procite - UWI St. Augustine
... many different proteins. The substrate specificity [1] of this enzyme can be summarized as follows: (1) Under comparable conditions Ser is favored over Thr. (2) An acidic residue (either Asp or Glu) must be present three residues from the C-terminal of the phosphate acceptor site. (3) Additional aci ...
... many different proteins. The substrate specificity [1] of this enzyme can be summarized as follows: (1) Under comparable conditions Ser is favored over Thr. (2) An acidic residue (either Asp or Glu) must be present three residues from the C-terminal of the phosphate acceptor site. (3) Additional aci ...
Midazolam Oxidation by Cytochrome P450 3A4 and Active
... CYP3A4 (Gorski et al., 1994; Kuehl et al., 2001). The oxidation of MDZ by CYP3A4 has been the focus of many in vitro investigations (Kronbach et al., 1989; Gorski et al., 1994; Ghosal et al., 1996; Maenpaa et al., 1998; Hosea et al., 2000; Wang et al., 2000). These studies have found very different ...
... CYP3A4 (Gorski et al., 1994; Kuehl et al., 2001). The oxidation of MDZ by CYP3A4 has been the focus of many in vitro investigations (Kronbach et al., 1989; Gorski et al., 1994; Ghosal et al., 1996; Maenpaa et al., 1998; Hosea et al., 2000; Wang et al., 2000). These studies have found very different ...
npr review - Olivamine
... has two hydrophobic phenyl domains that are connected by a flexible linker (Fig. 1), and molecular docking studies have found that curcumin can adopt many different conformations suitable for maximizing hydrophobic contacts with the protein to which it is bound. For example, the phenyl rings of curc ...
... has two hydrophobic phenyl domains that are connected by a flexible linker (Fig. 1), and molecular docking studies have found that curcumin can adopt many different conformations suitable for maximizing hydrophobic contacts with the protein to which it is bound. For example, the phenyl rings of curc ...
Pyruvate dehydrogenase - Wageningen UR E
... complexes. For a better understanding however, the most important features of the other components of the complex will be discussed in this section [for reviews see Perham, 1991; Mattevi etal., 1992a;Berg&deKok, 1997;deKok etal., 1998;Perham,2001]. Limited proteolysis studies revealed that E2 has a ...
... complexes. For a better understanding however, the most important features of the other components of the complex will be discussed in this section [for reviews see Perham, 1991; Mattevi etal., 1992a;Berg&deKok, 1997;deKok etal., 1998;Perham,2001]. Limited proteolysis studies revealed that E2 has a ...
Structure and Function of Thymosin β4
... In order to determine the structure and binding motif of the actin binding site in thymosin β4, mutational analysis was performed. The binding motif observed in virtually all of the β thymosins is 17LKKTET22 and, furthermore, the first three residues are also found in many other actin binding protei ...
... In order to determine the structure and binding motif of the actin binding site in thymosin β4, mutational analysis was performed. The binding motif observed in virtually all of the β thymosins is 17LKKTET22 and, furthermore, the first three residues are also found in many other actin binding protei ...
Exploring the directionality of Escherichia coli formate
... a formate-specific channel, FocA, in order to achieve this as efficiently as possible (Doberenz et al. 2014). However, under fermentative conditions, the secreted formate cannot be further respired and instead accumulates in the growth medium. This, together with a drop in the external pH caused by ...
... a formate-specific channel, FocA, in order to achieve this as efficiently as possible (Doberenz et al. 2014). However, under fermentative conditions, the secreted formate cannot be further respired and instead accumulates in the growth medium. This, together with a drop in the external pH caused by ...
Microsoft Word
... with TPO is also inhibited by human Fab TR1.9 [38]. It was demonstrated that P715 and D717 were directly involved in binding of this human antibody [24]. The third candidate key contact point for TR1.9 binding was K713 [28]. Further studies showed that although K713 is a contact residue for TR1.9 it ...
... with TPO is also inhibited by human Fab TR1.9 [38]. It was demonstrated that P715 and D717 were directly involved in binding of this human antibody [24]. The third candidate key contact point for TR1.9 binding was K713 [28]. Further studies showed that although K713 is a contact residue for TR1.9 it ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... A novel compound, the fatty acid synthase (FAS) inhibitor C75, has been proposed to pharmacologically regulate CPT I activity (Price, 2001). Structurally, C75 is a cell-permeable α-methylene-γ-butyrolactone designed to be less reactive and potentially safer than cerulenin, a natural product obtained ...
... A novel compound, the fatty acid synthase (FAS) inhibitor C75, has been proposed to pharmacologically regulate CPT I activity (Price, 2001). Structurally, C75 is a cell-permeable α-methylene-γ-butyrolactone designed to be less reactive and potentially safer than cerulenin, a natural product obtained ...
Structure of a Pheromone Receptor-Associated MHC Molecule with
... thermally unstable, suggesting that its groove is normally occupied. However, M10.5 does not bind endogenous peptides when expressed in mammalian cells or when offered a mixture of class I–binding peptides. The F pocket side of the M10.5 groove is open, suggesting that ligands larger than 8–10-mer c ...
... thermally unstable, suggesting that its groove is normally occupied. However, M10.5 does not bind endogenous peptides when expressed in mammalian cells or when offered a mixture of class I–binding peptides. The F pocket side of the M10.5 groove is open, suggesting that ligands larger than 8–10-mer c ...
The enzymes of biotin dependent CO2 metabolism: What structures
... in the active site cleft of the enzyme. As can be seen, the subunit is somewhat asymmetric with a small domain extending away from the main body of the molecule. Overall the architecture of the enzyme can be envisioned in terms of three domains. The A-domain consists of a five-stranded parallel b-sh ...
... in the active site cleft of the enzyme. As can be seen, the subunit is somewhat asymmetric with a small domain extending away from the main body of the molecule. Overall the architecture of the enzyme can be envisioned in terms of three domains. The A-domain consists of a five-stranded parallel b-sh ...
Biological ontologies for human functional annotation and
... “The GENIA ontology is intended to be a formal model of cell signaling reactions in human. ... use of the GENIA ontology is to provide a basis for integrated view of multiple databases“ ...
... “The GENIA ontology is intended to be a formal model of cell signaling reactions in human. ... use of the GENIA ontology is to provide a basis for integrated view of multiple databases“ ...
Pollen Exine Pattern Formation is Dependent on Three Major
... exine has been widely carried out, and has clarified a possible component of exine, which is termed sporopollenin. Sporopollenin is used as a generic term for the exine constituent. Several lines of biochemical evidence clearly indicate that sporopollenin consists of phenylpropanoids, phenolics and ...
... exine has been widely carried out, and has clarified a possible component of exine, which is termed sporopollenin. Sporopollenin is used as a generic term for the exine constituent. Several lines of biochemical evidence clearly indicate that sporopollenin consists of phenylpropanoids, phenolics and ...
Cytotoxic heteroleptic heptacoordinate salan
... is quite uncommon for group IV complexes and besides two N,N 0 -bis(salicylidene)alkylenediamine (salen) complexes,18 only two bis(salan) complexes are known.19 [(L1)2Zr] differs from these by its methyl-substitution at the nitrogen; while the former are originating from secondary amines (salanH4), t ...
... is quite uncommon for group IV complexes and besides two N,N 0 -bis(salicylidene)alkylenediamine (salen) complexes,18 only two bis(salan) complexes are known.19 [(L1)2Zr] differs from these by its methyl-substitution at the nitrogen; while the former are originating from secondary amines (salanH4), t ...
The C-terminal domain of the Rhizobium leguminosarum
... in the presence of hesperetin. Derivatives of pIJ1089 and pIJ1887 containing TnphoA were transferred to R. leguminosarum strain 8401 by conjugation. NodC–PhoA fusions expressing high levels of alkaline phosphatase could be identified in R. leguminosarum on plates containing hesperetin to induce nod ...
... in the presence of hesperetin. Derivatives of pIJ1089 and pIJ1887 containing TnphoA were transferred to R. leguminosarum strain 8401 by conjugation. NodC–PhoA fusions expressing high levels of alkaline phosphatase could be identified in R. leguminosarum on plates containing hesperetin to induce nod ...
Unique amino acid signatures that are evolutionarily conserved
... and the intermediate filaments (IFs) (Ku et al., 1999; Alberts et al., 2008). IFs are encoded by more than 70 unique genes, which are typically specifically expressed in unique cell types, whereas microfilaments and microtubules are far more widespread in their expression profiles (Moll et al., 1982 ...
... and the intermediate filaments (IFs) (Ku et al., 1999; Alberts et al., 2008). IFs are encoded by more than 70 unique genes, which are typically specifically expressed in unique cell types, whereas microfilaments and microtubules are far more widespread in their expression profiles (Moll et al., 1982 ...
Cloning of adiponectin receptors that mediate antidiabetic metabolic
... cyclic AMP, cyclic GMP and intracellular calcium levels (Supplementary Fig. 2c, d). In contrast, expression of AdipoR1 enhanced increases in PPAR-a ligand activity by both globular and full-length adiponectin (Supplementary Fig. 2e). Expression of AdipoR1 or AdipoR2 in C2C12 myocytes (Supplementary ...
... cyclic AMP, cyclic GMP and intracellular calcium levels (Supplementary Fig. 2c, d). In contrast, expression of AdipoR1 enhanced increases in PPAR-a ligand activity by both globular and full-length adiponectin (Supplementary Fig. 2e). Expression of AdipoR1 or AdipoR2 in C2C12 myocytes (Supplementary ...
Structure of the Actin-Myosin Complex and Its Implications for
... surface feature identified as the NH2-terminal domain of skeletal muscle S1 (20). Attempts to rotate the head to align the 13 barrel and the surface feature result in a misfit for the rest of the molecule. In that this barrel projects away from the rest of the molecule it may adopt different positio ...
... surface feature identified as the NH2-terminal domain of skeletal muscle S1 (20). Attempts to rotate the head to align the 13 barrel and the surface feature result in a misfit for the rest of the molecule. In that this barrel projects away from the rest of the molecule it may adopt different positio ...
Mycotoxin
... the diet to types of fat that can be stored and used by the body. Triglycerides are one of the forms of fat stored by the body and used for energy and new cell formation. The break down of fats in the liver can be disrupted by alcoholism, malnutrition, pregnancy, or poisoning. In fatty liver, large ...
... the diet to types of fat that can be stored and used by the body. Triglycerides are one of the forms of fat stored by the body and used for energy and new cell formation. The break down of fats in the liver can be disrupted by alcoholism, malnutrition, pregnancy, or poisoning. In fatty liver, large ...
Structural and Functional Studies on Proteinaceous Metallocarboxypeptidase Inhibitors Joan López Arolas
... Peptidase inhibitors Peptidase inhibitors are important tools of nature for regulating the proteolytic activity of their target proteases, and for blocking them in emergency cases (Bode & Huber, 1992). All naturally occurring inhibitors directed toward endogenous peptidases, i.e. against proteolytic ...
... Peptidase inhibitors Peptidase inhibitors are important tools of nature for regulating the proteolytic activity of their target proteases, and for blocking them in emergency cases (Bode & Huber, 1992). All naturally occurring inhibitors directed toward endogenous peptidases, i.e. against proteolytic ...
Cyclic-‐di-‐AMP: another second messenger enters the fray
... remains unknown, analysis of associated domains or the operon structure in which DACs are encoded can provide insights into the role of these proteins. The most abundant domain architecture for ...
... remains unknown, analysis of associated domains or the operon structure in which DACs are encoded can provide insights into the role of these proteins. The most abundant domain architecture for ...
topological changes in the cyp3a4 active site probed with
... plays a significant role in the metabolism of a wide variety of drugs (Guengerich, 1999; Nebert and Russell, 2002). Because of its pharmacological significance and the potential for drug-drug interactions, CYP3A4 has been the subject of a large number of studies focused on elucidating the relationsh ...
... plays a significant role in the metabolism of a wide variety of drugs (Guengerich, 1999; Nebert and Russell, 2002). Because of its pharmacological significance and the potential for drug-drug interactions, CYP3A4 has been the subject of a large number of studies focused on elucidating the relationsh ...
Engineering subunit association of multisubunit proteins
... barrel surfaces have complementary curvatures, making numerous intersubunit van der Waals and electrostatic interactions. The tetramer is formed by the association of two such stable dimers, which are connected relatively weakly across a small intersubunit contact area. The difference in calculated ...
... barrel surfaces have complementary curvatures, making numerous intersubunit van der Waals and electrostatic interactions. The tetramer is formed by the association of two such stable dimers, which are connected relatively weakly across a small intersubunit contact area. The difference in calculated ...
Detection of marine toxins using cell-based assays and
... with toxins and alter ecosystems [7]. These events are referred to “Harmful Algal Blooms (HABs)”. HAB species involve a wide range of organisms: dinoflagellates, flagellates, cyanobacteria, diatoms and others. It is worth noting that among these different groups, by far the group of dinoflagellates ...
... with toxins and alter ecosystems [7]. These events are referred to “Harmful Algal Blooms (HABs)”. HAB species involve a wide range of organisms: dinoflagellates, flagellates, cyanobacteria, diatoms and others. It is worth noting that among these different groups, by far the group of dinoflagellates ...
Case Study: BPTI
... pancreas. BPTI is a member of the serine protease family of inhibitors [1]. A hallmark of this class of enzymes are the many conserved cysteine residues that form disulfide bonds stabilizing the proteins’ three-dimensional structures. BPTI has a relatively broad specificity in that it can inhibit se ...
... pancreas. BPTI is a member of the serine protease family of inhibitors [1]. A hallmark of this class of enzymes are the many conserved cysteine residues that form disulfide bonds stabilizing the proteins’ three-dimensional structures. BPTI has a relatively broad specificity in that it can inhibit se ...
Anthrax toxin
Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis—the causative agent of anthrax. The toxin was first discovered by Harry Smith in 1954. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). These three protein components act together to impart their physiological effects. Assembled complexes containing the toxin components are endocytosed. In the endosome, the enzymatic components of the toxin translocate into the cytoplasm of a target cell. Once in the cytosol, the enzymatic components of the toxin disrupts various immune cell functions, namely cellular signaling and cell migration. The toxin may even induce cell lysis, as is observed for macrophage cells. Anthrax toxin allows the bacteria to evade the immune system, proliferate, and ultimately kill the host animal. Research on anthrax toxin also provides insight into the generation of macromolecular assemblies, and on protein translocation, pore formation, endocytosis, and other biochemical processes.