Download AB205Abstract_proteomics_conference

Pathogenies Related class 10 Proteins: Molecular insights and potential application in
abiotic stress tolerance
Shalu Jain1, Anjana Chaurasia1, Deepak Kumar1,3, Shashi Shekhar1, Mukesh Jain1, Prerna Chaudhary1,
Neelam P Negi1, Sanjeev Srivastava2, Nat N. V. Kav2, Neera Bhalla Sarin1*
1
Plant Developmental Biology and Transformation Lab, School of Life Sciences, Jawaharlal Nehru
University, New Delhi 110067, India
2
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton,
Alberta T6G 2P5, Canada
3
Department of Plant Sciences, Central University of Jammu, Jammu-180011
*e-mail: [email protected]
Abstract
Abiotic stresses such as salinity, drought, heat, cold and heavy metals are major constraints for
sustainable agricultural production and food security. Studying plant responses to these adverse
conditions can help in determining the strategies to combat them. A proteomic approach was
used to compare protein expression between Arachis hypogaea callus cell lines adapted to
salinity stressand control cell lines. A large number of proteins were identified as pathogenesis
related (PR) proteins belonging to class 10 and suggested that these differentially phosphorylated
proteins play a crucial role in plant defense system against salinity stress. Full length PR10 was
isolated (474 bp) from cell lines, and was named AhSIPR10 (GenBank accession number:
DQ813661)). Multiple sequence alignments pointed out phylogenetic affiliations of AhSIPR10
with several other PR10 family members and presence of conserved class 10 specific protein
motifs. Differential expression analysis of AhSIPR10 using qPCR indicated rapid upregulation of
AhSIPR10 in peanut callus cell lines across salinity, heavy metal, cold and mannitol-induced
drought stress environments. Likewise, AhSIPR10 mRNAexpression also responded to
defense/stress signaling molecules, including salicylic acid (SA), abscisic acid (ABA) and
methyl jasmonate. A functional validation of role of AhSIPR10 protein in alleviating abiotic
stress was carried through its over-expression in different transgenic systems. Overexpression of
the AhSIPR10 gene in transgenic tobacco and banana plants resulted in enhanced tolerance to salt
and drought stress. These results indicated that the AhSIPR10 protein played an important role in
the defense mechanism of plants against multiple abiotic stresses. Further characterization of
AhSIPR10 gene and its regulation under other abiotic and biotic stress environments will
enhance our understanding of the molecular cross-talk among various defense pathways.