* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Genetics Laboratory (BIOL 311L)
Survey
Document related concepts
Gel electrophoresis wikipedia , lookup
Molecular evolution wikipedia , lookup
Agarose gel electrophoresis wikipedia , lookup
Non-coding DNA wikipedia , lookup
Molecular cloning wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Bisulfite sequencing wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Transformation (genetics) wikipedia , lookup
DNA supercoil wikipedia , lookup
Gel electrophoresis of nucleic acids wikipedia , lookup
Real-time polymerase chain reaction wikipedia , lookup
Restriction enzyme wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Transcript
Genetics Laboratory (BIOL 311L) Course description: Laboratory experience to illustrate and explain the principles covered in BIOL 311. Prerequisite or co-requisite: BIOL 311. One semester; one credit. DATE Week 1 Assigned reading/resource TOPIC/TASK Review of the syllabus Lab rules, p.1 http://www.phys.ksu.edu/gene/a1.html Yeast simple cross, pp. 2-7 (will come into lab twice briefly during the week) Introduction to S. cerevisiae, cross Media preparation (YED, MIN, YEKAC) Finish Yeast simple cross, p. 8 Finish observation of yeast life cycle, 2 x 2 segregation Introduce mapping Week 2 Brief intro into plasmids/restriction enzymes, p. 9 http://www.carolina.com/pdf/activities-articles/plasmidmapping-exercises-2008.pdf MOVIE: Cracking Your Genetic Code Restriction mapping exercise, pp. 10-12 Week 3 Digest plasmid DNA with four different enzymes, run gels Chromosome mapping (lecture support) Continue restriction mapping exercise, pp. 9-14 Do double digests of plasmid DNA, run gels E. coli/responsible handling of E. coli, pp. 15-16 Sterile technique, serial dilutions, pp. 17-19 Begin preparation for materials needed in Ames Test Lab report of restriction mapping exercise due Begin work with bacterial cultures Prepare media Ames Test: pp. 20-29 (come in next day to remove serial dilution plates from incubator; remove assay plates after 48 hours and choose one colony for further study) Serial dilution The assay with suspected mutagens Polymerase Chain Reaction, p. 30-31 Revertion--Phenotype to DNA, pp. 32–37 Counting CFUs DNA isolation PCR of mutant DNA Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 MIDTERM LAB EXAM/PRACTICAL FALL BREAK! Compare sequences of Ames Test revertants Week 10 http://www.silencinggenomes.org/ Return to culturing E. coli, pp. 40-41 C. elegans, p. 42 Week 11 http://www.wormclassroom.org/short-history-c-elegansresearch Observing wild-type and mutant C. elegans, pp. 43-48 Culturing C. elegans, pp. 49-52 Compose list of carcinogens and mutations created Seed bacteria Prepare media Observing/chunking/ picking C. elegans Week 12 RNA interference, p. 54-58 Inducing RNAi by feeding, pp. 59-68 http://www.pbs.org/wgbh/nova/sciencenow/3210/02.html Seeding, chunking, and picking worms Bioinformatics exercise Inducing RNAi by feeding, p. 67-68 Finish gathering of RNA silencing results DNA extraction, amplification of PTC gene locus Week 13 Analysis of the human PTC locus, pp. 69-70 http://www.dnalc.org/resources/animations/index.html DNA digestion with Fnu4HI, p. 70 Week 14 Fingerprint Ridge Counts, pp. 72-77 http://www.migeneticsconnection.org/genomics/ Mulitfactorial Traits/Fingerprint Ridge Count.htm Gel electrophoresis of amplified PTC DNA, p. 71 Week 15 Population genetics, p. 78-80 Film: Genetics of Bitter Taste Perception http://www.youtube.com/watch?v=PGoBr0usBzA Week 16 Restriction digest of PCR fragments Prepare own fingerprints, calculate TRC (10 pt. data sheets for turn-in) Run PCR/digested DNA on gel PTC tasting FINAL LAB EXAM/PRACTICAL