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Unit C—Cycling of Matter in Living Systems Unit Support Line M ast er 5 Name: _____________________________________ Development of the Cell Theory and Imaging Technologies Contributor Technology or Technique Achievement Ancient Greeks Viewing through a water-filled Observed magnification, but did glass sphere not put it to practical use Hans and Zacharias Jansen Two lens system Magnification extending the reach of the human eye Robert Hooke Three lens system Magnification allowing for the observation of cells Antoni van Leeuwenhoek Single lens system similar to a magnifying glass Able to see moving microorganisms Compound Light Microscope Uses two or more curved lenses and a light source to magnify objects Louis Pasteur Used flasks with various neck shapes to manipulate the access of dust to broth Provided strong evidence disputing spontaneous generation Robert Brown Microscope with a single lens Identified the nucleus and suggested its importance in cells Development of the cell theory M.J. Schleiden, T.S. Schwann, and Rudolf Virchow Microscope studies: – of plant tissues – of animal tissues – of diseased tissues Stain Provides contrast for observing individual structures. Kills cells. Resolution Ability to distinguish between two structures that are very close together Fluorescence Microscopy Provides information about molecules on the cell surface Confocal Technology Uses laser beams; the living, transparent cell can be viewed in three-dimensions Electron Microscopy Uses a beam of electrons to produce images with fine detail. Addison Wesley Science 10 7 Copyright © 2004 Pearson Education Canada Inc.