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Version: 1 Revision Date: 11/30/2012 PE anti-mouse CD153 Antibody Catalog# / Size 106405 / 50 µg Clone RM153 (See other available formats) Other Names CD30 ligand, CD30L, TNFSF8 Isotype Rat IgG2b, κ Ave. Rating ★★★★★ 0 reviews Description CD153 is a 39-40 kD type II membrane glycoprotein, also known as CD30 ligand or CD30L. This TNF superfamily member is expressed on activated T cells. CD153 has been reported to stimulate B-cell proliferation, antigen-specific antibody production, polyclonal immunoglobulin secretion, and T cell activation and proliferation. The RM153 antibody inhibits binding of CD153 to CD30. TPA-stimulated (20 hrs) splenocytes stained with purified RM153, then detected with biotinylated anti-rat IgG followed by Sav-PE See PE spectral data Reactivity Mouse Immunogen CHO cells transfected with mouse CD153 gene (Tnfsf8) Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody. Concentration 0.2 mg/ml Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application FC - Quality tested Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Excitation Laser Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm) Application Notes Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen sections, and in vivo blocking of CD30 binding to CD30 ligand (CD153) 1-3,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 106408). Application References 1. Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519. 2. Seko Y, et al. 2001. J. Pathol. 195:593. 3. Akiba H, et al. 2000. J. Exp. Med. 191:375. 4. Zhang H and GG Meadows 2005. J. Leukocyte Biol. 78:1070.PubMed 5. Zeiser R, et al. 2007. Blood 109:2225. (PubMed link indicates BioLegend citation) Publication Library RRID AB_2207502 (BioLegend Cat. No. 106405) Antigen Details Structure TNF superfamily, 39-40 kD Distribution Activated T cells Function T-B cell costimulation Ligand Receptor CD30 Antigen References 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Horie R, et al. 1998. Semin. Immunol. 106:457. 3. Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519. 4. Shanebeck KD, et al. 1995. Eur. J. Immunol. 25:2147. 5. Bowen MA, et al. 1996. J. Immunol. 156:442. Gene ID 21949 UniProt View information about CD153 on UniProt.org Documentation Technical data sheet Certificate of Analysis Safety Data Sheet Related Protocols Cell Surface Immunofluorescence Staining Protocol Related FAQs There are no FAQs for this product. Related Categories Immunology > Mouse Immunology > Costimulatory Molecules > CD153 > RM153 > Immunology > Mouse Immunology > CD and Related Molecules > CD153 > RM153 > Other Formats Description Clone Applications APC anti-mouse CD153 RM153 FC For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587