Download BIOGRAPHICAL SKETCH Joanne I. Yeh joanneyeh Associate

BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.
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NAME
POSITION TITLE
Joanne I. Yeh
Associate Professor of Structural Biology
eRA COMMONS USER NAME
joanneyeh
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
DEGREE
YEAR(s)
FIELD OF STUDY
INSTITUTION AND LOCATION
(if applicable)
Rochester Institute of Technology, Rochester, NY
University of California, Berkeley, CA
University of Washington, Seattle, WA
Brown University, Providence, RI
Brown University, Providence, RI
University of Pittsburgh Medical School, Pittsburgh, PA
B.S.
Ph.D.
Postdoctoral
Assistant
Prof
Associate
Prof
Associate
Prof
1985-1990
1991-1994
1995-1998
1999-2005
2005-2006
2005present
Chemistry
Chemistry; X-ray crystal.
Biochemistry; X-ray cryst.
Med. Science; Chemistry
Med. Science; Chemistry
Structural Biology
A. Positions and Honors
Positions
Postdoctoral Fellow, University of Washington/HHMI 1995-1998
Assistant Professor of Medical Science, Brown University 1998-2005
Assistant Professor of Chemistry, Brown University 1999-2005
Associate Professor of Medical Science, Chemistry, Brown University 2005
Director, X-ray Crystallography Facility, University of Pittsburgh Medical School 2005-present
Associate Professor of Structural Biology University of Pittsburgh Medical School 2005-present
Honors
Baldwin Scholarship 1985
Mead Corporation Scholarship 1987
RIT Departmental Inorganic Chemistry Award 1990
RIT Departmental Biochemistry Award 1990
NIH Molecular Biophysics Fellowship 1990
UC Biotechnology Research and Education Fellowship on Membrane Proteins 1993
NIH Postdoctoral Fellowship / NRSA 1996
March of Dimes Basil O’ Conner Scholar Award 2000
Federal Government Public Advisory Committee Service and Review Boards
NIH reviewer, MSFA Study Section, standing member 2006-2010
Editorial Review Board, PLoS 2007
NIH reviewer, Biochemistry Study Section (ad hoc) 2003, 2004, 2005
American Chemical Society – Petroleum Research Fund 2004
Department of Defense, US Army Medical Research, Medical Research Program 2002
Reviewer, Biochemistry Journal, Acta Crystallographica, Nature Structural Biology, 1999-present
Journal of Molecular Biology, PLoS, Biosensors
Reviewer, Brookhaven National Synchrotron Light Source current
B. Selected peer-reviewed publications (in chronological order).
Moore J.M., Cooper D.A., Lurie I.S., Kram T.C., Carr S., Harper C., and Yeh J. (1987). Capillary gas
chromatographic-electron capture detection of coca-leaf-related impurities in illicit cocaine: 2,4-diphenyl
cyclobutane-1,3-dicarboxylic acids, 1,4-diphenylcyclobutane-2,3-dicarboxylic acids and their alkaloidal
precursors, the truxillines. Journal of Chromatography 410(2), 297-318.
PHS 398/2590 (Rev. 11/07)
Biographical Sketch Format Page
Milburn, M.V., Prive, G.G., Milligan, D.L., Scott, W.G., Yeh, J.I., Jancarik, J., Koshland, D. E., Jr., and Kim, S.H. (1991). Three-Dimensional Structures of the Ligand-Binding Domain of the Bacterial Aspartate Receptor
With and Without a Ligand. Science 254, 1342-1347.
Kim, S.-H., Prive, G.G., Yeh, J.I., Scott, W.G., and Milburn. M. (1992). A Model for Transmembrane Signaling
in a Bacterial Chemotaxis Receptor. Cold Springs Harbor Symposia on Qualitative Biology LVII, 17-23.
Yeh, J.I., Biemann, H.-P., Pandit, J., Koshland, Jr., D.E., and Kim, S.-H. (1993). The Three-Dimensional
Structure of the Ligand-Binding Domain of a Wild-type Bacterial Chemotaxis Receptor. Journal of Biological
Chemistry 268, 9787-9792.
Scott, W.G., Milligan, D.L., Milburn, M.V., Prive, G.G., Yeh, J., Koshland, Jr., D.E., and Kim, S.-H. (1993).
Refined Structures of the Ligand-Binding Domain of the Aspartate Receptor from Salmonella typhimurium.
Journal of Molecular Biology 232, 555-573.
Yeh, J.I., Claiborne, A., and Hol, W.G.J. (1996). Structure of the Native Cysteine-Sulfenic Acid Redox Center
ofEnterococcal NADH Peroxidase Refined at 2.8 Å Resolution. Biochemistry 35(31), 9951-9957.
Yeh, J.I., Biemann, H.-P., Prive, G., Pandit, J., Koshland, Jr., D.E., and Kim, S.-H. (1996). High Resolution
Structures of the Ligand-Binding Domain of the Wild-type Bacterial Aspartate Receptor. Journal of Molecular
Biology 262(2), 186-201.
Claiborne, A., Crane, E.J., Parsonage, D., Yeh, J., Hol, W.G.J., and Vervoort, J (1997). NADH Peroxidase
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from Enterococcus faecalis: Crystal Structure, C NMR Analysis, and Mechanism. In Flavins and
Flavoproteins. editors K.J. Stevenson, V. Massey, and C.H. Williams, Jr., University of Calgary Press,
Calgary, Alberta, Canada.
Yeh, J.I., Claiborne, A., and Hol, W.G.J (1997). Structure of the Native Cysteine-Sulfenic Acid Redox Center
of Enterococcal NADH Peroxidase. In Flavins and Flavoproteins. editors K.J. Stevenson, V. Massey, and
C.H. Williams, Jr., University of Calgary Press, Calgary, Alberta, Canada.
Yeh, J.I. and Hol, W.G.J. (1998). A Flash-Annealing Technique to Improve Diffraction Limits and Lower
Mosaicity in Crystals of Glycerol Kinase. Acta Crystallographica D54, 479-480.
Beale, S.I. and Yeh, J.I. (1999). Deconstructing Heme. Nature Structural Biology 6, 903-905.
Claiborne, A., Yeh, J.I., Mallett,T.C., Luba, J., Crane, E.J., Charrier, V., and Parsonage, D. (1999). ProteinSulfenic Acids: Diverse Roles for an Unlikely Player in Enzyme Catalysis and Redox Regulation. Biochemistry
38, 15407-15416.
Crane, E.J., Yeh, J.I., Luba, J., and Claiborne, A. (2000). Analysis of the Kinetic and Redox Properties of the
NADH Peroxidase R303M Mutant: Correlation with the Crystal Structure. Biochemistry 39, 10353-10364.
Claiborne, A., Mallett, C., Yeh, J.I., Luba, J., and Parsonage, D. (2001). Structural, Redox, and Mechanistic
Parameters for Cysteine-Sulfenic Acid Function in Catalysis and Regulation. J. Klinman, eds. Adv. Prot. Chem
58, 215-276.
Yeh, J.I. and Claiborne, A. (2002). Crystal structure of oxidized and reduced forms of NADH Peroxidase. C.
Sen and L. Packer, eds. Redox Cell Biology and Genetics. Methods in Enzymology 353, 44-45.
Doniger, S., Hoffman, T., and Yeh, J.I. (2002). Predicting CNS Permeability of Drug Molecules: Comparison of
Neural Network and Support Vector Machine Algorithms. Journal of Computational Biology 9(6), 849-864.
Yeh, J.I., Du, S., Pohl, E., and Cane, D.E. (2002). Multistate Binding in Pyridoxine 5-‘Phosphate Synthase:
1.96 Å Crystal Structure in Complex with 1-Deoxy-D-xylulose Phosphate. Biochemistry 41, 11649-11657.
PHS 398/2590 (Rev. 11/07)
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Yeh, J.I. (2003). A Manual Nanoscale Method for Protein Crystallization. Acta Cryst D. 59(8), 1408-1413.
Yeh, J.I., Charrier, V., Paulo, J., Hou, L., Darbon, E., Claiborne, A., Hol, W.G.J., and Deutscher, J. (2004).
Structures of Enterococcal Glycerol Kinase in the Absence and Presence of Glycerol: correlation of
conformation to substrate binding and a mechanism of activation by phosphorylation. Biochemistry 43(2); 362373
Vahedi-Faridi, A., Stojanoff, V., and Yeh, J.I. (2005). The effects of flash-annealing on glycerol kinase
crystals. Acta Cryst D61, 982-989.
Yeh, J.I., Zimmt, M., and Zimmerman, A. (2005). Nanowiring of a Redox Enzyme by Metallized Peptides.
Biosensors and Bioelectronics 21(6), 973-978.
Yeh, J.I., Du, S., Tortajada, A., Paulo, J., and Zhang, S. (2005). Peptergents: Peptide Detergents that
Improve Stability and Functionality of an Integral Membrane Protein, Glycerol-3-Phosphate Dehydrogenase.
Biochemistry 44 (51), 16912 - 16919.
Mao, N. and Yeh, J.I. (2005). Membrane Protein Prediction of M.tb using SVM Algorithm. Special issue on
GENSIPS, IEEE Trans. on Signal Transduction.
Withey, G.D., Lazarek, A.D., Tzolov, M.B., Yin., A., Aich, P., Yeh, J.I., and Xu, J. (2005). Ultra-high redox
enzyme signal transduction using highly ordered carbon nanotube array electrodes. Biosensors and
Bioelectronics, in press (online Aug80
ust 29, 2005).
Yeh, J.I. and Mao, N. (2006). Prediction of Membrane Proteins in Mycobacterium tuberculosis using Support
Vector Machine Algorithm. Journal of Computational Biology 13(1), 128-131.
Yeh, J.I. (2006). Coordinated Biosensors: integrated systems for ultra sensitive detection. In
Nanotechnologies for Life Sciences vol 7. Challa Kumar, Editor, Wiley-VCH, in press.
Xia, T., Kovochich, M., Brant, J., Hotze, M., Sempf, J., Oberley, T., Sioutas, C., Yeh, J.I., Wiesner, M.R.,
and Nel, A.E. (2006). Comparison of the Abilities of Ambient and Manufactured Nanoparticles to Induce
Cellular Toxicity According to an Oxidative Stress Paradigm. Nanoletters 6, 1794-1807.
Yeh, J.I., Lazarek, A., Kim, J-H., Xu, J., and Du, S. (2007). Peptide Nanowires for Coordination of Peroxidase
Biosensors to Carbon Nanotube Electrode Arrays, Biosensors and Bioelectronics 23(4), 568-574.
Yeh, J.I.. and Beale, S. (2007). Calorimetric Approaches to Characterizing Effects of Additives on Protein
Crystallization. Crystal Growth and Design (ACS) 7(11), 2134-2139.
Yeh, J.I. (2007). Additives and microcalorimetric approaches for optimization of crystallization. In Protein
Crystallization: Techniques, Strategies, and Tips. Terese Bergfors, Editor, 2nd edition.
Shi, H. and Yeh, J.I. (2007). I. Recent Developments in Nanoelectrode for Biological Measurements.
Nanomedicine 2(5), 587-598.
Shi, H., Xia, T., Nel, A.E. and Yeh, J.I. (2007). II. Coordinated Biosensors and Applications of
Nanobiosensors for Biological and Medical applications. Nanomedicine 2(5), 599-614.
Kovochich, M., Xia, T., Xu, J., Yeh, J.I., and Nel, A.E. (2007). Principles and Methods for the assessment
of nanomaterial toxicity. In Toxicological Impact of Materials. M. Weisner, Editor.
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Yeh, J.I., Du, S., Xia, T., Lazareck, A., Kim, J-H., Xu, J., and Nel, A.E. (2007). Coordinated Nanobiosensors
for Enhanced Detection: Integration of Three-Dimensional Structures to Toxicological Applications. ECS
Transactions 3(29), 115-126.
Yeh, J.I., Chinte, U., and Du, S. (2008). Structure of glycerol-3-phosphate dehydrogenase, an
essential monotopic membrane enzyme involved in respiration and metabolism. PNAS USA 9
(online Feb. 22,2008).
C. Research Support
NIH R01 GM/AI66466: PI J.I. Yeh 07/01/02-06/01/08
Structural Studies on Metabolic Membrane Proteins
Major focus of this study is on the crystal structure determination of membrane proteins involved in glycerol
and oxidative metabolism in Gram-positive, pathogenic bacteria.
DARPA / AFSOR MURI:co-PI J.I. Yeh 06/01/03-05/01/08
Biological Interface for Ultrasensitive Detection and Signal Transduction
Major goals of this multi-institutional study involve the development of methodologies to link biological
macromolecules to build highly sensitive and specific nanosensors for medical and other applications. This
study includes directed chemical synthesis, protein and DNA engineering, new materials development, and
microfluidics design for the delivery of array sensor systems. Co-PIs: J. Xu, R. Beresford (Brown), S. Zhang
(MIT), I. Chaiken (Drexel), C. Martin (UFL), S. Kelley (Boston C.), and N. Lebedev (NRL).
NIH P50: PI: A. Gronenborn; X-ray Director J.I. Yeh 10/01/07-09/30/12 University of Pittsburgh Center for
HIV Protein Interactions (PCHPI) (Crystallography Core, Director)
Completed:
NSF NIRT Co-PIs: S. Ling, J.I. Yeh, A. Meller, D. Nelson 05/01/04-08/30/05 DNA Sequencing and
Translocation Studies Using Electrically-Addressable Nanopore Arrays Role: co-PI
March of Dimes FY2001BOC-62 J.I.Yeh 02/01/01-01/31/04
Glycerol-3-Phosphate Dehydrogenase: a new molecular target in Pseudomonas aeruginosa Major goals of this
study are to identify and characterize a new molecular target in P. aeruginosa and to develop inhibitors based
on these structural results. No overlap with the proposal being submitted. Role: PI
DARPA / AFOSR J.I. Yeh 05/01/02-01/01/04
Engineering DNA for Interfacing Redox with Read-Out
Major goal of this feasibility study is to provide the foundation for utilizing biological macromolecules as
sensors / detectors for integrating with electronic circuitry in microfluidic arrays. Co-PI: J. Xu (Brown) No
overlap with the proposal being submitted. Role: PI
Soloman Grant J.I. Yeh 01/01/2000 Structural studies on proteins mediating key metabolic
pathways in pathogenic bacteria. P.I. J.I.Yeh
Rhode Island Foundation J.I. Yeh 06/01/1999 PI J.I.Yeh
Equipment Grants:
MF-29305 01/01/99 Markey Foundation Acquisition of Diffraction System for Structural Biology and
Biochemistry
NSF-0116441 D. Cane 09/01/01 NSF Major Research Instrumentation Acquisition of a High Resolution
Mass Spectrometer Role: co-PI (J. Yeh, J. Martin, C. Seto, A. Basu)
PHS 398/2590 (Rev. 11/07)
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