specific antibody, ab15830, stained the nucleus of human ES cells (H9 line). Upon
differentiation into neurones ab15830, but not Oct4-specific antibody, stained the nuclei (data not
shown). Mouse embryonic fibroblasts were not stained by ab15830.
Dilution used: 5µg/ml.
Ludovic Vallier, University of Cambridge
- SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
Lanes 1 - 2 : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 1 µg/ml
Lane 1: 20 µg Mouse embryonic stem cell lysate
Lane 1: 20 µg Human embryonic stem cell lysate
ab15830 recognised a band of the expected size (34 kDa) in human and mouse embryonic stem
cell lysates.
Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
All lanes : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 0.3 ug/ml
Lane 1 : Mouse Embryonic Stem Cell lysate
Lane 2 : Mouse Embryonic Germ Cell lysate
Lane 3 : Mouse Embryonic Fibroblast Lysate
Predicted band size : 34kDa
Observed band size : 37kDa (why is the actual band size different from the predicted?)
Petra Hajkova, University of Cambridge
SOX2 antibody - Embryonic Stem Cell Marker - related
products:
Products for Immunofluorescence
Subcellular Markers
Products for Western blot
Loading controls
Lysates
Immunizing Peptide for SOX2 antibody - Embryonic Stem Cell Marker
ab15831 SOX2 peptide
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ab7007
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR
DIAGNOSTIC OR THERAPEUTIC USE"