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Download BIOGRAPHICAL SKETCH Joanne I. Yeh joanneyeh Associate
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BIOGRAPHICAL SKETCH Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES. NAME POSITION TITLE Joanne I. Yeh Associate Professor of Structural Biology eRA COMMONS USER NAME joanneyeh EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE YEAR(s) FIELD OF STUDY INSTITUTION AND LOCATION (if applicable) Rochester Institute of Technology, Rochester, NY University of California, Berkeley, CA University of Washington, Seattle, WA Brown University, Providence, RI Brown University, Providence, RI University of Pittsburgh Medical School, Pittsburgh, PA B.S. Ph.D. Postdoctoral Assistant Prof Associate Prof Associate Prof 1985-1990 1991-1994 1995-1998 1999-2005 2005-2006 2005present Chemistry Chemistry; X-ray crystal. Biochemistry; X-ray cryst. Med. Science; Chemistry Med. Science; Chemistry Structural Biology A. Positions and Honors Positions Postdoctoral Fellow, University of Washington/HHMI 1995-1998 Assistant Professor of Medical Science, Brown University 1998-2005 Assistant Professor of Chemistry, Brown University 1999-2005 Associate Professor of Medical Science, Chemistry, Brown University 2005 Director, X-ray Crystallography Facility, University of Pittsburgh Medical School 2005-present Associate Professor of Structural Biology University of Pittsburgh Medical School 2005-present Honors Baldwin Scholarship 1985 Mead Corporation Scholarship 1987 RIT Departmental Inorganic Chemistry Award 1990 RIT Departmental Biochemistry Award 1990 NIH Molecular Biophysics Fellowship 1990 UC Biotechnology Research and Education Fellowship on Membrane Proteins 1993 NIH Postdoctoral Fellowship / NRSA 1996 March of Dimes Basil O’ Conner Scholar Award 2000 Federal Government Public Advisory Committee Service and Review Boards NIH reviewer, MSFA Study Section, standing member 2006-2010 Editorial Review Board, PLoS 2007 NIH reviewer, Biochemistry Study Section (ad hoc) 2003, 2004, 2005 American Chemical Society – Petroleum Research Fund 2004 Department of Defense, US Army Medical Research, Medical Research Program 2002 Reviewer, Biochemistry Journal, Acta Crystallographica, Nature Structural Biology, 1999-present Journal of Molecular Biology, PLoS, Biosensors Reviewer, Brookhaven National Synchrotron Light Source current B. Selected peer-reviewed publications (in chronological order). Moore J.M., Cooper D.A., Lurie I.S., Kram T.C., Carr S., Harper C., and Yeh J. (1987). Capillary gas chromatographic-electron capture detection of coca-leaf-related impurities in illicit cocaine: 2,4-diphenyl cyclobutane-1,3-dicarboxylic acids, 1,4-diphenylcyclobutane-2,3-dicarboxylic acids and their alkaloidal precursors, the truxillines. Journal of Chromatography 410(2), 297-318. PHS 398/2590 (Rev. 11/07) Biographical Sketch Format Page Milburn, M.V., Prive, G.G., Milligan, D.L., Scott, W.G., Yeh, J.I., Jancarik, J., Koshland, D. E., Jr., and Kim, S.H. (1991). Three-Dimensional Structures of the Ligand-Binding Domain of the Bacterial Aspartate Receptor With and Without a Ligand. Science 254, 1342-1347. Kim, S.-H., Prive, G.G., Yeh, J.I., Scott, W.G., and Milburn. M. (1992). A Model for Transmembrane Signaling in a Bacterial Chemotaxis Receptor. Cold Springs Harbor Symposia on Qualitative Biology LVII, 17-23. Yeh, J.I., Biemann, H.-P., Pandit, J., Koshland, Jr., D.E., and Kim, S.-H. (1993). The Three-Dimensional Structure of the Ligand-Binding Domain of a Wild-type Bacterial Chemotaxis Receptor. Journal of Biological Chemistry 268, 9787-9792. Scott, W.G., Milligan, D.L., Milburn, M.V., Prive, G.G., Yeh, J., Koshland, Jr., D.E., and Kim, S.-H. (1993). Refined Structures of the Ligand-Binding Domain of the Aspartate Receptor from Salmonella typhimurium. Journal of Molecular Biology 232, 555-573. Yeh, J.I., Claiborne, A., and Hol, W.G.J. (1996). Structure of the Native Cysteine-Sulfenic Acid Redox Center ofEnterococcal NADH Peroxidase Refined at 2.8 Å Resolution. Biochemistry 35(31), 9951-9957. Yeh, J.I., Biemann, H.-P., Prive, G., Pandit, J., Koshland, Jr., D.E., and Kim, S.-H. (1996). High Resolution Structures of the Ligand-Binding Domain of the Wild-type Bacterial Aspartate Receptor. Journal of Molecular Biology 262(2), 186-201. Claiborne, A., Crane, E.J., Parsonage, D., Yeh, J., Hol, W.G.J., and Vervoort, J (1997). NADH Peroxidase 13 from Enterococcus faecalis: Crystal Structure, C NMR Analysis, and Mechanism. In Flavins and Flavoproteins. editors K.J. Stevenson, V. Massey, and C.H. Williams, Jr., University of Calgary Press, Calgary, Alberta, Canada. Yeh, J.I., Claiborne, A., and Hol, W.G.J (1997). Structure of the Native Cysteine-Sulfenic Acid Redox Center of Enterococcal NADH Peroxidase. In Flavins and Flavoproteins. editors K.J. Stevenson, V. Massey, and C.H. Williams, Jr., University of Calgary Press, Calgary, Alberta, Canada. Yeh, J.I. and Hol, W.G.J. (1998). A Flash-Annealing Technique to Improve Diffraction Limits and Lower Mosaicity in Crystals of Glycerol Kinase. Acta Crystallographica D54, 479-480. Beale, S.I. and Yeh, J.I. (1999). Deconstructing Heme. Nature Structural Biology 6, 903-905. Claiborne, A., Yeh, J.I., Mallett,T.C., Luba, J., Crane, E.J., Charrier, V., and Parsonage, D. (1999). ProteinSulfenic Acids: Diverse Roles for an Unlikely Player in Enzyme Catalysis and Redox Regulation. Biochemistry 38, 15407-15416. Crane, E.J., Yeh, J.I., Luba, J., and Claiborne, A. (2000). Analysis of the Kinetic and Redox Properties of the NADH Peroxidase R303M Mutant: Correlation with the Crystal Structure. Biochemistry 39, 10353-10364. Claiborne, A., Mallett, C., Yeh, J.I., Luba, J., and Parsonage, D. (2001). Structural, Redox, and Mechanistic Parameters for Cysteine-Sulfenic Acid Function in Catalysis and Regulation. J. Klinman, eds. Adv. Prot. Chem 58, 215-276. Yeh, J.I. and Claiborne, A. (2002). Crystal structure of oxidized and reduced forms of NADH Peroxidase. C. Sen and L. Packer, eds. Redox Cell Biology and Genetics. Methods in Enzymology 353, 44-45. Doniger, S., Hoffman, T., and Yeh, J.I. (2002). Predicting CNS Permeability of Drug Molecules: Comparison of Neural Network and Support Vector Machine Algorithms. Journal of Computational Biology 9(6), 849-864. Yeh, J.I., Du, S., Pohl, E., and Cane, D.E. (2002). Multistate Binding in Pyridoxine 5-‘Phosphate Synthase: 1.96 Å Crystal Structure in Complex with 1-Deoxy-D-xylulose Phosphate. Biochemistry 41, 11649-11657. PHS 398/2590 (Rev. 11/07) Biographical Sketch Format Page Yeh, J.I. (2003). A Manual Nanoscale Method for Protein Crystallization. Acta Cryst D. 59(8), 1408-1413. Yeh, J.I., Charrier, V., Paulo, J., Hou, L., Darbon, E., Claiborne, A., Hol, W.G.J., and Deutscher, J. (2004). Structures of Enterococcal Glycerol Kinase in the Absence and Presence of Glycerol: correlation of conformation to substrate binding and a mechanism of activation by phosphorylation. Biochemistry 43(2); 362373 Vahedi-Faridi, A., Stojanoff, V., and Yeh, J.I. (2005). The effects of flash-annealing on glycerol kinase crystals. Acta Cryst D61, 982-989. Yeh, J.I., Zimmt, M., and Zimmerman, A. (2005). Nanowiring of a Redox Enzyme by Metallized Peptides. Biosensors and Bioelectronics 21(6), 973-978. Yeh, J.I., Du, S., Tortajada, A., Paulo, J., and Zhang, S. (2005). Peptergents: Peptide Detergents that Improve Stability and Functionality of an Integral Membrane Protein, Glycerol-3-Phosphate Dehydrogenase. Biochemistry 44 (51), 16912 - 16919. Mao, N. and Yeh, J.I. (2005). Membrane Protein Prediction of M.tb using SVM Algorithm. Special issue on GENSIPS, IEEE Trans. on Signal Transduction. Withey, G.D., Lazarek, A.D., Tzolov, M.B., Yin., A., Aich, P., Yeh, J.I., and Xu, J. (2005). Ultra-high redox enzyme signal transduction using highly ordered carbon nanotube array electrodes. Biosensors and Bioelectronics, in press (online Aug80 ust 29, 2005). Yeh, J.I. and Mao, N. (2006). Prediction of Membrane Proteins in Mycobacterium tuberculosis using Support Vector Machine Algorithm. Journal of Computational Biology 13(1), 128-131. Yeh, J.I. (2006). Coordinated Biosensors: integrated systems for ultra sensitive detection. In Nanotechnologies for Life Sciences vol 7. Challa Kumar, Editor, Wiley-VCH, in press. Xia, T., Kovochich, M., Brant, J., Hotze, M., Sempf, J., Oberley, T., Sioutas, C., Yeh, J.I., Wiesner, M.R., and Nel, A.E. (2006). Comparison of the Abilities of Ambient and Manufactured Nanoparticles to Induce Cellular Toxicity According to an Oxidative Stress Paradigm. Nanoletters 6, 1794-1807. Yeh, J.I., Lazarek, A., Kim, J-H., Xu, J., and Du, S. (2007). Peptide Nanowires for Coordination of Peroxidase Biosensors to Carbon Nanotube Electrode Arrays, Biosensors and Bioelectronics 23(4), 568-574. Yeh, J.I.. and Beale, S. (2007). Calorimetric Approaches to Characterizing Effects of Additives on Protein Crystallization. Crystal Growth and Design (ACS) 7(11), 2134-2139. Yeh, J.I. (2007). Additives and microcalorimetric approaches for optimization of crystallization. In Protein Crystallization: Techniques, Strategies, and Tips. Terese Bergfors, Editor, 2nd edition. Shi, H. and Yeh, J.I. (2007). I. Recent Developments in Nanoelectrode for Biological Measurements. Nanomedicine 2(5), 587-598. Shi, H., Xia, T., Nel, A.E. and Yeh, J.I. (2007). II. Coordinated Biosensors and Applications of Nanobiosensors for Biological and Medical applications. Nanomedicine 2(5), 599-614. Kovochich, M., Xia, T., Xu, J., Yeh, J.I., and Nel, A.E. (2007). Principles and Methods for the assessment of nanomaterial toxicity. In Toxicological Impact of Materials. M. Weisner, Editor. PHS 398/2590 (Rev. 11/07) Biographical Sketch Format Page Yeh, J.I., Du, S., Xia, T., Lazareck, A., Kim, J-H., Xu, J., and Nel, A.E. (2007). Coordinated Nanobiosensors for Enhanced Detection: Integration of Three-Dimensional Structures to Toxicological Applications. ECS Transactions 3(29), 115-126. Yeh, J.I., Chinte, U., and Du, S. (2008). Structure of glycerol-3-phosphate dehydrogenase, an essential monotopic membrane enzyme involved in respiration and metabolism. PNAS USA 9 (online Feb. 22,2008). C. Research Support NIH R01 GM/AI66466: PI J.I. Yeh 07/01/02-06/01/08 Structural Studies on Metabolic Membrane Proteins Major focus of this study is on the crystal structure determination of membrane proteins involved in glycerol and oxidative metabolism in Gram-positive, pathogenic bacteria. DARPA / AFSOR MURI:co-PI J.I. Yeh 06/01/03-05/01/08 Biological Interface for Ultrasensitive Detection and Signal Transduction Major goals of this multi-institutional study involve the development of methodologies to link biological macromolecules to build highly sensitive and specific nanosensors for medical and other applications. This study includes directed chemical synthesis, protein and DNA engineering, new materials development, and microfluidics design for the delivery of array sensor systems. Co-PIs: J. Xu, R. Beresford (Brown), S. Zhang (MIT), I. Chaiken (Drexel), C. Martin (UFL), S. Kelley (Boston C.), and N. Lebedev (NRL). NIH P50: PI: A. Gronenborn; X-ray Director J.I. Yeh 10/01/07-09/30/12 University of Pittsburgh Center for HIV Protein Interactions (PCHPI) (Crystallography Core, Director) Completed: NSF NIRT Co-PIs: S. Ling, J.I. Yeh, A. Meller, D. Nelson 05/01/04-08/30/05 DNA Sequencing and Translocation Studies Using Electrically-Addressable Nanopore Arrays Role: co-PI March of Dimes FY2001BOC-62 J.I.Yeh 02/01/01-01/31/04 Glycerol-3-Phosphate Dehydrogenase: a new molecular target in Pseudomonas aeruginosa Major goals of this study are to identify and characterize a new molecular target in P. aeruginosa and to develop inhibitors based on these structural results. No overlap with the proposal being submitted. Role: PI DARPA / AFOSR J.I. Yeh 05/01/02-01/01/04 Engineering DNA for Interfacing Redox with Read-Out Major goal of this feasibility study is to provide the foundation for utilizing biological macromolecules as sensors / detectors for integrating with electronic circuitry in microfluidic arrays. Co-PI: J. Xu (Brown) No overlap with the proposal being submitted. Role: PI Soloman Grant J.I. Yeh 01/01/2000 Structural studies on proteins mediating key metabolic pathways in pathogenic bacteria. P.I. J.I.Yeh Rhode Island Foundation J.I. Yeh 06/01/1999 PI J.I.Yeh Equipment Grants: MF-29305 01/01/99 Markey Foundation Acquisition of Diffraction System for Structural Biology and Biochemistry NSF-0116441 D. Cane 09/01/01 NSF Major Research Instrumentation Acquisition of a High Resolution Mass Spectrometer Role: co-PI (J. Yeh, J. Martin, C. Seto, A. Basu) PHS 398/2590 (Rev. 11/07) Biographical Sketch Format Page