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Unit C—Cycling of Matter in Living Systems
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Name: _____________________________________
Development of the Cell Theory and Imaging Technologies
Contributor
Technology or Technique
Achievement
Ancient Greeks
Viewing through a water-filled Observed magnification, but did
glass sphere
not put it to practical use
Hans and Zacharias Jansen
Two lens system
Magnification extending the
reach of the human eye
Robert Hooke
Three lens system
Magnification allowing for the
observation of cells
Antoni van Leeuwenhoek
Single lens system similar to a
magnifying glass
Able to see moving microorganisms
Compound Light Microscope
Uses two or more curved lenses
and a light source to magnify
objects
Louis Pasteur
Used flasks with various neck
shapes to manipulate the
access of dust to broth
Provided strong evidence
disputing spontaneous generation
Robert Brown
Microscope with a single lens
Identified the nucleus and
suggested its importance in cells
Development of the cell theory
M.J. Schleiden,
T.S. Schwann, and
Rudolf Virchow
Microscope studies:
– of plant tissues
– of animal tissues
– of diseased tissues
Stain
Provides contrast for observing
individual structures. Kills cells.
Resolution
Ability to distinguish between
two structures that are very close
together
Fluorescence Microscopy
Provides information about
molecules on the cell surface
Confocal Technology
Uses laser beams; the living,
transparent cell can be viewed in
three-dimensions
Electron Microscopy
Uses a beam of electrons to
produce images with fine detail.
Addison Wesley Science 10
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