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Transcript
Proteome Atlas of Pigeonpea: Developmental proteins Sufia Farhat1*, Nisha Singh1, Sangeeta Singh1, Neha Jain1, Nagendra Kumar Singh1, Vandna Rai1 1 * Genomics and Proteomics Lab, National Research Centre on Plant Biotechnology, IARI, New Delhi, India Summary: Pigeonpea is the second most important food legume crop of India but its productivity has remained stagnated for the last five decades. There is need to improve its yield potential by changing the plant type and harvest index. In the present study we have developed complete proteome atlas of root and shoot of young seedlings of pigeonpea. As a first step we have identified the proteins of mature seeds and seedlings. A gel free LC-MS analysis were employed for identifying proteins of the tissues. The proteins were extracted from seeds, shoot and root after 10 days of germination. Total 78, 97 and 58 proteins were identified in seed, root and shoot, respectively. The maximum proteins were found for seed followed by root and shoot. Among them 45 proteins were common to all the 3 samples. The uncharacterized proteins were maximum for shoot tissues. Interestingly Dof family of proteins were significantly observed for all the samples. There are 38 Dof proteins are present in pigeonpea genome, among them 13 were reported for seed, root and shoot samples. DNA binding with One Finger (Dof) protein is a plant specific transcription factor having diverse role in plant growth and development. An attempt to characterize CcDof gene family using various bioinfomatic tools like protein motif analysis, phylogenetic analysis and secondary structure analysis has been made. The structural, functional and in-silico studies of Dof transcription factor attempted here exhibits great opportunity to analyse the pigeonpea genome in terms of number of Dof genes and shed light on evolution of legume crops. The multiple functions of Dof genes and their role and position in the metabolic pathways needs to be explored for crop improvement.