Download Polyclonal Antibodies to Lamins - Edinburgh Research and Innovation

Polyclonal Antibodies to Lamins These rabbit polyclonal antibodies, raised against the chromatin‐
binding domains of the human Lamin proteins A/C, B1 and B2, give strong signals with low background and are, therefore, ideal for immunofluorescence applications. Lamins are membrane proteins that provide a structural framework for the nucleus and are also essential for maintaining normal cell functions, such as cell cycle control, DNA replication and chromatin organization. Mutations in the genes encoding nuclear Lamins are implicated in a number of diseases (laminopothies), including Emery‐
Dreifuss muscular Dystrophy and a number of premature ageing syndromes. Most currently available antibodies to Lamin proteins are made against non‐ideal epitopes. They are either to the Rod domain which is highly conserved, making the antibodies lack specificity among subtypes, or they are to the C terminal globular domain region of the proteins ‐ a region which has multiple binding partners and is, therefore, often masked. These antibodies have been made to the chromatin binding region of the Lamin proteins – a region which is the least masked at the nuclear envelope. Consequently, they are highly specific and give clear strong signals in immunofluorescence applications. The A‐type Lamins comprise a set of proteins arising from the same gene by alternative splicing ‐ principally Lamin A, Lamin C and Lamin A∆10. The Lamin A/C antibody is to a shared domain so recognises all known splice variants. The B‐type Lamins include two proteins arising from two distinct genes Lamin B1 and Lamin B2. Many Lamin B antibodies fail to distinguish between these gene products, but there is no cross‐
reactivity between the Lamin B1 and Lamin B2 antibodies presented here. 
Highly specific polyclonal antibodies 
Strong signal and low background in Immunofluorescence applications 
Recognise all splice variants of their respective Lamin genes Publication Schirmer et al. (2001). Involvement of the Lamin Rod Domain in Heterotypic Lamin Interactions Important for Nuclear Organization. Journal of Cell Biology, Vol. 153, No. 3, 479‐489 Commercial Offering The University of Edinburgh is seeking enquires from commercial organisations to in‐licence the technology to sell the polyclonal antibody for research purposes. Order it now! This University of Edinburgh technology can be licensed directly under a standard non‐exclusive license agreement at: https://licensing.eri.ed.ac.uk Contact Shona Cunningham Applications 
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Benefits Immunofluorescence Western Blotting Telephone: +44 (0)131 650 6430 Email: [email protected] www.research‐innovation.ed.ac.uk