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Download RFLP - Science
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RFLP • “Restriction Fragment Length Polymorphism” • Basic idea: – If two DNA sequences have different numbers or locations of restriction sites, they will differ in their restriction enzyme digest pattern • Uses: – Paternity testing – Criminal cases – Locating disease genes Criminal Case http://www.scientific.org/case-in-point/articles/thompson/ Paternity testing Child 2 Child 1 Dad Mom Recombinant DNA • Engineered pieces of DNA inserted into the genomes of a target organisms • Can be used to mass produce useful proteins – “Humulin” – human insulin produced by bacteria (Eli Lilly company) – Human growth hormone • Also used to modify organisms • All originally depends on restriction enzymes! GloFish
