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Quantity Cat. No. Batch No. 100 µl 2031500201 213527 Polyclonal Antibody to Collagen Type II Host: Immunogen: Rabbit Purified human cartilage collagen Type II Presentation: Liquid, affinity purified antibody. IHC, ELISA 1)NOTOXhisto 2)Formalin Assay System: Fixation: Staining procedure: Paraffin sections: Proteolytic enzyme digestion of deparaffi-nized section before staining is necessary. For enhanced results it is recommended to use Pepsin for treatment in following working protocol: = 0,1% Pepsin in 0,5 M acetic acid for 2 hours at +37°C. After treatment slides must be well soaked with buf-fer. The antibody dilution when used the ABC-method is 1: 10 up to 1 : 50, for 1 hour at RT. Cryostat Sections: Type II Collagen Antibody can be used to label acetone fixed cryostat section or cellsmears too. The optional working dilution should be determined by the individual laboratory. Reactivity: Collagen Typ II reacts with: human Collagen Type II 100% human Collagen Type I < 1,0% Type III < 1,0% Type IV < 0,5% Type V < 0,5% human Fibronectin < 0,1% human Laminin < 0,1% Cross-Reactivity: Staining: Storage: Human, Monkey(crco pitecus), others not tested. Antibody stains cartilage and nucleus pulposees. Aliquot and store at -20°C. FOR IN VITRO USE NOT FOR DIAGNOSTIC USE Manufacturer: BIOCYC KG, Zapfholzweg 1, 14943 Luckenwalde Distributed by: Zur Anzeige wird der QuickTime™ Dekompressor „Keine“ benötigt. Schichauweg 16, 12307 Berlin, Germany Tel.: ++49 (030) 765 925-0, Fax.: ++49 (030) 765 925-55 E-Mail: [email protected] Internet: www.quartett.com