Download Purified anti-tartrate-resistant acid phosphatase (TRAcP)

Version: 2
Revision Date: 01/22/2014
Purified anti-tartrate-resistant acid phosphatase (TRAcP)
Antibody
Catalog# / Size
648402 / 100 µg
648401 / 25 µg
Clone
9C5 (See other available formats)
Other Names
Tartrate-resistant acid phosphatase, purple acid
phosphatase, acid phosphatase 5, TRACP
Isotype
Mouse IgG2b, κ
Ave. Rating
★★★★★ 0 reviews
Description
Serum tartrate-resistant acid phosphatase exists as two isoforms (TRAcP5a
and 5b). They are derived by differential-posttranslational processing of a
common gene. Serum TRAcP5a is secreted by macrophages and dendritic
cells and increased in many patients of rheumatoid arthritis. Serum
TRAcP5b is produced from osteoclasts and elevated during bone
resorption. In addition, this antibody labels hairy leukemia cells with high
degree of sensitivity and specificity.
Reactivity
Human, Cross-Reactivity: Mouse, Rat
Western blot analysis of recombinant human
TRACP/PAP/ACP5 protein (lane 1) and
PBMC (lane 2) using anti-TRACP antibody,
clone 9C5.
Immunogen
TRAcP purified from hairy cell leukemia spleen tissue
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application
WB - Quality tested
IHC, IP - Reported in the literature
Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the
suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be
titrated for optimal performance for each application.
Application Notes
Clone 9C5 reacts with both TRAcP 5a and 5b isoforms.2 Additional reported applications (for the
relevant formats) include: immunohistochemistry of formalin-fixed paraffin-embedded sections1
and immunoprecipitation 1.
Application References
1. Janckila AJ, et al. 1995. Blood 85:2839. (IHC, IP)
2. Janckila AJ, et al. 2005. J. Leukocyte Biol. 77:209.
3. Toro EJ, et al. 2012. J Biol Chem. 287:17984. PubMed
(PubMed link indicates
BioLegend citation)
Publication Library
RRID
AB_2257826 (BioLegend Cat. No. 648402)
AB_2257827 (BioLegend Cat. No. 648401)
Antigen Details
Structure
In serum, TRAcP exists as two related glycoprotein isoforms derived by differential processing of
a single gene product. Mature human TRAcP 5a isoform is a continuous polypeptide of
approximately 300 amino acids with Mw of around 33 kD. TRAcP5b contain
Distribution
TRAcP is expressed intracellularly at high levels in cells of monocytic lineage, including
osteoclasts, macrophages and dendritic cells.
Antigen References
1. Janckila AJ, et al. 1995. Blood 85:2839.
2. Janckila AJ, et al. 1996. J. of Histochem. and Cytochem. 44:235.
3. Janckila AJ, et al. 2005. J. Leukocyte Biol. 77:209.
Gene ID
54
UniProt
View information about TRAcP on UniProt.org
Documentation
Technical data sheet
Certificate of Analysis
Safety Data Sheet
Related Protocols
Western Blotting Protocol
Related FAQs
There are no FAQs for this product.
Related Categories
Immunology > Human Immunology > Monocyte/Macrophage > TRAcP >
Immunology > Mouse Immunology > Dendritic cells > TRAcP >
Immunology > Human Immunology > Dendritic cells > TRAcP >
Immunology > Mouse Immunology > Monocyte/Macrophage > TRAcP >
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