Download PE anti-mouse RAE-1δ Antibody

Version: 1
Revision Date: 11/30/2012
PE anti-mouse RAE-1δ Antibody
Catalog# / Size
133203 / 50 µg
Clone
Charlotte d1.23 (See other available formats)
Other Names
Retinoid acid early inducible gene-1δ, RAE-1δ, RAE-1δ
Isotype
Mouse IgG1
Ave. Rating
★★★★★ 0 reviews
Description
RAE-1δ is one of the five RAE-1 family, GPI-linked membrane protein
consisting of alpha, beta, gamma, delta, and epsilon. They are strong
homology within the family, related by 92%-95% sequence identity. They
are distantly related to MHC class I proteins. RAE-1 proteins are abundantly
expressed in fetal tissues, but not in normal adult tissue. They are
constitutively expressed on some tumors and can be induced by retinoid
acid. Its ligand NKG2D is found on NK cells, many transformed cell lines,
and LPS-stimulated peritoneal macrophages. The interaction of NKG2D
with RAE-1 transmits stimulatory signals to NK cells and other hematopoietic
cells, leading to enhanced proliferation, cytokine secretion and target
killing. They are involved in the regulation of innate and immune cytotoxic
responses to tumors, pathogen-infected cells, and autoimmune diseases.
Reactivity
Mouse
RAE-1δ transfected cells stained with
Charlotte d1.23 PE
See PE spectral data
Immunogen
Mouse RAE-1δ transfected RMA-S cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal
conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
Application
FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow
cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.015 µg
per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal
performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
1. Lodoen M, et al. 2003. J. Exp. Med. 197:1245
2. Carayannopoulos LN, et al. 2002. Eur J. Immunol.32 (3):597
(PubMed link indicates
BioLegend citation)
RRID
Publication Library
AB_1595625 (BioLegend Cat. No. 133203)
Antigen Details
Structure
RAE is a family of GPI-linked membrane protein consisting of alpha, beta, gamma, delta, and
epsilon. They are strong homology within the family, related by 92%-95% sequence identity.
They are distantly related to MHC class I proteins.
Distribution
RAE-1 proteins are abundantly expressed in fetal tissues, but not in normal adult tissue. They
are constitutively expressed on some tumors and can be induced by retinoid acid.
Ligand Receptor
NKG2D
Antigen References
1. Cerwenka A, et al. 2000. Immunity 12:721
2. Lodoen M, et al. 2003. J. Exp. Med. 197:1245
3. Diefenbach A, et al. 2001. Nature 413:165
Gene ID
66679
UniProt
View information about RAE-1 delta on UniProt.org
Documentation
Technical data sheet
Certificate of Analysis
Safety Data Sheet
Related Protocols
Cell Surface Immunofluorescence Staining Protocol
Related FAQs
There are no FAQs for this product.
Related Categories
Immunology > Mouse Immunology > Innate Immunity > RAE-1δ > Charlotte d1.23 >
Immunology > Mouse Immunology > NK/NKT Cells > RAE-1δ > Charlotte d1.23 >
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