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Download PE anti-mouse RAE-1δ Antibody
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Version: 1 Revision Date: 11/30/2012 PE anti-mouse RAE-1δ Antibody Catalog# / Size 133203 / 50 µg Clone Charlotte d1.23 (See other available formats) Other Names Retinoid acid early inducible gene-1δ, RAE-1δ, RAE-1δ Isotype Mouse IgG1 Ave. Rating ★★★★★ 0 reviews Description RAE-1δ is one of the five RAE-1 family, GPI-linked membrane protein consisting of alpha, beta, gamma, delta, and epsilon. They are strong homology within the family, related by 92%-95% sequence identity. They are distantly related to MHC class I proteins. RAE-1 proteins are abundantly expressed in fetal tissues, but not in normal adult tissue. They are constitutively expressed on some tumors and can be induced by retinoid acid. Its ligand NKG2D is found on NK cells, many transformed cell lines, and LPS-stimulated peritoneal macrophages. The interaction of NKG2D with RAE-1 transmits stimulatory signals to NK cells and other hematopoietic cells, leading to enhanced proliferation, cytokine secretion and target killing. They are involved in the regulation of innate and immune cytotoxic responses to tumors, pathogen-infected cells, and autoimmune diseases. Reactivity Mouse RAE-1δ transfected cells stained with Charlotte d1.23 PE See PE spectral data Immunogen Mouse RAE-1δ transfected RMA-S cell line Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody. Concentration 0.2 mg/ml Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application FC - Quality tested Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.015 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Excitation Laser Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm) Application References 1. Lodoen M, et al. 2003. J. Exp. Med. 197:1245 2. Carayannopoulos LN, et al. 2002. Eur J. Immunol.32 (3):597 (PubMed link indicates BioLegend citation) RRID Publication Library AB_1595625 (BioLegend Cat. No. 133203) Antigen Details Structure RAE is a family of GPI-linked membrane protein consisting of alpha, beta, gamma, delta, and epsilon. They are strong homology within the family, related by 92%-95% sequence identity. They are distantly related to MHC class I proteins. Distribution RAE-1 proteins are abundantly expressed in fetal tissues, but not in normal adult tissue. They are constitutively expressed on some tumors and can be induced by retinoid acid. Ligand Receptor NKG2D Antigen References 1. Cerwenka A, et al. 2000. Immunity 12:721 2. Lodoen M, et al. 2003. J. Exp. Med. 197:1245 3. Diefenbach A, et al. 2001. Nature 413:165 Gene ID 66679 UniProt View information about RAE-1 delta on UniProt.org Documentation Technical data sheet Certificate of Analysis Safety Data Sheet Related Protocols Cell Surface Immunofluorescence Staining Protocol Related FAQs There are no FAQs for this product. Related Categories Immunology > Mouse Immunology > Innate Immunity > RAE-1δ > Charlotte d1.23 > Immunology > Mouse Immunology > NK/NKT Cells > RAE-1δ > Charlotte d1.23 > For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587