Download Cytokine Microarray Project

Cytokines and Cytokine Dependent Signalling Pathways as Therapeutic
Target Structure using an Inflammation microarray
Christian Betz, Ocimum Biosolutions
Prof. Michael Kracht, Medical School Hanover
Together with the Medical School Hanover, Germany we developed oligonucleotidebased topic microarrays for inflammation studies (Human/Mouse). The arrays
comprise of 135 regulated genes most relevant to various inflammatory processes.
The eligibility of oligos representing these genes was validated by RT-PCR. Final
validation of the human and murine probes was performed in 214 and 87
hybridizations, respectively.
The use of this microarray in a cytokine microarray project “Cytokines and Cytokine
Dependent Signalling Pathways as Therapeutic Target Structures”, hosted by the
Institute of Pharmacology, Medical School Hanover, will be presented.
The aim of this project is to develop, apply and adapt DNA microarray technology
for quantitative and kinetic measurements of cytokine-induced gene expression in cell
culture systems, transgenic mice, animal models of disease and in human patients that
are studied by members of the SFB566. CytoBase, a freely accessible database
collecting the microarray results of these experiments will be described.