Download PE anti-mouse CD153 Antibody

Version: 1
Revision Date: 11/30/2012
PE anti-mouse CD153 Antibody
Catalog# / Size
106405 / 50 µg
Clone
RM153 (See other available formats)
Other Names
CD30 ligand, CD30L, TNFSF8
Isotype
Rat IgG2b, κ
Ave. Rating
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Description CD153 is a 39-40 kD type II membrane glycoprotein, also known
as CD30 ligand or CD30L. This TNF superfamily member is expressed on
activated T cells. CD153 has been reported to stimulate B-cell proliferation,
antigen-specific antibody production, polyclonal immunoglobulin secretion,
and T cell activation and proliferation. The RM153 antibody inhibits
binding of CD153 to CD30.
TPA-stimulated (20 hrs) splenocytes stained
with purified RM153, then detected with
biotinylated anti-rat IgG followed by Sav-PE
See PE spectral data
Reactivity
Mouse
Immunogen
CHO cells transfected with mouse CD153 gene (Tnfsf8)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal
conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
Application
FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow
cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg
per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal
performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation,
immunohistochemical staining of acetone-fixed frozen sections, and in vivo blocking of CD30
binding to CD30 ligand (CD153) 1-3,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg,
Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 106408).
Application References
1. Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519.
2. Seko Y, et al. 2001. J. Pathol. 195:593.
3. Akiba H, et al. 2000. J. Exp. Med. 191:375.
4. Zhang H and GG Meadows 2005. J. Leukocyte Biol. 78:1070.PubMed
5. Zeiser R, et al. 2007. Blood 109:2225.
(PubMed link indicates
BioLegend citation)
Publication Library
RRID
AB_2207502 (BioLegend Cat. No. 106405)
Antigen Details
Structure
TNF superfamily, 39-40 kD
Distribution
Activated T cells
Function
T-B cell costimulation
Ligand Receptor
CD30
Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Horie R, et al. 1998. Semin. Immunol. 106:457.
3. Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519.
4. Shanebeck KD, et al. 1995. Eur. J. Immunol. 25:2147.
5. Bowen MA, et al. 1996. J. Immunol. 156:442.
Gene ID
21949
UniProt
View information about CD153 on UniProt.org
Documentation
Technical data sheet
Certificate of Analysis
Safety Data Sheet
Related Protocols
Cell Surface Immunofluorescence Staining Protocol
Related FAQs
There are no FAQs for this product.
Related Categories
Immunology > Mouse Immunology > Costimulatory Molecules > CD153 > RM153 >
Immunology > Mouse Immunology > CD and Related Molecules > CD153 > RM153 >
Other Formats
Description
Clone
Applications
APC anti-mouse CD153
RM153
FC
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other violations that may occur with the use of our products.
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